Anti-Histone H3 (acetyl K36) antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K36) antibody (AB241055)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) stained for Histone H3 (acetyl K36) using ab241055 at 1/37.5 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).

• ChIP

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ChIP - Anti-Histone H3 (acetyl K36) antibody (AB241055)

Hela (human epithelial cell line from cervix adenocarcinoma; 4 x 10⁶, treated with 30mM sodium butyrate for 4hr) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 μg ab241055 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

• WB

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Western blot - Anti-Histone H3 (acetyl K36) antibody (AB241055)

All lanes:

Western blot - Anti-Histone H3 (acetyl K36) antibody (ab241055) at 1/500 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr

Lane 4:

Jurkat whole cell lysate, untreated (-)

Lane 5:

HEK-293 whole cell lysate, untreated (-)

Lane 6:

HepG2 whole cell lysate, untreated (-)

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/40000 dilution

Predicted band size: 15 kDa

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