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Rabbit Recombinant Monoclonal H3 citrulline R2 antibody. Suitable for IP, I-ELISA, WB, PepArr and reacts with Transfected cell lysate, Mouse, Human, Recombinant fragment - Human samples. Cited in 13 publications.


Images

Immunoprecipitation - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (AB176843), expandable thumbnail
  • Indirect ELISA - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (AB176843), expandable thumbnail
  • Western blot - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (AB176843), expandable thumbnail
  • Peptide Array - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (AB176843), expandable thumbnail
  • Western blot - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (AB176843), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPI-ELISAWBPepArr
Human
Expected
Expected
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Recombinant fragment - Human
Not recommended
Tested
Not recommended
Not recommended
Transfected cell lysate
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Transfected cell lysate

Dilution info

1/30

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

0.1 µg/mL

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Species

Human

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate, Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate, Recombinant fragment - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal H3 citrulline R2 antibody. Suitable for IP, I-ELISA, WB, PepArr and reacts with Transfected cell lysate, Mouse, Human, Recombinant fragment - Human samples. Cited in 13 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR17703

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843), expandable thumbnail

    Immunoprecipitation - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843)

    Histone H3 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 ?M lonomycin for 4 hours, whole cell lysate 10 ug with ab176843 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab176843 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 ?M lonomycin for 4 hours, whole cell lysate 10 ug

    Lane 2: ab176843 IP in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 ?M lonomycin for 4 hours whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab176843 in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 ?M lonomycin for 4 hours whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min

    All lanes: Immunoprecipitation - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843)

    Predicted band size: 15 kDa

  • Indirect ELISA - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843), expandable thumbnail

    Indirect ELISA - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843)

    ELISA analysis of Human Histone H3 (ci recombinant protein at 100 ng/ml with ab176843. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • Western blot - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843), expandable thumbnail

    Western blot - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    PADI4 catalyzes the citrullination of arginine residues of histone proteins.

    All lanes: Western blot - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843) at 1/500 dilution

    Lane 1: MCF7 (Human breast adenocarcinoma cell line) cells transfected with vector control

    Lane 2: MCF7 (Human breast adenocarcinoma cell line) cells transfected with PADI4

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

    Exposure time: 30s

  • Peptide Array - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843), expandable thumbnail

    Peptide Array - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843)

    ab176843 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.

  • Western blot - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843), expandable thumbnail

    Western blot - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    PADI4 catalyzes the citrullination of arginine residues of histone proteins.

    All lanes: Western blot - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843) at 1/500 dilution

    Lane 1: NIH/3T3 (Mouse embyro fibroblast cells) cells transfected with vector control

    Lane 2: NIH/3T3 (Mouse embyro fibroblast cells) cells transfected with PADI4

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

    Exposure time: 30s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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