AB1191

Anti-Histone H3 (acetyl K18) antibody - ChIP Grade

Name: Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191) | Abcam
Brand: Abcam Limited
SKU: AB1191
Price: 435 USD
Availability: InStock
Rating: 4 (8 reviews)

BOND RX™ Validated
What is this?

(8 Reviews)

(225 Publications)

Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ChIP, PepArr. Suitable for Arabidopsis thaliana, Human, Mouse.

- Over 190 publications
- Trusted since 2003

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K18ac

6 Images

Ap796247****

Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

All lanes:

Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191) at 1 µg/mL

Lanes 1, 3, 6, 7 and 9:

HeLa Histone Preparation Nuclear Lysate at 2.5 µg

Lane 2:

HeLa Histone Preparation Nuclear Lysate at 2.5 µg with Histone H3 peptide - unmodified R17

Lane 4:

HeLa Histone Preparation Nuclear Lysate at 2.5 µg with Histone H3 peptide - acetyl K14

Lane 5:

HeLa Histone Preparation Nuclear Lysate at 2.5 µg with Human Histone H3 (acetyl K18) peptide (<a href='/en-us/products/unavailable/human-histone-h3-acetyl-k18-peptide-ab24003'>ab24003</a>)

Lane 8:

HeLa Histone Preparation Nuclear Lysate at 2.5 µg with Histone H3 peptide - acetyl K36

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 1min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

IHC image of Histone H3 (acetyl K18) staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1191, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

ab1191 staining Histone H3 (acetyl K18) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab1191 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

ChIP - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

ChIP

Unknown

ChIP - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 2 μg of ab1191 (blue), and 20 μl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

Project900****

Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

All lanes:

Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191) at 1 µg/mL

Lane 1:

Histone prep at 0.5 µg/mL

Lane 2:

Histone prep at 0.5 µg/mL with Human Histone H3 (acetyl K18) peptide (<a href='/en-us/products/unavailable/human-histone-h3-acetyl-k18-peptide-ab24003'>ab24003</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Peptide Array - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

PepArr

Lab

Peptide Array - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

All batches of ab1191 are tested in Peptide Array against peptides to different Histone H3 and H4 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - acetyl K18 peptide (ab24003), indicating that this antibody specifically recognises the Histone H3 - acetyl K18 modification.

ab17163 - Histone H3 - unmodified

ab16635 - Histone H3 - acetyl K9

ab15591 - Histone H3 - acetyl K14

ab24003 - Histone H3 - acetyl K18

ab48359 - Histone H3 - acetyl K23

ab24404 - Histone H3 - acetyl K27

ab41409 - Histone H3 - acetyl K36

ab15662 - Histone H4 - acetyl K12

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Arabidopsis thaliana

Applications

WB, IHC-P, PepArr, ICC/IF, ChIP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Specificity for acetyl K18 has been validated by blocking peptide/antibody incubation followed by immunostaining western blots.

From September 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2 µg for 10^6 Cells", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "0.02-0.2 µg/mL", "PepArr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "2 µg for 10^6 Cells", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "0.02-0.2 µg/mL", "PepArr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Arabidopsis thaliana": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "2 µg for 10^6 Cells", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "0.02-0.2 µg/mL", "PepArr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Cow": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Fungi": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Saccharomyces cerevisiae": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP, Peptide Array in Arabidopsis thaliana, Human, Mouse samples.

Trusted by the scientific community
Anti-Histone H3 (acetyl K18) - ChIP Grade (ab1191) was first used in a scientific publication in 2003 and has been cited over 190 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H3 (acetyl K18) - ChIP Grade (ab1191) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 acetyl K18

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Publications (225)

Recent publications for all applications. Explore the full list and refine your search

Epigenetics & chromatin 18:62 PubMed41013562

2025

Antibiotics induce overexpression of alpha satellite DNA accompanied with epigenetic changes at alpha satellite arrays as well as genome-wide.

Applications

Unspecified application

Species

Unspecified reactive species

Sven Ljubić,Maja Matulić,Damir Đermić,Maria Chiara Feliciello,Alfredo Procino,Đurđica Ugarković,Isidoro Feliciello

mBio 16:e0187125 PubMed40823836

2025

Validation of human sensory neurons derived from inducible pluripotent stem cells as a model for latent infection and reactivation by herpes simplex virus 1.

Applications

Unspecified application

Species

Unspecified reactive species

Hyung Suk Oh,Shu-Fan Chou,Priya Raja,Jaehoon Shim,Biswajit Das,Jean M Pesola,Nicolás Romero,Jennifer S Lee,Alex Ng,Elizabeth D Buttermore,George Church,Clifford J Woolf,Donald M Coen,David M Knipe

RSC medicinal chemistry 16:2049-2060 PubMed40093518

2025

Development of p300-targeting degraders with enhanced selectivity and onset of degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Graham P Marsh,Mark S Cooper,Sean Goggins,Stephen J Reynolds,Dean F Wheeler,Joel O Cresser-Brown,Robert E Arnold,Emily G Babcock,Gareth Hughes,Darko Bosnakovski,Michael Kyba,Samuel Ojeda,Drew A Harrison,Christopher J Ott,Hannah J Maple

Life medicine 3:lnae033 PubMed39872440

2025

Deciphering the dynamic single-cell transcriptional landscape in the ocular surface ectoderm differentiation system.

Applications

Unspecified application

Species

Unspecified reactive species

Canwei Zhang,Zesong Lin,Yankun Yu,Siqi Wu,Huaxing Huang,Ying Huang,Jiafeng Liu,Kunlun Mo,Jieying Tan,Zhuo Han,Mingsen Li,Wei Zhao,Hong Ouyang,Xiangjun Chen,Li Wang

Nature communications 16:887 PubMed39856033

2025

Chemical catalyst manipulating cancer epigenome and transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Yamanashi,Shinpei Takamaru,Atsushi Okabe,Satoshi Kaito,Yuto Azumaya,Yugo R Kamimura,Kenzo Yamatsugu,Tomoya Kujirai,Hitoshi Kurumizaka,Atsushi Iwama,Atsushi Kaneda,Shigehiro A Kawashima,Motomu Kanai

The Journal of biological chemistry 301:108173 PubMed39798880

2025

Maternal obesity alters histone modifications mediated by the interaction between EZH2 and AMPK, impairing neural differentiation in the developing embryonic brain cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Thilina T Alawathugoda,Muhammad Abid Sheikh,Anil Kumar Challagandla,S Thameem Dheen,Bright Starling Emerald,Suraiya Anjum Ansari

The New phytologist 245:1589-1607 PubMed39639540

2024

Epigenetic regulation of lignin biosynthesis in wood formation.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Ma,Liwei Su,Wen Zhang,Yi Sun,Danning Li,Shuang Li,Ying-Chung Jimmy Lin,Chenguang Zhou,Wei Li

Molecular cancer 23:222 PubMed39367409

2024

Pharmacological targeting of P300/CBP reveals EWS::FLI1-mediated senescence evasion in Ewing sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Erdong Wei,Ana Mitanoska,Quinn O'Brien,Kendall Porter,MacKenzie Molina,Haseeb Ahsan,Usuk Jung,Lauren Mills,Michael Kyba,Darko Bosnakovski

The Journal of cell biology 223: PubMed39133301

2024

Histone H4 acetylation differentially modulates proliferation in adult oligodendrocyte progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

David K Dansu,Ipek Selcen,Sami Sauma,Emily Prentice,Dennis Huang,Meng Li,Sarah Moyon,Patrizia Casaccia

iScience 27:110014 PubMed38947512

2024

YZL-51N functions as a selective inhibitor of SIRT7 by NAD competition to impede DNA damage repair.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Shu Kang,Yong-Ming Yan,Yuan Tian,Jun Zhang,Minghui Zhang,Yuxin Shu,Jinbo Huang,Jing He,Cheng-Tian Tao,Qian Zhu,Jinke Gu,Xiaopeng Lu,Yong-Xian Cheng,Wei-Guo Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (acetyl K18) antibody - ChIP Grade

Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

ChIP - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

Peptide Array - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (AB1191)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (225)

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