Anti-Histone H3 (acetyl K36) antibody [EPR16992] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K36) antibody [EPR16992] - BSA and Azide free (AB249945)

This data was developed using ab177179, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Histone H3 (acetyl K36) with ab177179 at 1/100 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on Human skin tissue is observed. Counter stained with Hematoxylin.
Negative control : Using PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K36) antibody [EPR16992] - BSA and Azide free (AB249945)

This data was developed using ab177179, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling Histone H3 (acetyl K36) with ab177179 at 1/100 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green). Nuclear staining on HeLa cell line is observed. The expression increased after treatment with TSA (50 μg/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab177179 at 1/100 dilution followed by ab150120 (goat anti-mouse AlexaFluor®594 secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (goat anti-rabbit Alexa Fluor®488 (IgG H&L) at 1/400 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K36) antibody [EPR16992] - BSA and Azide free (AB249945)

This data was developed using ab177179, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H3 (acetyl K36) with ab177179 at 1/100 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of rat colon tissue is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K36) antibody [EPR16992] - BSA and Azide free (AB249945)

This data was developed using ab177179, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H3 (acetyl K36) with ab177179 at 1/100 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of mouse colon tissue is observed. Counter stained with Hematoxylin.
Negative control : Using PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Histone H3 (acetyl K36) antibody [EPR16992] - BSA and Azide free (AB249945)

This data was developed using ab177179, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Histone H3 (acetyl K36) antibody [EPR16992] (<a href='/en-us/products/primary-antibodies/histone-h3-acetyl-k36-antibody-epr16992-ab177179'>ab177179</a>) at 1/1000 dilution

Lane 1:

HeLa treated with Sodium butyrate extract lysates at 10 µg

Lane 2:

Untreated Hela extract lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

false

• WB

Supplier Data

Western blot - Anti-Histone H3 (acetyl K36) antibody [EPR16992] - BSA and Azide free (AB249945)

This data was developed using ab177179, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Histone H3 (acetyl K36) antibody [EPR16992] (<a href='/en-us/products/primary-antibodies/histone-h3-acetyl-k36-antibody-epr16992-ab177179'>ab177179</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 treated with Trichostatin A whole cell lysates at 10 µg

Lane 2:

Untreated NIH/3T3 whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

• PepArr

Supplier Data

Peptide Array - Anti-Histone H3 (acetyl K36) antibody [EPR16992] - BSA and Azide free (AB249945)

This data was developed using ab177179, the same antibody clone in a different buffer formulation.

ab177179 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.