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Rabbit anti-Histone H3 antibody ab4729 is a rabbit polyclonal antibody that is used in Histone H3 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

Tried and trusted by researchers since 2004
Anti-Histone H3 antibody ab4729 is cited in over 2270 publications
Same trusted quality, New lower price!


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PChIPWBPepArrICC/IF
Human
Tested
Tested
Tested
Predicted
Tested
Mouse
Expected
Expected
Tested
Predicted
Expected
Rat
Expected
Expected
Tested
Predicted
Expected
Arabidopsis thaliana
Predicted
Predicted
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Predicted
Predicted
Cow
Expected
Expected
Tested
Predicted
Expected
Cyanidioschyzon merolae
Predicted
Predicted
Predicted
Predicted
Predicted
Drosophila melanogaster
Predicted
Predicted
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Predicted
Predicted
Plasmodium falciparum
Predicted
Predicted
Predicted
Predicted
Predicted
Rice
Predicted
Predicted
Predicted
Predicted
Predicted
Synthetic peptide - Human
Not recommended
Not recommended
Not recommended
Expected
Not recommended
Xenopus laevis
Predicted
Predicted
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

-

Notes

-

Species

Cow

Dilution info

-

Notes

-

Species

Mouse

Dilution info

1 µg/mL

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Chicken, Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Monkey, Zebrafish, Rice, Cyanidioschyzon merolae, Plasmodium falciparum

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

2 µg chromatin for 25 µg chromatin

Notes

We recommend GAPDH positive control ChIP primer pair ab267832 as a positive control.

Expected
Expected

Species

Rat

Dilution info

-

Notes

-

Species

Cow

Dilution info

-

Notes

-

Species

Mouse

Dilution info

2 µg for 25 µg chromatin

Notes

-

Predicted
Predicted

Species

Chicken, Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Monkey, Zebrafish, Rice, Cyanidioschyzon merolae, Plasmodium falciparum

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1 µg/mL

Notes

We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody.

Species

Human

Dilution info

1 µg/mL

Notes

We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody.

Species

Cow

Dilution info

1 µg/mL

Notes

We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody.

Species

Mouse

Dilution info

1 µg/mL

Notes

We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody.

Predicted
Predicted

Species

Chicken, Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Monkey, Zebrafish, Rice, Cyanidioschyzon merolae, Plasmodium falciparum

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Synthetic peptide - Human

Dilution info

0.02000-0.20000 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow, Human, Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Monkey, Zebrafish, Rice, Cyanidioschyzon merolae, Plasmodium falciparum

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.5 µg/mL

Notes

Can be used with paraformaldehyde- or methanol- fixed cells.

Expected
Expected

Species

Rat

Dilution info

-

Notes

-

Species

Cow

Dilution info

-

Notes

-

Species

Mouse

Dilution info

0.5 µg/mL

Notes

Can be used with paraformaldehyde- or methanol- fixed cells.

Predicted
Predicted

Species

Chicken, Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Monkey, Zebrafish, Rice, Cyanidioschyzon merolae, Plasmodium falciparum

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

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Rabbit anti-Histone H3 antibody ab4729 is a rabbit polyclonal antibody that is used in Histone H3 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

Tried and trusted by researchers since 2004
Anti-Histone H3 antibody ab4729 is cited in over 2270 publications
Same trusted quality, New lower price!

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

Histone H3 participates in chromatin remodeling and gene transcription regulation. As part of the nucleosome complex it influences DNA accessibility and subsequently gene expression. Through post-translational modifications including methylation and phosphorylation H3 modulates chromatin structure. Common modifications include mono- di- and tri-methylation at various lysine residues like K56 which affect transcriptional activation or repression. These modifications form a part of the histone code a framework for regulating chromatin dynamics and genetic information.

Activity summary

Histone H3 is a core component of the nucleosome playing an important role in DNA packaging within eukaryotic cells. Often referred to as H3 it has a molecular weight of around 15 kDa. Histone H3 is expressed in the nucleus of nearly all eukaryotic cells where it interacts with DNA and other histone proteins to form chromatin structure. Scientists frequently study histone H3 using techniques like Western blotting and immunohistochemistry utilizing antibodies such as anti-histone or phospho-histone H3 to distinguish post-translational modifications.

Pathways

Histone H3 modifications are integral to several signaling pathways that modulate gene expression and cellular responses. The histone code interpreted by other proteins such as transcription factors and chromatin remodelers contributes significantly to the regulation of the cell cycle and signal transduction pathways like the MAPK/ERK pathway. H3 interacts closely with regulatory proteins including other histones and transcription machinery to facilitate these complex processes.

Associated diseases and disorders

Histone H3 modifications are associated with various health conditions including cancer and neurological disorders. Aberrations in histone H3 methylation are linked to oncogenic transformations with disruptions often found in cancer types like glioblastoma and breast cancer. The alterations in H3 also connect to Alzheimer's disease through interactions with other histone modifications and their impact on gene expression patterns critical for neural function. Understanding these modifications helps provide insights into disease mechanisms and potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729), expandable thumbnail

    ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 2 μg of ab4729 (blue), and 20 μl of Protein A/G sepharose beads.

    No antibody was added to the beads control (yellow).

    The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729), expandable thumbnail

    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were incubated at 37°C for 6 hours with vehicle control (0 μM) and different concentrations of sodium butyrate (ab120948). Increased expression of histone H3 (acetyl K27)(ab4729) in HeLa cells correlates with an increase in sodium butyrate concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 2.5 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab4927 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10,000 dilution and visualised using ECL development solution.

    All lanes: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729)

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa

    Observed band size: 17 kDa

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    IHC image of ab4729 staining Histone H3 (acetyl K27) in human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer pH 6 for 20 minutes. The section was then incubated with ab4729, 5 μg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729), expandable thumbnail

    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    All lanes: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729) at 1/2500 dilution

    Lane 1: Untreated Mouse MEF cell lysate at 9 µg

    Lane 2: 0.4 µM Trichostatin A treatment for 18 hr Mouse MEF cell lysate at 9 µg

    Secondary

    All lanes: Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB6802) at 1/20000 dilution

    Predicted band size: 15 kDa

  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729), expandable thumbnail

    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    All lanes: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729) at 1 µg/mL

    All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparation, nuclear Lysate - Butyrate treated at 2.5 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa

    Observed band size: 17 kDa

    Exposure time: 10s

  • Peptide Array - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729), expandable thumbnail

    Peptide Array - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    All batches of ab4729 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - acetyl K27 peptide (ab24404), indicating that this antibody specifically recognises the Histone H3 - acetyl K27 modification.

    ab24404 - Histone H3 - acetyl K27

    ab15591 - Histone H3 - acetyl K14

    ab24003 - Histone H3 - acetyl K18

    ab17163 - Histone H3 unmodified

    ab48359 - Histone H3 - acetyl K23

    ab41409 - Histone H3 - acetyl K36

    ab15662 - Histone H4 - acetyl K12

    ab16635 - Histone H3 acetyl K9

  • ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729), expandable thumbnail
    This image is courtesy of an anonymous customer review

    ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    ChIP was performed with human thyroid cancer cell lysate and 1 µg/µg of ab4729. Lysates were incubated with the primary antibody for 16 hours at 4°C. positive control promoter: GAPDH promoter; positive control enhancer: enhancer region of a published target (10.1093/nar/gkx802); negative control: published negative region (10.1093/nar/gkx802).

  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729), expandable thumbnail

    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    ab4729 specifically recognises acetyl K27 histone H3 in catlf thymus histone lysate, which is specifically blocked using the immunizing peptide ab24404.

    Lanes 1 and 3: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729) at 0.2 µg/mL

    Lanes 2 and 4: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729) at 0.1 µg/mL

    All lanes: Calf thymus histone lysate at 1 µg

    Secondary

    All lanes: Goat anti-rabbit (HRP) at 1/2000 dilution

    Predicted band size: 15 kDa

    Observed band size: 17 kDa

  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729), expandable thumbnail

    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    Blocking buffer : 2% BSA block
    Gel type : MES

    All lanes: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729) at 1 µg/mL

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) cell lysate - Sodium butyrate-treated at 10 µg

    Lane 2: HeLa (human cervix adenocarcinoma epithelial cell) nuclear lysate (triton enriched) at 10 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast cell line) nuclear lysate (triton enriched) at 10 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) nuclear lysate (triton enriched) at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 15 kDa

    Observed band size: 17 kDa

    Exposure time: 30s

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    ab4729 staining Histone H3 (acetyl K27) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4729 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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