AB238307

Anti-Histone H3 (acetyl K56) antibody [RM179]

Name: Anti-Histone H3 (acetyl K56) antibody [RM179] (ab238307) | Abcam
Brand: Abcam Limited
SKU: AB238307
Price: 510 USD
Availability: InStock

(1 Review)

(3 Publications)

Rabbit Monoclonal H3 acetyl K56 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human H3C1 acetyl K56.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K56ac

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K56) antibody [RM179] (AB238307)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K56) antibody [RM179] (AB238307)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Histone H3 (acetyl K56) using ab238307 at 2 μg/ml in ICC/IF.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K56) antibody [RM179] (AB238307)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H3 (acetyl K56) using ab238307 (red) at 2 μg/ml in ICC/IF.

Actin filaments have been labeled with fluorescein phalloidin (green).``

ELISA - Anti-Histone H3 (acetyl K56) antibody [RM179] (AB238307)

ELISA

Supplier Data

ELISA - Anti-Histone H3 (acetyl K56) antibody [RM179] (AB238307)

ab238307 specifically reacts to Histone H3 acetylated at Lysine 56 (K56ac).

No cross reactivity with unmodified Lysine 56 (K56 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.``

Supplier Data

Western blot - Anti-Histone H3 (acetyl K56) antibody [RM179] (AB238307)

All lanes:

Western blot - Anti-Histone H3 (acetyl K56) antibody [RM179] (ab238307) at 1 µg/mL

Lane 1:

Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma cells treated with sodium butyrate

Lane 2:

Recombinant Histone H3.3

Predicted band size: 15 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM179

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, ELISA

applications

Immunogen

Synthetic Peptide within Human H3C1 acetyl K56. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

ab238307 reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with other acetylated Lysines in histone H3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-2 µg/mL", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from an animal origin–free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 acetyl K56

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cachexia, sarcopenia and muscle 16:e13734 PubMed39971710

2025

SIRT6 Ameliorates Cancer Cachexia-Associated Adipose Wasting by Suppressing TNFR2 Signalling in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Xu,Yida Wang,Fang Wang,Yannan Guo,Yu Ren,Vivien Low,Sungyun Cho,Qingfei Liu,Ying Qiu,Xue Li,Kang Yu,Zhongchi Li,Zhao Wang

Journal of nanobiotechnology 21:280 PubMed37598147

2023

Targeting macrophage M1 polarization suppression through PCAF inhibition alleviates autoimmune arthritis via synergistic NF-κB and H3K9Ac blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Jinteng Li,Feng Ye,Xiaojun Xu,Peitao Xu,Peng Wang,Guan Zheng,Guiwen Ye,Wenhui Yu,Zepeng Su,Jiajie Lin,Yunshu Che,Zhidong Liu,Pei Feng,Qian Cao,Dateng Li,Zhongyu Xie,Yanfeng Wu,Huiyong Shen

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21827 PubMed34383980

2021

Atypic SUMOylation of Nor1/NR4A3 regulates neural cell viability and redox sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Gagnon,Véronique Caron,Laurent Gyenizse,André Tremblay

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (acetyl K56) antibody [RM179]

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K56) antibody [RM179] (AB238307)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K56) antibody [RM179] (AB238307)

ELISA - Anti-Histone H3 (acetyl K56) antibody [RM179] (AB238307)

Western blot - Anti-Histone H3 (acetyl K56) antibody [RM179] (AB238307)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise