Anti-Histone H3 (acetyl K9) antibody [EPR21217]

• WB

Lab

Western blot - Anti-Histone H3 (acetyl K9) antibody [EPR21217] (AB213280)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213280 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

All lanes:

Western blot - Anti-Histone H3 (acetyl K9) antibody [EPR21217] (ab213280) at 1 µg/mL

Lane 1:

HeLa whole cell lysate at 10 µg

Lane 2:

HeLa Triton-nuclear lysate at 10 µg

Lane 3:

HeLa Triton-nuclear TSA (-) lysate at 10 µg

Lane 4:

HeLa Triton-nuclear TSA (+) lysate at 10 µg

Lane 5:

NIH3T3 Triton-nuclear lysate at 10 µg

Lane 6:

NIH3T3 Triton-nuclear TSA (-) lysate at 10 µg

Lane 7:

NIH3T3 Triton-nuclear TSA (+) lysate at 10 µg

Secondary

All lanes:

anti-rabbit antibody conjugated to HRP at 1/50000 dilution

Predicted band size: 15 kDa

true

Exposure time: 4min

• PepArr

Lab

Peptide Array - Anti-Histone H3 (acetyl K9) antibody [EPR21217] (AB213280)

ab213280 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). The concentration of ab213280 was 1/5000 dilution, the secondary used was Goat Anti-Rabbit IgG, (H+L), Fluo 647nm conjugated at 1/50000 dilution. Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity. The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.