Rabbit Polyclonal H3 acetyl K9 + phospho S10 antibody. Suitable for Dot, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10.
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Publications
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- Frontiers in immunology 12:8044082021Tryptase Regulates the Epigenetic Modification of Core Histones in Mast Cell Leukemia Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSultan Alanazi,Fabio Rabelo Melo,Gunnar PejlerPubMed 34925389
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10. The exact immunogen used to generate this antibody is proprietary information. Database link Q71DI3
Reactivity data
Select an application| Dot | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
HIST2H3A, H3C14, H3F2, H3FM, HIST2H3C, H3C13, HIST2H3D, H3C15, Histone H3.2, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, Histone H3/m, Histone H3/o
Recommended products
Rabbit Polyclonal H3 acetyl K9 + phospho S10 antibody. Suitable for Dot, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10. The exact immunogen used to generate this antibody is proprietary information. Database link Q71DI3
- Purification technique
- Affinity purification
- Concentration
- Purification notes
Anti-Histone H3 K9Ac/pS10 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab272166)
Block: 5% BLOTTO overnight at 4°C.
All lanes: Western blot - Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab272166) at 1/400 dilution
All lanes: NIH/3T3 cell lysate at 35 µg
Predicted band size: 15 kDa
Dot Blot - Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab272166)
Dot Blot analysis of Histone H3 (acetyl K9, phospho S10) using ab272166 at 1/1000 dilution.
Lane 1: K9-Ac/pS10 unmodified.
Lane 2: pS10.
Lane 3: K9-Me1.
Lane 4: K9-Me2.
Lane 5: K9-Me3.
Lane 6: K9-Ac peptides.
Load: 1, 10, and 100 picomoles of peptide.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab272166)
Immunofluorescent analysis of HeLa cells labeling Histone H3 (acetyl K9, phospho S10) using ab272166 at 1/10 dilution (Green). The nuclear counterstain is DAPI (Blue). Alpha tubulin is shown in red.
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