AB194706

Anti-Histone H3 (asymmetric di methyl R2) antibody

Name: Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706) | Abcam
Brand: Abcam Limited
SKU: AB194706
Price: 545 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal H3 asymmetric di methyl R2 antibody. Suitable for Dot, WB, IHC-P, ICC/IF and reacts with Synthetic peptide, Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human H3-4 asymmetric di methyl R2.

View Alternative Names

H3FT, HIST3H3, H3-4, Histone H3.1t, H3/t, H3t, H3/g, Histone H3.4, H3R2me2, H3R2me, H3R2, H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3R2me2, H3R2me, H3R2

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Paraffin-embedded human colon tissue stained for Histone H3 (asymmetric di methyl R2) using ab194706 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Immunofluorescence staining of 293T cells stained for Histone H3 (asymmetric di methyl R2) with ab194706. Nuclei are labeled with DAPI (Blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Immunofluorescence staining of HeLa cells stained for Histone H3 (asymmetric di methyl R2) with ab194706 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Paraffin-embedded mouse brain tissue stained for Histone H3 (asymmetric di methyl R2) using ab194706 at 1/100 dilution in immunohistochemical analysis.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Immunofluorescence staining of NIH/3T3 stained for Histone H3 (asymmetric di methyl R2) with ab194706 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Paraffin-embedded rat brain tissue stained for Histone H3 (asymmetric di methyl R2) using ab194706 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

H3 protein at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 15 kDa

true

Exposure time: 10s

Supplier Data

Dot Blot - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Dot-blot analysis of methylation peptides using ab194706.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Dot, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human H3-4 asymmetric di methyl R2. The exact immunogen used to generate this antibody is proprietary information.

Q16695

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Rat": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.05% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3-4 asymmetric di methyl R2

Additional targets

H3C1 asymmetric di methyl R2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 64: PubMed38299254

2024

Inhibition of protein arginine methyltransferase 6 activates interferon signaling and induces the apoptosis of endometrial cancer cells via histone modification.

Applications

Unspecified application

Species

Unspecified reactive species

Futaba Inoue,Kenbun Sone,Kohei Kumegawa,Ryuta Hachijo,Eri Suzuki,Saki Tanimoto,Natsumi Tsuboyama,Kosuke Kato,Yusuke Toyohara,Yu Takahashi,Misako Kusakabe,Asako Kukita,Harunori Honjoh,Akira Nishijima,Ayumi Taguchi,Yuichiro Miyamoto,Michihiro Tanikawa,Takayuki Iriyama,Mayuyo Mori,Osamu Wada-Hiraike,Katsutoshi Oda,Hiromu Suzuki,Reo Maruyama,Yutaka Osuga

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2202737 PubMed36941223

2023

The PRMT6/PARP1/CRL4B Complex Regulates the Circadian Clock and Promotes Breast Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tianshu Yang,Wei Huang,Tianyu Ma,Xin Yin,Jingyao Zhang,Miaomiao Huo,Ting Hu,Tianyang Gao,Wei Liu,Die Zhang,Hefen Yu,Xu Teng,Min Zhang,Hao Qin,Yunkai Yang,Baowen Yuan,Yan Wang

Nature communications 14:1430 PubMed36932056

2023

A mycobacterial effector promotes ferroptosis-dependent pathogenicity and dissemination.

Applications

Unspecified application

Species

Unspecified reactive species

Lihua Qiang,Yong Zhang,Zehui Lei,Zhe Lu,Shasha Tan,Pupu Ge,Qiyao Chai,Mengyuan Zhao,Xinwen Zhang,Bingxi Li,Yu Pang,Lingqiang Zhang,Cui Hua Liu,Jing Wang

Cell reports 30:1208-1222.e9 PubMed31995759

2020

PRMT1 Is Recruited via DNA-PK to Chromatin Where It Sustains the Senescence-Associated Secretory Phenotype in Response to Cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele Musiani,Roberto Giambruno,Enrico Massignani,Marica Rosaria Ippolito,Marianna Maniaci,Sriganesh Jammula,Daria Manganaro,Alessandro Cuomo,Luciano Nicosia,Diego Pasini,Tiziana Bonaldi

Experimental cell research 373:155-163 PubMed30342005

2018

YAP via interacting with STAT3 regulates VEGF-induced angiogenesis in human retinal microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Manhui Zhu,Xiaojuan Liu,Ying Wang,Lili Chen,Li Wang,Xiao Qin,Jiaowen Xu,Lele Li,Yuanyuan Tu,Taohu Zhou,Aimin Sang,E Song

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (asymmetric di methyl R2) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Western blot - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Dot Blot - Anti-Histone H3 (asymmetric di methyl R2) antibody (AB194706)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (5)

Product promise