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AB10812

Anti-Histone H3 (acetyl K9) antibody - ChIP Grade

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(25 Reviews)

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(333 Publications)

Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab10812) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ChIP. Suitable for Arabidopsis thaliana, Cow, Human.

- Over 290 publications
- Trusted since 2005

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K9ac

6 Images
Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (AB10812)
  • WB

Project4020****

Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (AB10812)

All lanes:

Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab10812) at 1 µg/mL

Lanes 1, 2, 3, 6 and 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H3 peptide - acetyl K14

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (acetyl K18) peptide (<a href='/en-us/products/unavailable/human-histone-h3-acetyl-k18-peptide-ab24003'>ab24003</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 30s

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (AB10812)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (AB10812)

ab10812 staining histone H3 (acetyl K9) in A549 cells treated with scriptaid (ab120883), by ICC/IF. Increase in histone H3 (acetyl K9) expression correlates with increased concentration of scriptaid, as described in literature.
The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120883 (scriptaid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab10812 (0.1 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A anti-rabbit DyLight 488 secondary antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (AB10812)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (AB10812)

IHC image of Histone H3 (acetyl K9) staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10812, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (AB10812)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (AB10812)

ab10812 staining Histone H3 (acetyl K9) in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab10812 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

ChIP - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (AB10812)
  • ChIP

Unknown

ChIP - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (AB10812)

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab10812 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (AB10812)
  • ICC/IF

AbReview17719****

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (AB10812)

ab10812 staining Histone H3 (acetyl K9) in the root tips of Arabidopsis thaliana by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 2% paraformaldehyde for 30 minutes at room temperature. Blocking and permeabilization was carried with 4% BSA solution containing 0,5% Triton X-100 in PBS at room temperature for 45 minutes. Slides were washed in PBS and incubated with primary antibody at a 1/200 dilution in 1% PBS for 1 hour at 37°C. Slides were washed in PBS and incubated with the secondary antibody ab6639 (Goat anti-rabbit Cy3 ® (H&L)) at a 1/500 dilution in 1% BSA in PBS for 1 hour at 37°C. Slides were counterstained with DAPI (2μg/mL) for 10 minutes at room temperature.Left image : DAPI stained interphase nucleus with prominent chromocentersMiddle image : Distribution of Histone H3 (acetyl K9) in the nucleus (arrows indicate absence of signal from chromocenters)Right image : Merged image

This image was kindly supplied by Ms Vedrana Vicic by Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Cow, Human, Arabidopsis thaliana

Applications

ChIP, IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Specific for acetyl K9, but cross-reacts slightly with K27.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-4 µg for 25 µg chromatin", "ChIP-species-notes": "<p>Use GAPDH ChIP primer pair <a href='/en-us/products/reagents/gapdh-positive-control-chip-primer-pair-ab267832'>ab267832</a> as positive control.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Arabidopsis thaliana": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Caenorhabditis elegans": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Drosophila melanogaster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rice": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Saccharomyces cerevisiae": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab10812) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in Arabidopsis thaliana, Cow, Human samples.

What is the molecular weight of Histone H3?
Anti-Histone H3 (acetyl K9) - ChIP Grade (ab10812) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa.

Trusted by the scientific community
Anti-Histone H3 (acetyl K9) - ChIP Grade (ab10812) was first used in a scientific publication in 2005 and has been cited over 290 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H3 (acetyl K9) - ChIP Grade (ab10812) has over 25 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 acetyl K9
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Publications (333)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eady5876 PubMed41004569

2025

The polymerase-associated factor 1 complex modulates the growth-defense tradeoff in .

Applications

Unspecified application

Species

Unspecified reactive species

Yuyu Guo,Cunliang Li,Yao Liu,Hanyi Mao,Yin Zhang,Pascal Genschik,Lili Wang,Shunping Yan

Nucleic acids research 53: PubMed40737091

2025

A U3 snoRNA is required for the regulation of chromatin dynamics and antiviral response in Drosophila melanogaster.

Applications

Unspecified application

Species

Unspecified reactive species

Shruti Jain,Jordi Planells,Isabel Regadas,Donal Barrett,Anne von Euler,Indranil Sinha,Bo Gustav Lindberg,Jenny C Hesson,Patrycja Swacha,Nelson O Gekara,Vicent Pelechano,Ylva Engström,Mattias Mannervik,Neus Visa

Nature communications 16:5226 PubMed40473600

2025

SWI/SNF ATPase silenced HLF potentiates lung metastasis in solid cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Zhou,Austin Hepperla,Jeremy M Simon,Kangsan Kim,Qing Hu,Chuanhai Zhang,Lei Dong,Lianxin Hu,Cheng Zhang,Chengheng Liao,Alice Fang,Yayoi Adachi,Haoyong Fu,Tao Wang,Qian Liang,Fangzhou Zhao,Hongyi Liu,Masashi Takeda,Jun Fang,Hua Zhong,Peter Ly,Lu Wang,Payal Kapur,Lin Xu,Liwei Jia,Srinivas Malladi,James Brugarolas,M Celeste Simon,Bo Li,Qing Zhang

Nature communications 16:4697 PubMed40393969

2025

ACSS2 mediates an epigenetic pathway to regulate β-cell adaptation during gestation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Shuang He,Xi Wang,Xin Wang,Mao-Yang He,Xin-Xin Yu,Cheng-Ran Xu

The EMBO journal 44:3056-3084 PubMed40281356

2025

Altering metabolism programs cell identity via NAD-dependent deacetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Robert A Bone,Molly P Lowndes,Silvia Raineri,Alba R Riveiro,Sarah L Lundregan,Morten Dall,Karolina Sulek,Jose A H Romero,Luna Malzard,Sandra Koigi,Indra J Heckenbach,Victor Solis-Mezarino,Moritz Völker-Albert,Catherine G Vasilopoulou,Florian Meier,Ala Trusina,Matthias Mann,Michael L Nielsen,Jonas T Treebak,Joshua M Brickman

Nature communications 16:1500 PubMed39929795

2025

Simultaneous profiling of chromatin-associated RNA at targeted DNA loci and RNA-RNA Interactions through TaDRIM-seq.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Ding,Guoting Chen,Shiping Luan,Runxin Gao,Yudong Fan,Ying Zhang,Xiaoting Wang,Guoliang Li,Mohamed F Foda,Jiapei Yan,Xingwang Li

Cellular and molecular neurobiology 45:19 PubMed39923205

2025

Organochlorine Pesticides and Epigenetic Alterations in Brain Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh Yousefi,Gholamreza Asadikaram,Saeid Karamouzian,Moslem Abolhassani,Hossein Pourghadamyari,Vahid Moazed

Plant biotechnology journal 23:900-910 PubMed39676575

2024

The OsNL1-OsTOPLESS2-OsMOC1/3 pathway regulates high-order tiller outgrowth in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Liu,Feng Zhang,Ziqi Xun,Jiale Shao,Wenfan Luo,Xiaokang Jiang,Jiachang Wang,Jian Wang,Shuai Li,Qibing Lin,Yulong Ren,Huixian Zhao,Zhijun Cheng,Jianmin Wan

The New phytologist 245:1589-1607 PubMed39639540

2024

Epigenetic regulation of lignin biosynthesis in wood formation.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Ma,Liwei Su,Wen Zhang,Yi Sun,Danning Li,Shuang Li,Ying-Chung Jimmy Lin,Chenguang Zhou,Wei Li

PloS one 19:e0314329 PubMed39637191

2024

LARP7 is required for sex chromosome silencing during meiosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yukiko Tando,Atsuto Nonomura,Yumi Ito-Matsuoka,Asuka Takehara,Daiji Okamura,Yohei Hayashi,Yasuhisa Matsui
View all publications
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