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Rabbit Recombinant Monoclonal H3 antibody. Suitable for ChIP, WB, PepArr and reacts with Human, Mouse, Cow, Synthetic peptide - Human samples. Cited in 2 publications.

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Images

ChIP - Anti-Histone H3 antibody [EPR21228] - Nuclear Marker (AB213257), expandable thumbnail
  • Western blot - Anti-Histone H3 antibody [EPR21228] - Nuclear Marker (AB213257), expandable thumbnail
  • Peptide Array - Anti-Histone H3 antibody [EPR21228] - Nuclear Marker (AB213257), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPWBPepArr
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Cow
Expected
Tested
Expected
Synthetic peptide - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
5 µg chromatin for 25 µg chromatin
Notes

-

Expected
Expected

Species
Mouse, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
1 µg/mL
Notes

-

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Species
Human
Dilution info
1 µg/mL
Notes

-

Species
Cow
Dilution info
1 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal H3 antibody. Suitable for ChIP, WB, PepArr and reacts with Human, Mouse, Cow, Synthetic peptide - Human samples. Cited in 2 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21228
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • ChIP - Anti-Histone H3 antibody [EPR21228] - Nuclear Marker (ab213257), expandable thumbnail

    ChIP - Anti-Histone H3 antibody [EPR21228] - Nuclear Marker (ab213257)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min.
    The ChIP was performed with 25μg of chromatin, 5μg of ab213257 (red), and 20 μl of Protein A/G sepharose beads. No antibody was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).

  • Western blot - Anti-Histone H3 antibody [EPR21228] - Nuclear Marker (ab213257), expandable thumbnail

    Western blot - Anti-Histone H3 antibody [EPR21228] - Nuclear Marker (ab213257)

    Histone H3 Western blot staining using rabbit Anti-Histone H3 antibody

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 40 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213257 overnight at 4°C. Antibody binding was detected using a goat anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - Anti-Histone H3 antibody [EPR21228] - Nuclear Marker (ab213257) at 1 µg/mL

    Lane 1: Calf Thymus Histone lysate at 0.5 µg

    Lane 2: Hela whole cell lysate at 20 µg

    Lane 3: HeLa histone preparation nuclear lysate at 10 µg

    Lane 4: NIH3T3 whole cell lysate at 10 µg

    Lane 5: NIH3T3 histone preparation nuclear lysate at 10 µg

    Lane 6: Histone H2B recombinant protein at 0.1 µg

    Lane 7: Histone H3.1 recombinant protein at 0.1 µg

    Secondary

    All lanes: HRP conjugated Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa

    Exposure time: 12min

  • Peptide Array - Anti-Histone H3 antibody [EPR21228] - Nuclear Marker (ab213257), expandable thumbnail

    Peptide Array - Anti-Histone H3 antibody [EPR21228] - Nuclear Marker (ab213257)

    ab213257 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). The concentration of ab213257 was 1/500 dilution, the secondary used was Goat Anti-Rabbit IgG, (H+L), Fluo 647nm conjugated at 1/50000 dilution.
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including a full list of all peptides and information on the position of each peptide in the diagram, can be downloaded from the Support and downloads tab.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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