Anti-Histone H3 (acetyl K9) antibody [EPR21239]

• PepArr

Lab

Peptide Array - Anti-Histone H3 (acetyl K9) antibody [EPR21239] (AB241151)

ab241151 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). The concentration of ab241151 was 1/2500 dilution, the secondary used was Goat Anti-Rabbit IgG, (H+L), Fluo 647nm conjugated at 1/50000 dilution. Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity. The complete dataset, including a full list of all peptides and information on the position of each peptide in the diagram, can be downloaded from the Support and downloads tab.

• WB

Lab

Western blot - Anti-Histone H3 (acetyl K9) antibody [EPR21239] (AB241151)

This blot was produced using a 4-20% Criterion TGX gel in 1X SDS buffer. The gel was run at 180V for 40 minutes before being transferred onto a Nitrocellulose membrane at 25V for 7 minutes using the Trans-Blot turbo transfer system. The membrane was then blocked for an hour using 3% milk before being incubated with ab241151 overnight at 4°C. Antibody binding was detected using a goat anti-Rabbit antibody conjugated to HRP, and visualised using Bio-Rad Clarity Western ECL substrate

All lanes:

Western blot - Anti-Histone H3 (acetyl K9) antibody [EPR21239] (ab241151) at 1 µg/mL

Lane 1:

HeLa triton nuclear lysate at 10 µg

Lane 2:

NIH3T3 triton nuclear lysate at 10 µg

Secondary

All lanes:

HRP conjugated Goat Anti-Human IgG (H+L) at 1/5000 dilution

Predicted band size: 15 kDa

true

Exposure time: 5s

• WB

Lab

Western blot - Anti-Histone H3 (acetyl K9) antibody [EPR21239] (AB241151)

This blot was produced using a 4-20% Criterion TGX gel in 1X SDS buffer. The gel was run at 180V for 40 minutes before being transferred onto a Nitrocellulose membrane at 25V for 7 minutes using the Trans-Blot turbo transfer system. The membrane was then blocked for an hour using 3% milk before being incubated with ab241151 overnight at 4°C. Antibody binding was detected using a Goat anti-Rabbit antibody conjugated to HRP, and visualised using Bio-Rad Clarity Western ECL substrate.

All lanes:

Western blot - Anti-Histone H3 (acetyl K9) antibody [EPR21239] (ab241151) at 1 µg/mL

Lane 1:

HeLa Whole Cell Lysate at 10 µg

Lane 2:

HeLa Histone Preparation Nuclear Lysate at 10 µg

Lane 3:

NIH3T3 Histone Preparation Nuclear Lysate at 10 µg

Lane 4:

Purified Core Histones from Sodium Butyrate Treated HeLa Cells at 10 µg

Lane 5:

Purified Core Histones from Untreated HeLa Cells at 10 µg

Secondary

All lanes:

HRP conjugated Goat Anti-Rabbit IgG (H+L) at 1/5000 dilution

Predicted band size: 15 kDa

true

Exposure time: 2s

• PepArr

Lab

Peptide Array - Anti-Histone H3 (acetyl K9) antibody [EPR21239] (AB241151)

All batches of ab241151 are tested in Peptide Array against peptides to different Histone H2, H3 and H4 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - acetyl K9 peptide (ab16635), indicating that this antibody specifically recognises the Histone H3 - acetyl K9 modification.+-----------------------------------------------------------------------------------------------------+---------+ | ab16635 - H3 K9ac | |