AB214731

Anti-Histone H3 (acetyl K79) antibody [RM156]

Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal H3 acetyl K79 antibody. Suitable for ChIP, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H3C1 acetyl K79.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K79ac

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

Immunofluorescent analysis of HeLa cells treated with sodium butyrat labeling Histone H3 (Acetyl-Lys79)` `with ab214731 at 2 μg/mL dilution (red); actin filaments have been labeled with fluorescein phalloidin (green).

ChIP - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ChIP

Supplier Data

ChIP - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using ab214731 at 5μg/mL dilution. Real-time PCR was performed using primers specific to the gene ``indicated.

ELISA - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ELISA

Supplier Data

ELISA - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

Sandwich ELISA labeling acetylated Histone H3 at Lys 79 in HeLa whole cell lysate, treated or untreated with Sodium Butyrate using ab214731 at 5 μg/mL dilution as the capture antibody and biotinylated anti-H3CT at 1 μg/mL dilution as the detection antibody.

ELISA

Supplier Data

ELISA - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ab214731 specifically reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 ``(K56ac), or Lysine 122 (K122) in Histone H3.

Supplier Data

Western blot - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

All lanes:

Western blot - Anti-Histone H3 (acetyl K79) antibody [RM156] (ab214731) at 1 µg/mL

Lane 1:

acid extracts from HeLa cells untreated

Lane 2:

acid extracts from HeLa cells treated with sodium butyrate

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM156

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, ELISA, ChIP

applications

Immunogen

Synthetic Peptide within Human H3C1 acetyl K79. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

ab214731 reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with other acetylated Lysines in Histone H3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-10 µg/mL", "ChIP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.2-1 µg/mL", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-2 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 acetyl K79

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 21:280 PubMed37598147

2023

Targeting macrophage M1 polarization suppression through PCAF inhibition alleviates autoimmune arthritis via synergistic NF-κB and H3K9Ac blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Jinteng Li,Feng Ye,Xiaojun Xu,Peitao Xu,Peng Wang,Guan Zheng,Guiwen Ye,Wenhui Yu,Zepeng Su,Jiajie Lin,Yunshu Che,Zhidong Liu,Pei Feng,Qian Cao,Dateng Li,Zhongyu Xie,Yanfeng Wu,Huiyong Shen

Disease markers 2021:1571421 PubMed34840626

2021

Characterization of Cancer Stem Cell Characteristics and Development of a Prognostic Stemness Index Cell-Related Signature in Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Feng,Yunxia Li,Ning Wen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (acetyl K79) antibody [RM156]

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ChIP - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ELISA - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

ELISA - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

Western blot - Anti-Histone H3 (acetyl K79) antibody [RM156] (AB214731)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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