AB194679

Anti-Histone H3 (asymmetric di methyl R26) antibody

Name: Anti-Histone H3 (asymmetric di methyl R26) antibody (ab194679) | Abcam
Brand: Abcam Limited
SKU: AB194679
Price: 535 USD
Availability: InStock
Rating: 2 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal H3 asymmetric di methyl R26 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human H3-4 asymmetric di methyl R26.

View Alternative Names

H3FT, HIST3H3, H3-4, Histone H3.1t, H3/t, H3t, H3/g, Histone H3.4, H3R26me2, H3R26me, H3R26, H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3R26me2, H3R26me, H3R26

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R26) antibody (AB194679)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R26) antibody (AB194679)

Immunofluorescent analysis of 293T cells labeling Histone H3 (asymmetric di methyl R26) with ab194679. Nuclei are labeled with DAPI (Blue).

Supplier Data

Western blot - Anti-Histone H3 (asymmetric di methyl R26) antibody (AB194679)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-Histone H3 (asymmetric di methyl R26) antibody (ab194679)

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

Histone H3 protein expressed in E.coli at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human H3-4 asymmetric di methyl R26. The exact immunogen used to generate this antibody is proprietary information.

Q16695

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3-4 asymmetric di methyl R26

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:670 PubMed39266534

2024

The NRF2-CARM1 axis links glucose sensing to transcriptional and epigenetic regulation of the pentose phosphate pathway in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Ping,Guangyao Li,Qijiao Li,Yang Fang,Taotao Fan,Jing Wu,Ruiyi Zhang,Lesha Zhang,Bing Shen,Jizheng Guo

Protein & cell 15:744-765 PubMed38476024

2024

CARM1 drives triple-negative breast cancer progression by coordinating with HIF1A.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Feng,Jie Gao,Ruiqiong Liu,Wei Liu,Tianyang Gao,Yunkai Yang,Die Zhang,Tianshu Yang,Xin Yin,Hefen Yu,Wei Huang,Yan Wang

Molecular therapy. Nucleic acids 34:102063 PubMed38028203

2023

A CRISPR-Cas9 library screening identifies CARM1 as a critical inhibitor of ferroptosis in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yiming Cheng,Xiaochen Wang,Shuyu Huang,Liang Zhang,Bei Lan,Xuanyuan Li,Hao Chen,Zhenfeng Liu,Yijie Su,Lishan Xi,Shengyun Feng,Yanxuan Guo,Jun Zhou,Yingmei Wang,Chenghao Xuan

Aging 12:10578-10593 PubMed32487779

2020

CARM1 promotes non-small cell lung cancer progression through upregulating CCNE2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Deqin Wu,Jing He,Wei Zhang,Kai Wang,Shidai Jin,Jun Li,Wen Gao

Nature 527:249-53 PubMed26503055

2015

Epigenetic silencing of TH1-type chemokines shapes tumour immunity and immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Dongjun Peng,Ilona Kryczek,Nisha Nagarsheth,Lili Zhao,Shuang Wei,Weimin Wang,Yuqing Sun,Ende Zhao,Linda Vatan,Wojciech Szeliga,Jan Kotarski,Rafał Tarkowski,Yali Dou,Kathleen Cho,Sharon Hensley-Alford,Adnan Munkarah,Rebecca Liu,Weiping Zou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (asymmetric di methyl R26) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R26) antibody (AB194679)

Western blot - Anti-Histone H3 (asymmetric di methyl R26) antibody (AB194679)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise