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Rabbit Recombinant Monoclonal H3 acetyl K14 antibody. Suitable for Protein Array, IHC-P, ChIP, WB, ICC/IF and reacts with , Mouse, Rat, Human samples. Cited in 104 publications.
Alternative names=Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3E, H3FI, H3FD, H3C6, HIST1H3D, H3FB, H3C4, HIST1H3C, H3FC, H3C3, HIST1H3B, H3FL, H3C2, HIST1H3A, H3FA, H3C1, H3C7, HIST1H3F, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3K14ac
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Protein Array | IHC-P | ChIP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Expected | Tested | Expected | Not recommended | Expected | Expected |
| Rat | Expected | Tested | Expected | Not recommended | Tested | Expected |
Tested | ||||||
| Caenorhabditis elegans | Predicted | Predicted | Predicted | Not recommended | Predicted | Predicted |
| Drosophila melanogaster | Predicted | Predicted | Predicted | Not recommended | Predicted | Predicted |
| Saccharomyces cerevisiae | Predicted | Predicted | Predicted | Not recommended | Predicted | Predicted |
| Schizosaccharomyces pombe | Predicted | Predicted | Predicted | Not recommended | Predicted | Predicted |
Images
Protein Array
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg chromatin for 25 µg chromatin | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/2000 | Notes - |
Species Human | Dilution info 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe | Dilution info - | Notes - |
Recommended products
Rabbit Recombinant Monoclonal H3 acetyl K14 antibody. Suitable for Protein Array, IHC-P, ChIP, WB, ICC/IF and reacts with , Mouse, Rat, Human samples. Cited in 104 publications.
Alternative names=Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3E, H3FI, H3FD, H3C6, HIST1H3D, H3FB, H3C4, HIST1H3C, H3FC, H3C3, HIST1H3B, H3FL, H3C2, HIST1H3A, H3FA, H3C1, H3C7, HIST1H3F, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3K14ac
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EP964Y
- Purification technique
Affinity purification Protein A
- Specificity
There was no cross-reactivity observed with recombinant H3 or the following modifications Acetyl-K9/pS10, -K18, -K23, and -K27 in dot plot.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Stable for 12 months at -20°C
Notes
Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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