AB272150

Anti-Histone H3 (acetyl K9) antibody

Name: Anti-Histone H3 (acetyl K9) antibody (ab272150) | Abcam
Brand: Abcam Limited
SKU: AB272150
Price: 640 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal H3 acetyl K9 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Mouse, Caenorhabditis elegans, Synthetic peptide samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H3C1 acetyl K9.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K9ac

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody (AB272150)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody (AB272150)

Immunofluorescence analysis of 0.5% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (acetyl K9) (green) with ab272150 at 1/100 dilution for 1 hour at room temperature, followed by FITC secondary antibody at 1/10,000 for 45 minutes at room temperature.

ChIP - Anti-Histone H3 (acetyl K9) antibody (AB272150)

ChIP

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ChIP - Anti-Histone H3 (acetyl K9) antibody (AB272150)

Chromatin Immunoprecipitation analysis using ab272150. Chromatin from one million formaldehyde cross-linked HeLa (human epithelial cell line from cervix adenocarcinoma) cells and 2 μg of ab272150 was used to IP DNA from fixed HeLa cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).

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Western blot - Anti-Histone H3 (acetyl K9) antibody (AB272150)

All lanes:

Western blot - Anti-Histone H3 (acetyl K9) antibody (ab272150) at 1/500 dilution

All lanes:

NIH/3T3 (mouse embryo fibroblast cell line) histone preps at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Unknown

Western blot - Anti-Histone H3 (acetyl K9) antibody (AB272150)

All lanes:

Western blot - Anti-Histone H3 (acetyl K9) antibody (ab272150) at 1/500 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) histone preps at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Unknown

Western blot - Anti-Histone H3 (acetyl K9) antibody (AB272150)

All lanes:

Western blot - Anti-Histone H3 (acetyl K9) antibody (ab272150) at 1/500 dilution

All lanes:

C.elegans embryo lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Unknown

Dot Blot - Anti-Histone H3 (acetyl K9) antibody (AB272150)

Dot Blot of ab272150.

Lane 1 : K9.

Lane 2 : K9 Me1.

Lane 3 : K9 Me2.

Lane 4 : K9 Me3.

Lane 5 : K9ac.

Load : 1, 10, and 100 picomoles of peptide. Primary antibody ab272150 at 1/1000 for 45 minutes at 4°C. Dylight™488 rabbit secondary antibody at 1/10000 for 45 minutes at room temperature.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Caenorhabditis elegans

Applications

Dot, WB, ChIP, ICC/IF

applications

Immunogen

Synthetic Peptide within Human H3C1 acetyl K9. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-5 µg/cells for 6 µg/cells", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Anti-Histone H3 [ac Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 acetyl K9

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer & metabolism 10:22 PubMed36474242

2022

Tadalafil increases the antitumor activity of 5-FU through inhibiting PRMT5-mediated glycolysis and cell proliferation in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Shen,Pan Zhao,Kewei Dong,Jiajia Wang,Huichen Li,Mengyang Li,Ruikai Li,Suning Chen,Yuxia Shen,Zhiyu Liu,Mianjiao Xie,Peng Shen,Jian Zhang

Scientific reports 11:13236 PubMed34168220

2021

Fms-like tyrosine kinase 3-internal tandem duplications epigenetically activates checkpoint kinase 1 in acute myeloid leukemia cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yudong Zhang,Lingli Yuan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (acetyl K9) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody (AB272150)

ChIP - Anti-Histone H3 (acetyl K9) antibody (AB272150)

Western blot - Anti-Histone H3 (acetyl K9) antibody (AB272150)

Western blot - Anti-Histone H3 (acetyl K9) antibody (AB272150)

Western blot - Anti-Histone H3 (acetyl K9) antibody (AB272150)

Dot Blot - Anti-Histone H3 (acetyl K9) antibody (AB272150)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise