Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) is a mouse monoclonal antibody detecting Histone H3 in Western Blot, IP, ChIP. Suitable for Human, Mouse, Rat.
- Over 50 publications
- Trusted since 2005
Images
![Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (AB24834), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-mabcam-24834-nuclear-loading-control-and-chip-grade-ab24834--western-blot-img53029.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (AB24834)")
![ChIP - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (AB24834), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-mabcam-24834-nuclear-loading-control-and-chip-grade-ab24834--chip-img16667.jpg/_jcr_content/renditions/webp-475.webp "ChIP - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (AB24834)")
![Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (AB24834), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-mabcam-24834-nuclear-loading-control-and-chip-grade-ab24834--western-blot-img99045.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (AB24834)")
Publications
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- Nature genetics 56:100-1112023The BAF chromatin remodeler synergizes with RNA polymerase II and transcription factors to evict nucleosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandipan Brahma,Steven HenikoffPubMed 38049663
- Nature communications 14:28612023ADAR1-mediated RNA editing of SCD1 drives drug resistance and self-renewal in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTin-Lok Wong,Jia-Jian Loh,Shixun Lu,Helen H N Yan,Hoi Cheong Siu,Ren Xi,Dessy Chan,Max J F Kam,Lei Zhou,Man Tong,John A Copland,Leilei Chen,Jing-Ping Yun,Suet Yi Leung,Stephanie MaPubMed 37208334
- Pathogens (Basel, Switzerland) 11:2022[PRION] States Are Associated with Specific Histone H3 Post-Translational Modification Changes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamantha N Cobos,Chaim Janani,Gabriel Cruz,Navin Rana,Elizaveta Son,Rania Frederic,Jailene Paredes Casado,Maliha Khan,Seth A Bennett,Mariana P TorrentePubMed 36558770
- Genes, brain, and behavior 22:e128302022Neuropathic pain as a trigger for histone modifications in limbic circuitry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSvetlana Bryant,Julie-Anne Balouek,Luke T Geiger,David J Barker,Catherine J PeñaPubMed 36412100
- Cellular and molecular gastroenterology and hepatology 15:13-372022Protein Tyrosine Kinase 7 (PTK7) Promotes Metastasis in Hepatocellular Carcinoma via SOX9 Regulation and TGF-β Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTsz Lam Matthew Wong,Tin-Lok Wong,Lei Zhou,Kwan Man,James Purcell,Terence K Lee,Jing-Ping Yun,Stephanie MaPubMed 36202326
- Cell chemical biology 29:1273-1287.e82022Temporal resolution of gene derepression and proteome changes upon PROTAC-mediated degradation of BCL11A protein in erythroid cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStuti Mehta,Altantsetseg Buyanbat,Yan Kai,Ozge Karayel,Seth Raphael Goldman,Davide Seruggia,Kevin Zhang,Yuko Fujiwara,Katherine A Donovan,Qian Zhu,Huan Yang,Behnam Nabet,Nathanael S Gray,Matthias Mann,Eric S Fischer,Karen Adelman,Stuart H OrkinPubMed 35839780
- Frontiers in cell and developmental biology 10:8374862022Transcriptional, Post-Transcriptional, and Post-Translational Mechanisms Rewrite the Tubulin Code During Cardiac Hypertrophy and Failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSai Aung Phyo,Keita Uchida,Christina Yingxian Chen,Matthew A Caporizzo,Kenneth Bedi,Joanna Griffin,Kenneth Margulies,Benjamin L ProsserPubMed 35433678
- eLife 10:2021Sumoylation of the human histone H4 tail inhibits p300-mediated transcription by RNA polymerase II in cellular extracts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCalvin Jon A Leonen,Miho Shimada,Caroline E Weller,Tomoyoshi Nakadai,Peter L Hsu,Elizabeth L Tyson,Arpit Mishra,Patrick Mm Shelton,Martin Sadilek,R David Hawkins,Ning Zheng,Robert G Roeder,Champak ChatterjeePubMed 34747692
- microPublication biology 2021:2021Changes in Histone H3 Acetylation on Lysine 9 Accompany Aβ 1-40 Overexpression in an Alzheimer's Disease Yeast Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuna M Hugais,Samantha N Cobos,Seth A Bennett,Jailene Paredes,Genevieve Foran,Mariana P TorrentePubMed 34746682
- Cancers 13:2021Functional Analysis of Non-Genetic Resistance to Platinum in Epithelial Ovarian Cancer Reveals a Role for the MBD3-NuRD Complex in Resistance Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTabea L Bauer,Katrin Collmar,Till Kaltofen,Ann-Katrin Loeffler,Lorena Decker,Jan Mueller,Sabine Pinter,Stephan A Eisler,Sven Mahner,Patricia Fraungruber,Stefan Kommoss,Annette Staebler,Lewis Francis,R Steven Conlan,Johannes Zuber,Udo Jeschke,Fabian Trillsch,Philipp RathertPubMed 34359703
Key facts
- Isotype
- IgG3
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg for 25 µg chromatin | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Recommended products
Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) is a mouse monoclonal antibody detecting Histone H3 in Western Blot, IP, ChIP. Suitable for Human, Mouse, Rat.
- Over 50 publications
- Trusted since 2005
Key facts
- Isotype
- IgG3
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- mAbcam 24834
- Purity
- IgG fraction
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), ChIP in Human, Mouse, Rat samples.
What is the molecular weight of Histone H3?
Anti-Histone H3 [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) is often used as a loading control in Western blot. It specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa.
Trusted by the scientific community
Anti-Histone H3 [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) was first used in a scientific publication in 2005 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-Histone H3 [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) has over 5 independent reviews from customers.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-mabcam-24834-nuclear-loading-control-and-chip-grade-ab24834--western-blot-img53029.jpg "Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834)")
Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834)
All lanes: Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) at 1 µg/mL
Lane 1: NIH/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 2: Testis (Mouse) Tissue Lysate at 10 µg
Lane 3: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 4: Testis (Rat) Tissue Lysate - normal tissue (ab29388) at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 18 kDa
Exposure time: 3min
![ChIP - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-mabcam-24834-nuclear-loading-control-and-chip-grade-ab24834--chip-img16667.jpg "ChIP - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834)")
ChIP - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834)
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 5μg of ab24834 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
![Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-antibody-mabcam-24834-nuclear-loading-control-and-chip-grade-ab24834--western-blot-img99045.jpg "Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834)")
Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834)
All lanes: Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) at 1 µg/mL
All lanes: HeLa histone prep at 1 µg/mL
SecondaryAll lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (Rabbit Anti-Mouse IgG H&L (HRP) ab6728) at 1/5000 dilution
Predicted band size: 15 kDa
Observed band size: 18 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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