Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)

Rabbit Recombinant Multiclonal H3 acetyl K4 + K9 + K14 + K18 + K23 + K27 antibody. Carrier free. Suitable for Dot, PepArr, IP, Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB and reacts with Synthetic peptide - Human, Human, Mouse, Rat samples.

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Images

Flow Cytometry (Intracellular) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (AB300642), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ChIP	Dot	PepArr	IP	Flow Cyt (Intra)	ICC/IF	IHC-Fr	IHC-P	WB
Human	Not recommended	Expected	Tested	Tested	Tested	Expected	Expected	Tested	Tested
Mouse	Not recommended	Expected	Expected	Tested	Tested	Tested	Tested	Tested	Tested
Rat	Not recommended	Expected	Expected	Expected	Expected	Tested	Tested	Tested	Tested
Synthetic peptide - Human	Not recommended	Tested	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat, Mouse, Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Target data

See full target information H3C1 acetyl K4 + K9 + K14 + K18 + K23 + K27

Function

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: RM1045
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab300642 is a carrier free version of Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

16 product images

Flow Cytometry (Intracellular) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
This data was developed using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) treated with 500ng/ml TSA for 4h (Red) / Untreated control (Green) cells labelling Histone H3(acetyl K4+K9+K14+K18+K23+K27) with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 at 1/500 dilution (0.1ug) (Red) and Green (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
This data was developed using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) treated with 500ng/ml TSA for 4h (Red) / Untreated control (Green) cells labelling Histone H3(acetyl K4+K9+K14+K18+K23+K27) with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 at 1/500 dilution (0.1ug) (Red) and Green (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Peptide Array - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
This data was developed using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641, the same antibody clone in a different buffer formulation. Peptide array analysis of Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 at 0.1ug/ml (0.1ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Fluo 647nm conjugated at 1:50,000 dilution.
All batches of Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including a full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
Western blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
All lanes: Western blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HeLa treated with /ml TSA for 4h whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Western blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
All lanes: Western blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 2: NIH/3T3 treated with /ml TSA for 4h whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Western blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
All lanes: Western blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641) at 1/1000 dilution
Lane 1: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 2: C6 treated with /ml TSA for 4h whole cell lysate 20ug at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
This data was developed using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H3(acetyl K4+K9+K14+K18+K23+K27) with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 at 1/2000 (0.239 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human colon. The section was incubated with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
This data was developed using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Histone H3(acetyl K4+K9+K14+K18+K23+K27) with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 at 1/2000 (0.239 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human tonsil. The section was incubated with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
This data was developed using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human Prostatic hype tissue labeling Histone H3(acetyl K4+K9+K14+K18+K23+K27) with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 at 1/2000 (0.239 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human prostatic hyperplasia. The section was incubated with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
This data was developed using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H3(acetyl K4+K9+K14+K18+K23+K27) with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 at 1/2000 (0.239 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on mouse colon. The section was incubated with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
This data was developed using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H3(acetyl K4+K9+K14+K18+K23+K27) with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 at 1/2000 (0.239 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on rat colon. The section was incubated with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
This data was developed using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon (fresh) tissue labeling Histone H3(acetyl K4+K9+K14+K18+K23+K27) with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 at 1/500 (0.954 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse colon is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
This data was developed using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat colon (fresh) tissue labeling Histone H3(acetyl K4+K9+K14+K18+K23+K27) with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 at 1/500 (0.954 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat colon is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
This data was developed using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Histone H3(acetyl K4+K9+K14+K18+K23+K27) with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 at 1/50 (9.54 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing increased nuclear staining in HeLa cells treated with 500 ng/mL TSA for 4 hours is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] - BSA and Azide free (ab300642)
This data was developed using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Histone H3(acetyl K4+K9+K14+K18+K23+K27) with Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 at 1/50 (9.54 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing increased nuclear staining in NIH/3T3 cells treated with 500 ng/mL TSA for 4 hours is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.
Dot Blot - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (BSA and Azide free) (ab300642)
This data was developed using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641, the same antibody clone in a different buffer formulation. Dot blot analysis of Histone H3(acetyl K4+K9+K14+K18+K23+K27) using Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] ab300641 at 1: 1000 dilution (0.477 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:10,000 dilution.

Lane 1: Histone H3 (acetyl K4) peptide
Lane 2: Histone H3 (acetyl K4) peptide
Lane 3: Histone H3 (acetyl K14) peptide
Lane 4: Histone H3 (acetyl K18) peptide
Lane 5: Histone H3 (acetyl K23) peptide
Lane 6: Histone H3 (acetyl K27) peptide
Lane 7: Histone H3 unmodified peptide

Exposure time: 40 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST