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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit anti-Histone H3 antibody ab1791 is a rabbit polyclonal antibody that is used in Histone H3 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.
Tried and trusted by researchers since 2002
Anti-Histone H3 antibody ab1791 is cited in over 5010 publications
Same trusted quality, New lower price!
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
IHC-P | ChIP | ICC/IF | IP | WB | |
---|---|---|---|---|---|
Human | Expected | Tested | Tested | Expected | Tested |
Mouse | Expected | Expected | Expected | Expected | Tested |
Rat | Expected | Expected | Expected | Expected | Expected |
Arabidopsis thaliana | Expected | Expected | Expected | Expected | Expected |
Caenorhabditis elegans | Predicted | Predicted | Predicted | Predicted | Predicted |
Candida albicans | Predicted | Predicted | Predicted | Predicted | Predicted |
Chicken | Predicted | Predicted | Predicted | Predicted | Predicted |
Cyanidioschyzon merolae | Predicted | Predicted | Predicted | Predicted | Predicted |
Dictyostelium discoideum | Predicted | Predicted | Predicted | Predicted | Predicted |
Dog | Predicted | Predicted | Predicted | Predicted | Predicted |
Drosophila melanogaster | Expected | Expected | Expected | Expected | Tested |
Ferret | Predicted | Predicted | Predicted | Predicted | Predicted |
Indian muntjac | Expected | Expected | Expected | Expected | Expected |
Mammals | Predicted | Predicted | Predicted | Predicted | Predicted |
Neurospora crassa | Predicted | Predicted | Predicted | Predicted | Predicted |
Oncorhynchus mykiss | Predicted | Predicted | Predicted | Predicted | Predicted |
Rice | Predicted | Predicted | Predicted | Predicted | Predicted |
Saccharomyces cerevisiae | Expected | Expected | Expected | Expected | Tested |
Schistosoma mansoni | Predicted | Predicted | Predicted | Predicted | Predicted |
Schizosaccharomyces pombe | Expected | Expected | Expected | Expected | Tested |
Silk worm | Predicted | Predicted | Predicted | Predicted | Predicted |
Toxoplasma gondii | Predicted | Predicted | Predicted | Predicted | Predicted |
Xenopus laevis | Expected | Expected | Expected | Expected | Expected |
Zebrafish | Predicted | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Arabidopsis thaliana | Dilution info 1/100.00000 - 1/400.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Indian muntjac | Dilution info 1/100.00000 - 1/400.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/100.00000 - 1/400.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100.00000 - 1/400.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/400.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Saccharomyces cerevisiae | Dilution info 1/100 - 1/400 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Xenopus laevis | Dilution info 1/100 - 1/400 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Drosophila melanogaster | Dilution info 1/100 - 1/400 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Schizosaccharomyces pombe | Dilution info 1/100 - 1/400 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Dog, Caenorhabditis elegans, Ferret, Zebrafish, Mammals, Silk worm, Dictyostelium discoideum, Oncorhynchus mykiss, Neurospora crassa, Toxoplasma gondii, Rice, Schistosoma mansoni, Candida albicans, Cyanidioschyzon merolae | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2 µg for 106 Cells | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Schizosaccharomyces pombe | Dilution info - | Notes - |
Species Saccharomyces cerevisiae | Dilution info - | Notes - |
Species Drosophila melanogaster | Dilution info - | Notes - |
Species Indian muntjac | Dilution info 2 µg for 106 Cells | Notes - |
Species Arabidopsis thaliana | Dilution info 2 µg for 106 Cells | Notes - |
Species Xenopus laevis | Dilution info 2 µg for 106 Cells | Notes - |
Species Rat | Dilution info 2 µg for 106 Cells | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Dog, Caenorhabditis elegans, Ferret, Zebrafish, Mammals, Silk worm, Dictyostelium discoideum, Oncorhynchus mykiss, Neurospora crassa, Toxoplasma gondii, Rice, Schistosoma mansoni, Candida albicans, Cyanidioschyzon merolae | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Schizosaccharomyces pombe | Dilution info - | Notes - |
Species Saccharomyces cerevisiae | Dilution info - | Notes - |
Species Drosophila melanogaster | Dilution info - | Notes - |
Species Arabidopsis thaliana | Dilution info 1 µg/mL | Notes - |
Species Indian muntjac | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Xenopus laevis | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Dog, Caenorhabditis elegans, Ferret, Zebrafish, Mammals, Silk worm, Dictyostelium discoideum, Oncorhynchus mykiss, Neurospora crassa, Toxoplasma gondii, Rice, Schistosoma mansoni, Candida albicans, Cyanidioschyzon merolae | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Indian muntjac | Dilution info 5 µg/mL | Notes - |
Species Arabidopsis thaliana | Dilution info 5 µg/mL | Notes - |
Species Human | Dilution info 5 µg/mL | Notes - |
