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Rabbit Recombinant Monoclonal H2B antibody. Carrier free. Suitable for ChIP, WB, PepArr and reacts with Saccharomyces cerevisiae, Recombinant fragment samples.

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Images

ChIP - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (AB250944), expandable thumbnail
  • Peptide Array - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (AB250944), expandable thumbnail
  • Western blot - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (AB250944), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPWBPepArr
Recombinant fragment
Not recommended
Not recommended
Tested
Saccharomyces cerevisiae
Tested
Tested
Expected

Tested
Tested

Species
Saccharomyces cerevisiae
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment
Dilution info
-
Notes

-

Tested
Tested

Species
Saccharomyces cerevisiae
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment
Dilution info
-
Notes

-

Expected
Expected

Species
Saccharomyces cerevisiae
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

H2B1, SPT12, YDR224C, YD9934.09C, HTB1, Histone H2B.1, Suppressor of Ty protein 12

Recommended products

Rabbit Recombinant Monoclonal H2B antibody. Carrier free. Suitable for ChIP, WB, PepArr and reacts with Saccharomyces cerevisiae, Recombinant fragment samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR18094
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250944 is the carrier-free version of Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade ab188291.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • ChIP - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944), expandable thumbnail

    ChIP - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)

    This data was developed using Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade ab188291, the same antibody clone in a different buffer formulation.Chromatin was prepared from Saccharomyces cerevisiae cells according to the Abcam X-ChIP protocol. Saccharomyces cerevisiae cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade ab188291 (red), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

  • Peptide Array - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944), expandable thumbnail

    Peptide Array - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)

    This data was developed using Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade ab188291, the same antibody clone in a different buffer formulation.Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade ab188291 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.

  • Western blot - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944), expandable thumbnail

    Western blot - Anti-Histone H2B (yeast) antibody [EPR18094] - BSA and Azide free (ab250944)

    This data was developed using Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade ab188291, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade ab188291) at 1/2000 dilution

    All lanes: Saccharomyces cerevisiae whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Observed band size: 14 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com