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AB326646

Anti-Histone H2B (phospho T129) antibody [SR1184]

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Rabbit Recombinant Monoclonal H2B1 phospho T129 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae S288C samples.

View Alternative Names

H2B1, SPT12, YDR224C, YD9934.09C, HTB1, Histone H2B.1, Suppressor of Ty protein 12

1 Images
Western blot - Anti-Histone H2B (phospho T129) antibody [SR1184] (AB326646)
  • WB

Supplier Data

Western blot - Anti-Histone H2B (phospho T129) antibody [SR1184] (AB326646)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Saccharomyces cerevisiae cell lysate treated with Methyl methanesulfonate.
lysate/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 14 kDa
Observed band size : 14 kDa
Exposure time : 4 seconds

All lanes:

Western blot - Anti-Histone H2B (phospho T129) antibody [SR1184] (ab326646) at 1/1000 dilution

All lanes:

Saccharomyces cerevisiae cell lysate treated with Methyl methanesulfonate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

Exposure time: 4s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR1184

Isotype

IgG

Carrier free

No

Reacts with

Saccharomyces cerevisiae S288C

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae S288C": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information HTB1 phospho T129
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com