Species Mouse | Dilution info 5 µg/mL | Notes - |
Species Rat | Dilution info 5 µg/mL | Notes - |
Species Saccharomyces cerevisiae | Dilution info 5 µg/mL | Notes - |
Species Xenopus laevis | Dilution info 5 µg/mL | Notes - |
Species Drosophila melanogaster | Dilution info 5 µg/mL | Notes - |
Species Schizosaccharomyces pombe | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Dog, Caenorhabditis elegans, Ferret, Zebrafish, Mammals, Silk worm, Dictyostelium discoideum, Oncorhynchus mykiss, Neurospora crassa, Toxoplasma gondii, Rice, Schistosoma mansoni, Candida albicans, Cyanidioschyzon merolae | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. |
Species Schizosaccharomyces pombe | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. |
Species Saccharomyces cerevisiae | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. |
Species Drosophila melanogaster | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. |
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Indian muntjac | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. |
Species Arabidopsis thaliana | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. |
Species Xenopus laevis | Dilution info 1/1000 - 1/5000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Dog, Caenorhabditis elegans, Ferret, Zebrafish, Mammals, Silk worm, Dictyostelium discoideum, Oncorhynchus mykiss, Neurospora crassa, Toxoplasma gondii, Rice, Schistosoma mansoni, Candida albicans, Cyanidioschyzon merolae | Dilution info - | Notes - |
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FD, H3C7, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3FH, H3C8, HIST1H3F, H3FI, HIST1H3E, H3C6, HIST1H3D, H3FB, H3C4, HIST1H3C, H3FC, H3C3, HIST1H3B, H3FL, H3C2, HIST1H3A, H3FA, H3C1
Rabbit anti-Histone H3 antibody ab1791 is a rabbit polyclonal antibody that is used in Histone H3 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.
Tried and trusted by researchers since 2002
Anti-Histone H3 antibody ab1791 is cited in over 5010 publications
Same trusted quality, New lower price!
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FD, H3C7, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3FH, H3C8, HIST1H3F, H3FI, HIST1H3E, H3C6, HIST1H3D, H3FB, H3C4, HIST1H3C, H3FC, H3C3, HIST1H3B, H3FL, H3C2, HIST1H3A, H3FA, H3C1
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. Based only on sequence homology, we expect the antibody to react with multiple variants of H3 such as H3.1, H3.2 and H3.3.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
A recombinant rabbit monoclonal alternative is available to this target – ab176842
Rabbit polyclonal IgG (ab171870) is suitable for use as an isotype control with this antibody.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Histone H3 participates in chromatin remodeling and gene transcription regulation. As part of the nucleosome complex it influences DNA accessibility and subsequently gene expression. Through post-translational modifications including methylation and phosphorylation H3 modulates chromatin structure. Common modifications include mono- di- and tri-methylation at various lysine residues like K56 which affect transcriptional activation or repression. These modifications form a part of the histone code a framework for regulating chromatin dynamics and genetic information.
Histone H3 is a core component of the nucleosome playing an important role in DNA packaging within eukaryotic cells. Often referred to as H3 it has a molecular weight of around 15 kDa. Histone H3 is expressed in the nucleus of nearly all eukaryotic cells where it interacts with DNA and other histone proteins to form chromatin structure. Scientists frequently study histone H3 using techniques like Western blotting and immunohistochemistry utilizing antibodies such as anti-histone or phospho-histone H3 to distinguish post-translational modifications.
Histone H3 modifications are integral to several signaling pathways that modulate gene expression and cellular responses. The histone code interpreted by other proteins such as transcription factors and chromatin remodelers contributes significantly to the regulation of the cell cycle and signal transduction pathways like the MAPK/ERK pathway. H3 interacts closely with regulatory proteins including other histones and transcription machinery to facilitate these complex processes.
Histone H3 modifications are associated with various health conditions including cancer and neurological disorders. Aberrations in histone H3 methylation are linked to oncogenic transformations with disruptions often found in cancer types like glioblastoma and breast cancer. The alterations in H3 also connect to Alzheimer's disease through interactions with other histone modifications and their impact on gene expression patterns critical for neural function. Understanding these modifications helps provide insights into disease mechanisms and potential therapeutic targets.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Chromatin from Xenopus laevis oocytes was prepared according to the Abcam X-ChIP protocol.
