AB203952

Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free

RabMAb
Advanced Validation
Recombinant
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(14 Publications)

Rabbit Recombinant Monoclonal H3 acetyl K14 antibody. Carrier free. Suitable for PepArr, IHC-P, ChIP, WB, ICC/IF and reacts with Synthetic peptide, Mouse, Rat, Human samples. Cited in 14 publications.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K14ac

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

ab52946 at 1/100 dilution staining Histone H3 (acetyl K14) in human uterus adenocarcinoma tissue by Immunohistochemistry, Paraffin embedded tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52946).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

Immunocytochemistry/Immunofluorescence analysis of untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cells and TSA (Trichostatin A) (500ng/ml, 4h) and treated HeLa cells labeling Histone H3 (acetyl K14) with purified ab52946 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100, counterstained with ab150120 AlexaFluor^®594 Goat anti-Mouse secondary 1 : 1000 (2ug/ml). An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

Negative Control 1 : Rabbit primary antibody and anti-mouse secondary antibody(ab150120)

Negative Control 2 : Mouse primary antibody(ab7291) and anti-rabbit secondary antibody(ab150077)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52946).

ChIP - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

ChIP

Unknown

ChIP - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

This data was developed using the same antibody clone in a different buffer formulation (ab52946).

Chromatin was prepared from HeLa cells (treated with 50 ng/ml nocodazole for 14 hours) according to the Abcam X-ChIP protocol. Cells were fixed with EGS (1.5 mM) for 30 minutes then formaldehyde (1%) for 10 minutes. The ChIP was performed with 25μg of chromatin, 5μg of ab52946 (red), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (TaqMan approach).

Lab

Western blot - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

This data was developed using ab52946, the same antibody clone in a different buffer formulation. Different batches of ab52946 were tested on C6 (Rat glial tumor glial cell) treated with Trichostatin A lysate at 1.2 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 15 kDa.

All lanes:

Western blot - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-acetyl-k14-antibody-ep964y-chip-grade-ab52946'>ab52946</a>)

Predicted band size: 15 kDa

false

Peptide Array - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

PepArr

Lab

Peptide Array - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

This data was developed using the same antibody clone in a different buffer formulation (ab52946). ab52946 at 0.01 μg/mL was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). A Goat Anti-Rabbit IgG, (H+L), Fluo 647nm conjugated at 1/50000 dilution was used as the secondary antibody. Washing buffer and time was protein Array Washing Buffer, 2*10mins and 2*5mins and blocking buffer was 5% BSA in TBST. Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.

Scanner details : Innoscan 710

Unconjugated

Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade
660 APC

APC Anti-Histone H3 (acetyl K14) antibody [EP964Y] – ChIP Grade
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Histone H3 (acetyl K14) antibody [EP964Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Histone H3 (acetyl K14) antibody [EP964Y] – ChIP Grade
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Histone H3 (acetyl K14) antibody [EP964Y] – ChIP Grade
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Histone H3 (acetyl K14) antibody [EP964Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade
Biotin

Biotin Anti-Histone H3 (acetyl K14) antibody [EP964Y]
578 PE

PE Anti-Histone H3 (acetyl K14) antibody [EP964Y] – ChIP Grade

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP964Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ChIP, ICC/IF, WB, IHC-P, PepArr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

There was no cross-reactivity observed with recombinant H3 or the following modifications Acetyl-K9/pS10, -K18, -K23, and -K27 in dot plot.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "5 µg for 25 µg chromatin", "ChIP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Saccharomyces cerevisiae": { "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Schizosaccharomyces pombe": { "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab203952 is the carrier-free version of ab52946.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 acetyl K14

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1209 PubMed40804340

2025

rTMS ameliorates cerebral ischemia-reperfusion injury by inhibiting Golgi apparatus stress through epigenetic modulation of Gli2.

Applications

Unspecified application

Species

Unspecified reactive species

Chunjiao Zhu,Yongmei Fan,Wenna Peng

Biology direct 19:74 PubMed39183358

2024

SUMO3 inhibition by butyric acid suppresses cell viability and glycolysis and promotes gemcitabine antitumor activity in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Zhu,Gang Chen,Changjing Huang,Huifeng Gao,Yilin Wang,Yehua Shen

Molecular medicine reports 23: PubMed33880587

2021

H3K14 hyperacetylation‑mediated c‑Myc binding to the miR‑30a‑5p gene promoter under hypoxia postconditioning protects senescent cardiomyocytes from hypoxia/reoxygenation injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lingbo Xu,Huiping Zhang,Yanhua Wang,Wei Guo,Lingyu Gu,Anning Yang,Shengchao Ma,Yong Yang,Kai Wu,Yideng Jiang

The Journal of biological chemistry 289:15798-809 PubMed24759103

2014

New role for Kruppel-like factor 14 as a transcriptional activator involved in the generation of signaling lipids.

Applications

ChIP

Species

Human

Thiago M de Assuncao,Gwen Lomberk,Sheng Cao,Usman Yaqoob,Angela Mathison,Douglas A Simonetto,Robert C Huebert,Raul A Urrutia,Vijay H Shah

Nucleic acids research 42:1656-70 PubMed24234443

2013

RNA-dependent dynamic histone acetylation regulates MCL1 alternative splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Dilshad H Khan,Carolina Gonzalez,Charlton Cooper,Jian-Min Sun,Hou Yu Chen,Shannon Healy,Wayne Xu,Karen T Smith,Jerry L Workman,Etienne Leygue,James R Davie

Molecular and cellular biology 33:1198-209 PubMed23319049

2013

FOSL1 controls the assembly of endothelial cells into capillary tubes by direct repression of αv and β3 integrin transcription.

Applications

Unspecified application

Species

Human

Sandrine Evellin,Federico Galvagni,Alessio Zippo,Francesco Neri,Maurizio Orlandini,Danny Incarnato,Daniela Dettori,Stefanie Neubauer,Horst Kessler,Erwin F Wagner,Salvatore Oliviero

The Journal of biological chemistry 287:41469-80 PubMed22982396

2012

Oncogene Ras/phosphatidylinositol 3-kinase signaling targets histone H3 acetylation at lysine 56.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Da-Liang Wang,Su Chen,Lei Zhao,Fang-Lin Sun

PloS one 7:e33249 PubMed22438904

2012

Age-related memory impairment is associated with disrupted multivariate epigenetic coordination in the hippocampus.

Applications

Species

Rat

James F Castellano,Bonnie R Fletcher,Bennett Kelley-Bell,David H Kim,Michela Gallagher,Peter R Rapp

The EMBO journal 30:4287-98 PubMed21847097

2011

CBP is required for environmental enrichment-induced neurogenesis and cognitive enhancement.

Applications

Unspecified application

Species

Unspecified reactive species

Jose P Lopez-Atalaya,Alessandro Ciccarelli,Jose Viosca,Luis M Valor,Maria Jimenez-Minchan,Santiago Canals,Maurizio Giustetto,Angel Barco

Biochemical and biophysical research communication 406:444-8 PubMed21333630

2011

Sde2: a novel nuclear protein essential for telomeric silencing and genomic stability in Schizosaccharomyces pombe.

Applications

ChIP

Species

Schizosaccharomyces pombe

Rie Sugioka-Sugiyama,Tomoyasu Sugiyama

View all publications

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peptideArrayWebsite

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

ChIP - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

Western blot - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

Peptide Array - Anti-Histone H3 (acetyl K14) antibody [EP964Y] - BSA and Azide free (AB203952)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (14)

Product promise