Mouse Monoclonal H3 antibody. Suitable for ChIP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human H3C1 conjugated to Keyhole Limpet Haemocyanin.
Images
![ChIP - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (AB195277), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-1b1b2-nuclear-marker-and-chip-grade-ab195277--chip-img109242.jpg/_jcr_content/renditions/webp-475.webp "ChIP - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (AB195277)")
![Western blot - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (AB195277), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-1b1b2-nuclear-marker-and-chip-grade-ab195277--western-blot-img120786.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (AB195277)")
![Western blot - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (AB195277), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-1b1b2-nuclear-marker-and-chip-grade-ab195277--western-blot-img3180.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (AB195277)")
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [1B1B2] - ChIP Grade (AB195277), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-1b1b2-nuclear-marker-and-chip-grade-ab195277--immunocytochemistry-immunofluorescence-img56584.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [1B1B2] - ChIP Grade (AB195277)")
Publications
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- iScience 25:1049782022Confined migration induces heterochromatin formation and alters chromatin accessibility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChieh-Ren Hsia,Jawuanna McAllister,Ovais Hasan,Julius Judd,Seoyeon Lee,Richa Agrawal,Chao-Yuan Chang,Paul Soloway,Jan LammerdingPubMed 36117991
- PLoS genetics 18:e10102232022Inhibition of histone H3-H4 chaperone pathways rescues C. elegans sterility by H2B loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuixue Zhao,Zhiwen Zhu,Ruxu Geng,Xuguang Jiang,Wei Li,Guangshuo OuPubMed 35679337
- Nature communications 13:13032022The arginine methyltransferase PRMT7 promotes extravasation of monocytes resulting in tissue injury in COPD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGizem Günes Günsel,Thomas M Conlon,Aicha Jeridi,Rinho Kim,Zeynep Ertüz,Niklas J Lang,Meshal Ansari,Mariia Novikova,Dongsheng Jiang,Maximilian Strunz,Mariia Gaianova,Christine Hollauer,Christina Gabriel,Ilias Angelidis,Sebastian Doll,Jeanine C Pestoni,Stephanie L Edelmann,Marlene Sophia Kohlhepp,Adrien Guillot,Kevin Bassler,Hannelore P Van Eeckhoutte,Özgecan Kayalar,Nur Konyalilar,Tamara Kanashova,Sophie Rodius,Carolina Ballester-López,Carlos M Genes Robles,Natalia Smirnova,Markus Rehberg,Charu Agarwal,Ioanna Krikki,Benoit Piavaux,Stijn E Verleden,Bart Vanaudenaerde,Melanie Königshoff,Gunnar Dittmar,Ken R Bracke,Joachim L Schultze,Henrik Watz,Oliver Eickelberg,Tobias Stoeger,Gerald Burgstaller,Frank Tacke,Vigo Heissmeyer,Yuval Rinkevich,Hasan Bayram,Herbert B Schiller,Marcus Conrad,Robert Schneider,Ali Önder YildirimPubMed 35288557
- Signal transduction and targeted therapy 6:3752021The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXujun Liu,Wenzhe Si,Lin He,Jianguo Yang,Yani Peng,Jie Ren,Xiaoping Liu,Tong Jin,Huajing Yu,Zihan Zhang,Xiao Cheng,Wenting Zhang,Lu Xia,Yunchao Huang,Yue Wang,Shumeng Liu,Lin Shan,Yu Zhang,Xiaohan Yang,Haixia Li,Jing Liang,Luyang Sun,Yongfeng ShangPubMed 34728602
- Nature microbiology 6:257-2692020The histone demethylase KDM6B fine-tunes the host response to Streptococcus pneumoniae.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael G Connor,Tiphaine M N Camarasa,Emma Patey,Orhan Rasid,Laura Barrio,Caroline M Weight,Daniel P Miller,Robert S Heyderman,Richard J Lamont,Jost Enninga,Melanie A HamonPubMed 33349663
- Current biology : CB 31:753-765.e62020Nuclear Deformation Causes DNA Damage by Increasing Replication Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPragya Shah,Chad M Hobson,Svea Cheng,Marshall J Colville,Matthew J Paszek,Richard Superfine,Jan LammerdingPubMed 33326770
- Nature :2020Cytoplasmic control of intranuclear polarity by human cytomegalovirus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDean J Procter et. al.PubMed 32908309