Oocytes were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 mg of chromatin, 3 mg of ab7834 (anti-H3, light blue) and 3 μg of ab1791 (anti-H3, dark blue), and 20 ml of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow).
The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
The ChIP was performed with chromatin from mouse gut cell lysate and ab1791 at 1/250 dilution.
Negative control: No antibody was used (right bar).
The immunoprecipitated DNA was quantified by real time PCR.
Histone H3 - ChIP Grade was immunoprecipitated using 0.5mg HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract, 5 μg of Rabbit polyclonal to and 50 μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10 minutes, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.
Proteins were eluted by addition of 40 μl SDS loading buffer and incubated for 10 minutes at 70°C; 10 μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab1791.
Secondary Antibody: Mouse anti-rabbit HRP light chain (HRP) (ab99697).
Band: 15kDa; Histone H3 - ChIP Grade
All lanes: Immunoprecipitation - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Exposure time: 30s
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab1791 overnight at 4°C.
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody was used for detection.
Antibody binding was visualised using ECL development solution ab133406.
All lanes: Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791) at 1/1000 dilution
Lanes 1 and 4: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lanes 2 and 5: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg
Lanes 3 and 6: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 10s
ab1791 staining Histone H3 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab1791 at 0.1 μg/mL and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
ab1796 staining Histone H3 in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde, permeabilized with 0.05% Triton X-100 in PBS for 30 minutes and blocked with 5% BSA for 1 hour; antigen retrieval was by heat mediation in sodium citrate pH 6. Samples were incubated with the primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
Blocked with 3% milk for 1 hour at 25°C.
Incubated with the primary antibody for 16 hours at 4°C in 3% milk in TBS-tween.
All lanes: Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791) at 1/1000 dilution
Lane 1: Mouse skeletal muscle mitochondrial fraction at 20 µg
Lane 2: Mouse skeletal muscle nuclear fraction at 20 µg
All lanes: HRP-conjugated goat anti-rabbit IgG at 1/4000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 7min
ab1791 staining Histone H3 in HeLa (Human epithelial cell line from cervix adenocarcinoma) by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Samples were incubated with the primary antibody (1/300 in PBS + 0.2% gelatin) for 20 minutes at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Green - Histone H3.
Blue - DAPI.
Red - Tubulin.
ab1791 is tested in western blot on a range of species. We recommend loading higher amounts of protein (20-30ug) to increase the signal in yeast lysates
All lanes: Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - NIH/3T3 whole cell lysate (AB7179) at 10 µg
Lane 3: Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg
Lane 4: S.cerevisiae (Y190) Whole Cell Lysate at 10 µg
Lane 5: S.pombe Whole Cell Lysate at 10 µg
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Rabbit polyclonal to Histone H3 (ab1791) at 1/5000 on S. cerevisiae whole cell lysate (40 ug per lane).
Protein resolved on 15% SDS-PAGE gel. After transfer to PVDF membrane, blots were blocked in 1X PBS, 0.1% Tween-20, and 5% milk. ab1791 was diluted in 5 ml blocking buffer at 1/5000. Blots plus primary antibodies were either incubated overnight at 4°C or at RT for 2 hours. Blots were washed 6X for 10 minutes each in PBS with 0.1% Tween-20 before addition of secondary antibodies. Secondary antibodies were diluted 1/2,000 in blocking buffer and incubated with blots for 2 hours at RT. Secondary blots were washed 4X for 10 minutes each in PBS with 0.1% Tween-20 and 2X for 10 minutes each in PBS.
All lanes: Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)
Predicted band size: 15 kDa
Paraffin-embedded rat brain tissue stained for Histone H3 using ab1791 at 1/8000 dilution in immunohistochemical analysis.
ab1791 staining Histone H3 in human infantile fibromatosis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for 3 hours at room temperature; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/100 in TBS + 1% BSA + 1% FBS) for 16 hours. An un-diluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
ab1791 staining Histone H3 (red) in rat brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde, permeabilized with 0.1% TBS-TritonX and blocked with 10% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with the primary antibody (1/500 in 10% normal goat serum) for 24 hours at 24°C. An Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Green - Nucleus staining.
Red - Histone H3 staining.
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab1791 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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