AB176845

Anti-Histone H3 (asymmetric di methyl R2) antibody [EPR17705]

RabMAb
Advanced Validation
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal H3 asymmetric di methyl R2 antibody. Suitable for WB, PepArr and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3R2me2, H3R2me, H3R2

2 Images

Supplier Data

Western blot - Anti-Histone H3 (asymmetric di methyl R2) antibody [EPR17705] (AB176845)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Histone H3 (asymmetric di methyl R2) antibody [EPR17705] (ab176845) at 1/200 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

Lane 2:

NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 15s

Peptide Array - Anti-Histone H3 (asymmetric di methyl R2) antibody [EPR17705] (AB176845)

PepArr

Supplier Data

Peptide Array - Anti-Histone H3 (asymmetric di methyl R2) antibody [EPR17705] (AB176845)

ab176845 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17705

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

PepArr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "", "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 asymmetric di methyl R2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:1303 PubMed35288557

2022

The arginine methyltransferase PRMT7 promotes extravasation of monocytes resulting in tissue injury in COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Gizem Günes Günsel,Thomas M Conlon,Aicha Jeridi,Rinho Kim,Zeynep Ertüz,Niklas J Lang,Meshal Ansari,Mariia Novikova,Dongsheng Jiang,Maximilian Strunz,Mariia Gaianova,Christine Hollauer,Christina Gabriel,Ilias Angelidis,Sebastian Doll,Jeanine C Pestoni,Stephanie L Edelmann,Marlene Sophia Kohlhepp,Adrien Guillot,Kevin Bassler,Hannelore P Van Eeckhoutte,Özgecan Kayalar,Nur Konyalilar,Tamara Kanashova,Sophie Rodius,Carolina Ballester-López,Carlos M Genes Robles,Natalia Smirnova,Markus Rehberg,Charu Agarwal,Ioanna Krikki,Benoit Piavaux,Stijn E Verleden,Bart Vanaudenaerde,Melanie Königshoff,Gunnar Dittmar,Ken R Bracke,Joachim L Schultze,Henrik Watz,Oliver Eickelberg,Tobias Stoeger,Gerald Burgstaller,Frank Tacke,Vigo Heissmeyer,Yuval Rinkevich,Hasan Bayram,Herbert B Schiller,Marcus Conrad,Robert Schneider,Ali Önder Yildirim

View all publications

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peptideArrayWebsite

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (asymmetric di methyl R2) antibody [EPR17705]

Western blot - Anti-Histone H3 (asymmetric di methyl R2) antibody [EPR17705] (AB176845)

Peptide Array - Anti-Histone H3 (asymmetric di methyl R2) antibody [EPR17705] (AB176845)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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