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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit anti-Histone H3 antibody ab4729 is a rabbit polyclonal antibody that is used in Histone H3 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.
Tried and trusted by researchers since 2004
Anti-Histone H3 antibody ab4729 is cited in over 2270 publications
Same trusted quality, New lower price!
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
IHC-P | ChIP | WB | PepArr | ICC/IF | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Predicted | Tested |
Mouse | Expected | Expected | Tested | Predicted | Expected |
Rat | Expected | Expected | Tested | Predicted | Expected |
Arabidopsis thaliana | Predicted | Predicted | Predicted | Predicted | Predicted |
Chicken | Predicted | Predicted | Predicted | Predicted | Predicted |
Cow | Expected | Expected | Tested | Predicted | Expected |
Cyanidioschyzon merolae | Predicted | Predicted | Predicted | Predicted | Predicted |
Drosophila melanogaster | Predicted | Predicted | Predicted | Predicted | Predicted |
Monkey | Predicted | Predicted | Predicted | Predicted | Predicted |
Plasmodium falciparum | Predicted | Predicted | Predicted | Predicted | Predicted |
Rice | Predicted | Predicted | Predicted | Predicted | Predicted |
Synthetic peptide - Human | Not recommended | Not recommended | Not recommended | Expected | Not recommended |
Xenopus laevis | Predicted | Predicted | Predicted | Predicted | Predicted |
Zebrafish | Predicted | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species Cow | Dilution info - | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Monkey, Zebrafish, Rice, Cyanidioschyzon merolae, Plasmodium falciparum | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2 µg chromatin for 25 µg chromatin | Notes We recommend GAPDH positive control ChIP primer pair ab267832 as a positive control. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species Cow | Dilution info - | Notes - |
Species Mouse | Dilution info 2 µg for 25 µg chromatin | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Monkey, Zebrafish, Rice, Cyanidioschyzon merolae, Plasmodium falciparum | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody. |
Species Human | Dilution info 1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody. |
Species Cow | Dilution info 1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody. |
Species Mouse | Dilution info 1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Monkey, Zebrafish, Rice, Cyanidioschyzon merolae, Plasmodium falciparum | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide - Human | Dilution info 0.02000-0.20000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Cow, Human, Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Monkey, Zebrafish, Rice, Cyanidioschyzon merolae, Plasmodium falciparum | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.5 µg/mL | Notes Can be used with paraformaldehyde- or methanol- fixed cells. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species Cow | Dilution info - | Notes - |
Species Mouse | Dilution info 0.5 µg/mL | Notes Can be used with paraformaldehyde- or methanol- fixed cells. |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Monkey, Zebrafish, Rice, Cyanidioschyzon merolae, Plasmodium falciparum | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C10, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3FC, HIST1H3E, H3FD, H3C6, HIST1H3D, H3FB, H3C4, HIST1H3C, HIST1H3A, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FA, H3C3, HIST1H3B, H3FL, H3C2, H3K27ac
Rabbit anti-Histone H3 antibody ab4729 is a rabbit polyclonal antibody that is used in Histone H3 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.
Tried and trusted by researchers since 2004
Anti-Histone H3 antibody ab4729 is cited in over 2270 publications
Same trusted quality, New lower price!
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C10, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3FC, HIST1H3E, H3FD, H3C6, HIST1H3D, H3FB, H3C4, HIST1H3C, HIST1H3A, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FA, H3C3, HIST1H3B, H3FL, H3C2, H3K27ac
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Histone H3 participates in chromatin remodeling and gene transcription regulation. As part of the nucleosome complex it influences DNA accessibility and subsequently gene expression. Through post-translational modifications including methylation and phosphorylation H3 modulates chromatin structure. Common modifications include mono- di- and tri-methylation at various lysine residues like K56 which affect transcriptional activation or repression. These modifications form a part of the histone code a framework for regulating chromatin dynamics and genetic information.
Histone H3 is a core component of the nucleosome playing an important role in DNA packaging within eukaryotic cells. Often referred to as H3 it has a molecular weight of around 15 kDa. Histone H3 is expressed in the nucleus of nearly all eukaryotic cells where it interacts with DNA and other histone proteins to form chromatin structure. Scientists frequently study histone H3 using techniques like Western blotting and immunohistochemistry utilizing antibodies such as anti-histone or phospho-histone H3 to distinguish post-translational modifications.
Histone H3 modifications are integral to several signaling pathways that modulate gene expression and cellular responses. The histone code interpreted by other proteins such as transcription factors and chromatin remodelers contributes significantly to the regulation of the cell cycle and signal transduction pathways like the MAPK/ERK pathway. H3 interacts closely with regulatory proteins including other histones and transcription machinery to facilitate these complex processes.
Histone H3 modifications are associated with various health conditions including cancer and neurological disorders. Aberrations in histone H3 methylation are linked to oncogenic transformations with disruptions often found in cancer types like glioblastoma and breast cancer. The alterations in H3 also connect to Alzheimer's disease through interactions with other histone modifications and their impact on gene expression patterns critical for neural function. Understanding these modifications helps provide insights into disease mechanisms and potential therapeutic targets.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 2 μg of ab4729 (blue), and 20 μl of Protein A/G sepharose beads.
No antibody was added to the beads control (yellow).
The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were incubated at 37°C for 6 hours with vehicle control (0 μM) and different concentrations of sodium butyrate (ab120948). Increased expression of histone H3 (acetyl K27)(ab4729) in HeLa cells correlates with an increase in sodium butyrate concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 2.5 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab4927 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10,000 dilution and visualised using ECL development solution.
All lanes: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 10s
IHC image of ab4729 staining Histone H3 (acetyl K27) in human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer pH 6 for 20 minutes. The section was then incubated with ab4729, 5 μg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729) at 1/2500 dilution
Lane 1: Untreated Mouse MEF cell lysate at 9 µg
Lane 2: 0.4 µM Trichostatin A treatment for 18 hr Mouse MEF cell lysate at 9 µg
All lanes: Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB6802) at 1/20000 dilution
Predicted band size: 15 kDa
All lanes: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729) at 1 µg/mL
All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparation, nuclear Lysate - Butyrate treated at 2.5 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 10s
All batches of ab4729 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - acetyl K27 peptide (ab24404), indicating that this antibody specifically recognises the Histone H3 - acetyl K27 modification.
ab24404 - Histone H3 - acetyl K27
ab15591 - Histone H3 - acetyl K14
ab24003 - Histone H3 - acetyl K18
ab17163 - Histone H3 unmodified
ab48359 - Histone H3 - acetyl K23
ab41409 - Histone H3 - acetyl K36
ab15662 - Histone H4 - acetyl K12
ab16635 - Histone H3 acetyl K9
ChIP was performed with human thyroid cancer cell lysate and 1 µg/µg of ab4729. Lysates were incubated with the primary antibody for 16 hours at 4°C. positive control promoter: GAPDH promoter; positive control enhancer: enhancer region of a published target (10.1093/nar/gkx802); negative control: published negative region (10.1093/nar/gkx802).
ab4729 specifically recognises acetyl K27 histone H3 in catlf thymus histone lysate, which is specifically blocked using the immunizing peptide ab24404.
Lanes 1 and 3: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729) at 0.2 µg/mL
Lanes 2 and 4: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729) at 0.1 µg/mL
All lanes: Calf thymus histone lysate at 1 µg
All lanes: Goat anti-rabbit (HRP) at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa
Blocking buffer : 2% BSA block
Gel type : MES
All lanes: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729) at 1 µg/mL
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) cell lysate - Sodium butyrate-treated at 10 µg
Lane 2: HeLa (human cervix adenocarcinoma epithelial cell) nuclear lysate (triton enriched) at 10 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast cell line) nuclear lysate (triton enriched) at 10 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) nuclear lysate (triton enriched) at 10 µg
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 30s
ab4729 staining Histone H3 (acetyl K27) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4729 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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