AB218553

Anti-Histone H3 (acetyl K9) antibody [RM161]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal H3 acetyl K9 antibody. Suitable for ICC, ChIP, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C1 acetyl K9.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K9ac

6 Images

Supplier Data

Immunocytochemistry - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Immunocytochemical analysis of HepG2 cells labeling Histone H3 (acetyl K9) using ab218553 at 1 μg/mL.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Immunocytochemistry analysis of HeLa cells treated with sodium butyrate labeling Histone H3 (acetyl K9) using ab218553 at 2 μg/mL (red). Actin filaments have been labeled with fluorescein phalloidin (green).

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ELISA

Supplier Data

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using ab218553 at 1 ug/mL as the capture antibody and biotinylated anti-H3K9ac as the detection antibody.

ChIP - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ChIP

Supplier Data

ChIP - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ChIP performed on HeLa cells using ab218553 at 5ug. Real-time PCR was performed using primers specific to the gene indicated.

Supplier Data

Western blot - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

All lanes:

Western blot - Anti-Histone H3 (acetyl K9) antibody [RM161] (ab218553) at 0.25 µg/mL

Lane 1:

HeLa cells untreated

Lane 2:

HeLa cells treated with sodium butyrate

Predicted band size: 15 kDa

false

ELISA

Supplier Data

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ab218553 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM161

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, ELISA, ICC/IF, ChIP, WB

applications

Immunogen

Synthetic Peptide within Human H3C1 acetyl K9. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

Reacts to Histone H3 (acetyl K9). No cross reactivity with other acetylated Lysines in histone H3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.5-2 µg/mL", "ICC-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-10 µg/mL", "ChIP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.2-1 µg/mL", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-2 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 acetyl K9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 99:e0198024 PubMed39976465

2025

Zika virus inhibits cell death by inhibiting the expression of NLRP3 and A20.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,Changyang Zhu,Yang Meng,Linliang Zhang,Cong Liu,Yali Qin,Mingzhou Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (acetyl K9) antibody [RM161]

Immunocytochemistry - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ChIP - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Western blot - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (AB218553)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise