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Rabbit Recombinant Monoclonal H3 antibody. Suitable for PepArr, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Synthetic peptide, Mouse, Rat, Human samples. Cited in 23 publications.


Images

Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (AB201456), expandable thumbnail
  • Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (AB201456), expandable thumbnail
  • Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (AB201456), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [EPR17785] (AB201456), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [EPR17785] (AB201456), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPPepArrChIPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Not recommended
Expected
Not recommended
Tested
Tested
Tested
Tested
Mouse
Not recommended
Expected
Not recommended
Tested
Expected
Expected
Tested
Rat
Not recommended
Expected
Not recommended
Tested
Expected
Expected
Tested
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

We've tested ab201456 twice in ChIP and it does not work in our hands. We would recommend ab176842 for use in ChIP experiments.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

We've tested ab201456 twice in ChIP and it does not work in our hands. We would recommend ab176842 for use in ChIP experiments.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

We've tested ab201456 twice in ChIP and it does not work in our hands. We would recommend ab176842 for use in ChIP experiments.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

-

Species
Rat
Dilution info
1/2000
Notes

-

Species
Human
Dilution info
1/2000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/800
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal H3 antibody. Suitable for PepArr, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Synthetic peptide, Mouse, Rat, Human samples. Cited in 23 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17785
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (ab201456), expandable thumbnail

    Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (ab201456)

    Histone H3 Western blot staining using rabbit Anti-Histone H3 antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (ab201456) at 1/2000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

    Lane 2: HEK293 (Human embryonic kidney) cell lysate at 20 µg

    Lane 3: A375 (Human malignant melanoma) cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

    Exposure time: 15s

  • Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (ab201456), expandable thumbnail

    Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (ab201456)

    Histone H3 Western blot staining of Human fetal brain lysate using rabbit Anti-Histone H3 antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (ab201456) at 1/2000 dilution

    All lanes: Human fetal brain lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

    Exposure time: 3min

  • Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (ab201456), expandable thumbnail

    Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (ab201456)

    Histone H3 Western blot staining using rabbit Anti-Histone H3 antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (ab201456) at 1/2000 dilution

    Lane 1: Mouse kidney lysate at 10 µg

    Lane 2: Rat brain lysate at 10 µg

    Lane 3: NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

    Exposure time: 1min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [EPR17785] (ab201456), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [EPR17785] (ab201456)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Histone H3 with ab201456 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.

    Nuclear staining on Human cervix carcinoma tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [EPR17785] (ab201456), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [EPR17785] (ab201456)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Histone H3 with ab201456 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.

    Nuclear staining on Human kidney tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [EPR17785] (ab201456), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [EPR17785] (ab201456)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H3 with ab201456 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.

    Nuclear staining on mouse colon tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [EPR17785] (ab201456), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 antibody [EPR17785] (ab201456)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Histone H3 with ab201456 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.

    Nuclear staining on rat stomach tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [EPR17785] (ab201456), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [EPR17785] (ab201456)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton-X100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H3 with ab201456 at 1/800 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/500 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is stained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/500 dilution (red).
    -ve control 1: ab201456 at 1/800 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/500 dilution.

  • Flow Cytometry (Intracellular) - Anti-Histone H3 antibody [EPR17785] (ab201456), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Histone H3 antibody [EPR17785] (ab201456)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H3with ab201456 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Peptide Array - Anti-Histone H3 antibody [EPR17785] (ab201456), expandable thumbnail

    Peptide Array - Anti-Histone H3 antibody [EPR17785] (ab201456)

    Peptide array analysis of ab201456 at 1/10000 (0.02 ug/ml) dilution followed by a Goat Anti-Rabbit IgG, (H+L), Fluo 647nm conjugated at 1/50000 dilution.

    Blocking buffer: 5% BSA in TBST.

    ab201456 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including a full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

  • Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (ab201456), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-Histone H3 antibody [EPR17785] - Nuclear Marker (ab201456)

    Histone H3 Western blot staining using rabbit Anti-Histone H3 antibody

    Histone H3 western blot using anti-Histone H3 antibody [EPR17785] ab201456. Publication image and figure legend from Bitar, M. S., Nader, J., et al., 2018, Oxid Med Cell Longev, PubMed 30510624.


    ab201456 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab201456 please see the product overview.

    Diabetes alters key signaling molecules involved in skeletal muscle mass regulation. (a) Representative immunoblots and quantifications of phosphorylated Akt, S6, and 4E-BP1 protein levels in muscles of control and GK diabetic rats. (b) Total phosphorylated levels of FoxO1 and its nuclear localization relative to GAPDH and histone, respectively, were analyzed by Western blot. (c) Expression of MuRF1 and Atrogin 1 mRNAs relative to GAPDH was examined by real-time PCR. (d) Expression of miRNA 486 relative to U6 was determined using real-time PCR (e) PTEN protein level (e) and activity (f) were measured as described in Materials and Methods. (f) Key regulators of myogenesis including IGF-1 and PAX7 (g) were assessed in muscle using real-time RT-PCR-based technique. Abbreviation: C: control; D: diabetic. Values are means ± SEM for at least 6 animals/group. ∗Significantly different from corresponding control values at P ≤ 0.05.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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