- The American journal of pathology 190:2267-22812020Pyruvate Kinase M2 Tetramerization Protects against Hepatic Stellate Cell Activation and Liver Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDandan Zheng,Yuchuan Jiang,Chen Qu,Hui Yuan,Kaishun Hu,Lu He,Peng Chen,Jinying Li,Mengxian Tu,Lehang Lin,Hengxing Chen,Zelong Lin,Wenyu Lin,Jun Fan,Guohua Cheng,Jian HongPubMed 32805235
- Clinical and experimental immunology 199:1-82019Neutrophil proteases degrade autoepitopes of NET-associated proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC M de Bont,N Eerden,W C Boelens,G J M PruijnPubMed 31661552
- Arthritis & rheumatology (Hoboken, N.J.) 71:941-9512019Identification of Novel Autoantibodies Associated With Psoriatic Arthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulin Yuan,Jingyi Qiu,Zuan-Tao Lin,Wen Li,Christopher Haley,Uyen Ngoc Mui,Jing Ning,Stephen K Tyring,Tianfu WuPubMed 30618213
Key facts
- Isotype
- IgG3
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human H3C1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P68431
Reactivity data
Select an application| ChIP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 1-2μg per ChIP |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Recommended products
Mouse Monoclonal H3 antibody. Suitable for ChIP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human H3C1 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG3
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human H3C1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P68431
- Clone number
- 1B1B2
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab195277 can be used as a loading control for ChIP and Western blot experiments
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![ChIP - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (ab195277), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-1b1b2-nuclear-marker-and-chip-grade-ab195277--chip-img109242.jpg "ChIP - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (ab195277)")
ChIP - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (ab195277)
ChIP assays were performed using human HeLa cells, ab195277 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and EIF4A2 genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
![Western blot - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (ab195277), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-1b1b2-nuclear-marker-and-chip-grade-ab195277--western-blot-img120786.jpg "Western blot - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (ab195277)")
Western blot - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (ab195277)
Histone H3 Western blot staining using mouse Anti-Histone H3 antibody
All lanes: Western blot - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (ab195277) at 1/5000 dilution
Lane 1: HeLa cell lysates at 40 µg
Lane 2: Recombinant histone H3 at 1 µg
Lane 3: Recombinant histone H4 at 1 µg
Predicted band size: 15 kDa
![Western blot - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (ab195277), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-1b1b2-nuclear-marker-and-chip-grade-ab195277--western-blot-img3180.jpg "Western blot - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (ab195277)")
Western blot - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (ab195277)
Histone H3 Western blot staining using mouse Anti-Histone H3 antibody
The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.
All lanes: Western blot - Anti-Histone H3 antibody [1B1B2] - Nuclear Marker and ChIP Grade (ab195277) at 1/1000 dilution
Lane 1: HeLa whole cell extract at 30 µg
Lane 2: K562 whole cell extract at 30 µg
Lane 3: MCF7 whole cell extract at 30 µg
Lane 4: U2OS whole cell extract at 30 µg
Lane 5: HepG2 whole cell extract at 30 µg
Lane 6: Jurkat whole cell extract at 30 µg
Lane 7: NIH 3T3 whole cell extract at 30 µg
Lane 8: E14Tg2a Mouse ES whole cell extract at 30 µg
Predicted band size: 15 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-1b1b2-nuclear-marker-and-chip-grade-ab195277--immunocytochemistry-immunofluorescence-img56584.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277)
Immunofluorescent analysis of HeLa cells labeling Histone H3 with ab195277 at 1/500 dilution. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
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