Anti-Histone H3 (citrulline R2) antibody [EPR17703] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-Histone H3 (citrulline R2) antibody [EPR17703] - BSA and Azide free (AB203954)

This data was developed using ab176843, the same antibody clone in a different buffer formulation.Histone H3 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 ?M lonomycin for 4 hours, whole cell lysate 10 ug with ab176843 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab176843 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 ?M lonomycin for 4 hours, whole cell lysate 10 ug

Lane 2 : ab176843 IP in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 ?M lonomycin for 4 hours whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab176843 in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 ?M lonomycin for 4 hours whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 min

All lanes:

Immunoprecipitation - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (<a href='/en-us/products/primary-antibodies/histone-h3-citrulline-r2-antibody-epr17703-ab176843'>ab176843</a>)

Predicted band size: 15 kDa

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• I-ELISA

Supplier Data

Indirect ELISA - Anti-Histone H3 (citrulline R2) antibody [EPR17703] - BSA and Azide free (AB203954)

This data was developed using ab176843, the same antibody clone in a different buffer formulation.ELISA analysis of Human Histone H3 (ci recombinant protein at 100 ng/ml with ab176843. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

• PepArr

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Peptide Array - Anti-Histone H3 (citrulline R2) antibody [EPR17703] - BSA and Azide free (AB203954)

ab176843 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176843).