Rabbit Polyclonal H3 citrulline R2 antibody. Suitable for IP, WB, PepArr and reacts with Recombinant fragment - Human, Human, Mouse, Rat, Synthetic peptide samples. Cited in 596 publications.
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- Science advances 9:eadj13972023NET formation is a default epigenetic program controlled by PAD4 in apoptotic neutrophils.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanfang Peipei Zhu,Mary Speir,ZheHao Tan,Jamie Casey Lee,Cameron J Nowell,Alyce A Chen,Hajera Amatullah,Ari J Salinger,Carolyn J Huang,Gio Wu,Weiqi Peng,Kasra Askari,Eric Griffis,Majid Ghassemian,Jennifer Santini,Motti Gerlic,William B Kiosses,Sergio D Catz,Hal M Hoffman,Kimberly F Greco,Edie Weller,Paul R Thompson,Lai Ping Wong,Ruslan Sadreyev,Kate L Jeffrey,Ben A CrokerPubMed 38117877
- Journal of inflammation research 16:5629-56462023Neutrophil Extracellular Traps Aggravate Contrast-Induced Acute Kidney Injury by Damaging Glomeruli and Peritubular Capillaries.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeng Wang,Tingting Gao,Ruijing Zhang,Jie Hu,Siqi Gao,Yuwen Wang,Xiaotong Qi,Yun Zhou,Guoping Zheng,Honglin DongPubMed 38046404
- Frontiers in immunology 14:12236532023The Rac-GEF Tiam1 controls integrin-dependent neutrophil responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKirsti Hornigold,Martin J Baker,Polly A Machin,Stephen A Chetwynd,Anna-Karin Johnsson,Chiara Pantarelli,Priota Islam,Melanie Stammers,Laraine Crossland,David Oxley,Hanneke Okkenhaug,Simon Walker,Rachael Walker,Anne Segonds-Pichon,Yoshinori Fukui,Angeliki Malliri,Heidi C E WelchPubMed 38077328
- Precision clinical medicine 6:pbad0252023Blockade of Syk modulates neutrophil immune-responses via the mTOR/RUBCNL-dependent autophagy pathway to alleviate intestinal inflammation in ulcerative colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengqin Zhu,Dehuai Jing,Huihui Zhou,Zongjing Hu,Yan Wang,Guiyuan Jin,Yonghong Yang,Guangxi ZhouPubMed 37941642
- Science advances 9:eadj10192023Inhibition of neutrophil extracellular trap formation alleviates vascular dysfunction in type 1 diabetic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Liu,Srilakshmi Yalavarthi,Ajay Tambralli,Lixia Zeng,Christine E Rysenga,Nikoo Alizadeh,Lucas Hudgins,Wenying Liang,Somanathapura K NaveenKumar,Hui Shi,Miriam A Shelef,Kevin B Atkins,Subramaniam Pennathur,Jason S KnightPubMed 37878711
- Scientific reports 13:179922023Protein citrullination and NET formation do not contribute to the pathology of A20/TNFAIP3 mutant mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarel F A Van Damme,Pieter Hertens,Arne Martens,Elisabeth Gilis,Dario Priem,Inge Bruggeman,Amelie Fossoul,Jozefien Declercq,Helena Aegerter,Andy Wullaert,Tino Hochepied,Esther Hoste,Lieselotte Vande Walle,Mohamed Lamkanfi,Savvas N Savvides,Dirk Elewaut,Bart N Lambrecht,Geert van LooPubMed 37865713
- FEMS microbes 4:xtad0192023suppresses -induced NETosis and promotes bacterial survival in polymicrobial infections.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatrick Hsien-Neng Kao,Jun-Hong Ch'ng,Kelvin K L Chong,Claudia J Stocks,Siu Ling Wong,Kimberly A KlinePubMed 37900578
- Pulmonary circulation 13:e122952023Lianhua Qingke ameliorates lipopolysaccharide-induced lung injury by inhibiting neutrophil extracellular traps formation and pyroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjun Peng,Hui Qi,Wensi Zhu,Lin Tong,Ainiwaer Rouzi,Yuanyuan Wu,Linxiao Han,Ludan He,Yu Yan,Ting Pan,Jie Liu,Qin Wang,Zhenhua Jia,Yuanlin Song,Qiaoliang Zhu,Jian ZhouPubMed 37808899
- Nature communications 14:61792023Gut microbiota aggravates neutrophil extracellular traps-induced pancreatic injury in hypertriglyceridemic pancreatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuanqun Li,Liwei Liu,Tianqi Lu,Yuhang Sui,Can Zhang,Yongwei Wang,Tao Zhang,Yu Xie,Peng Xiao,Zhongjie Zhao,Chundong Cheng,Jisheng Hu,Hongze Chen,Dongbo Xue,Hua Chen,Gang Wang,Rui Kong,Hongtao Tan,Xuewei Bai,Zhibo Li,Florencia McAllister,Le Li,Bei SunPubMed 37794047
- Frontiers in immunology 14:12590042023Nuclease activity and protein A release of clinical isolates determine the virulence in a murine model of acute lung infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNadine Ludwig,Julia Thörner-van Almsick,Sina Mersmann,Bernadette Bardel,Silke Niemann,Achmet Imam Chasan,Michael Schäfers,Andreas Margraf,Jan Rossaint,Barbara C Kahl,Alexander Zarbock,Helena BlockPubMed 37849760
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IP | WB | PepArr | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Not recommended |
| Mouse | Expected | Tested | Expected | Not recommended |
| Rat | Expected | Tested | Expected | Not recommended |
| Cow | Predicted | Predicted | Predicted | Not recommended |
| Monkey | Predicted | Predicted | Predicted | Not recommended |
| Rabbit | Predicted | Predicted | Predicted | Not recommended |
| Recombinant fragment - Human | Expected | Not recommended | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
Species Human | Dilution info - | Notes - |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes In our hands, when tested in western blot, this product typically gives a weaker signal in mouse and rat tissue lysates compared to mouse and rat cell lines. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. Abcam recommends using 3-5% milk as the blocking agent We recommend Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody. |
Species Rat | Dilution info 1 µg/mL | Notes In our hands, when tested in western blot, this product typically gives a weaker signal in mouse and rat tissue lysates compared to mouse and rat cell lines. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. Abcam recommends using 3-5% milk as the blocking agent We recommend Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody. |
Species Human | Dilution info 1 µg/mL | Notes In our hands, when tested in western blot, this product typically gives a weaker signal in mouse and rat tissue lysates compared to mouse and rat cell lines. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. Abcam recommends using 3-5% milk as the blocking agent We recommend Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Synthetic peptide | Dilution info - | Notes - |
PepArr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Rabbit, Cow, Monkey, Recombinant fragment - Human, Synthetic peptide | Dilution info - | Notes - |
Associated Products
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Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C3, H3FA, H3FB, H3FC, HIST1H3A, H3C2, HIST1H3B, H3C8, H3C1, HIST1H3H, H3C11, H3FL, HIST1H3F, HIST1H3E, H3FH, H3C7, H3FI, H3C4, HIST1H3C, H3FJ, HIST1H3G, H3C12, HIST1H3J, H3C10, HIST1H3I, H3FF, H3FK, HIST1H3D, H3C6, H3FD
Recommended products
Rabbit Polyclonal H3 citrulline R2 antibody. Suitable for IP, WB, PepArr and reacts with Recombinant fragment - Human, Human, Mouse, Rat, Synthetic peptide samples. Cited in 596 publications.
Alternative names
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C3, H3FA, H3FB, H3FC, HIST1H3A, H3C2, HIST1H3B, H3C8, H3C1, HIST1H3H, H3C11, H3FL, HIST1H3F, HIST1H3E, H3FH, H3C7, H3FI, H3C4, HIST1H3C, H3FJ, HIST1H3G, H3C12, HIST1H3J, H3C10, HIST1H3I, H3FF, H3FK, HIST1H3D, H3C6, H3FD
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
ab5103 detects a 17 kDa band in single lane Western Blot. Peptide inhibition in Western Blot hasn't been processed. Modification specificity is determined by Peptide Array. ab5103 binds strongly to Histone H3 citrulline 2 + 8 + 17 peptide.
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab281584.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Immunoprecipitation - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
This immunoprecipitation image is a comparison between ab5103 and the alternative recombinant multiclonal antibody Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] ab281584.
Left side - Recombinant multiclonal to Histone H3 (citrulline R2 + R8 + R17) - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] ab281584
All lanes: Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] ab281584 at 1/1000 dilution.
Lane 1: HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysate 10 μg.
Lane 2: Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] ab281584 IP in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] ab281584 in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours whole cell lysateRight side:Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
All lanes: Anti-Histone H3 (citrulline R2 + R8 + R17) antibody ab5103 at 1/1000 dilution.
Lane 1: HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysate 10 μg.
Lane 2: ab5103 IP in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab5103 in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours whole cell lysateSecondary
All lanes: VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Predicted band size: 15kDaWhy choose a recombinant antibody?
Research with confidence – consistent and reproducible results with every batch
Long-term and scalable supply – powered by recombinant technology for fast production
Success from the first experiment – confirmed specificity through extensive validation
Ethical standards compliant – production is animal-freeAll lanes: Immunoprecipitation - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
Predicted band size: 15 kDa
Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
Blocking buffer: 2% BSA
Gel type: MES
All lanes: Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) at 1 µg/mL
Lane 1: Mouse brain tissue lysate at 10 µg
Lane 2: Rat brain tissue lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 1min
Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
This western blot image is a comparison between ab5103 and the alternative recombinant multiclonal antibody Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] ab281584.
Left side - Recombinant multiclonal to Histone H3 (citrulline R2 + R8 + R17) - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] ab281584
All lanes: Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] ab281584 at 1/1000 dilution.
Lane 1: Untreated HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a myc-His-tag®, whole cell lysate
Lane 2: HEK-293T transfected with PADI4 expression vector containing a myc-His-tag® treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysate
Lane 3: Untreated NIH/3T3 (mouse embryonic fibroblast) transfected with PADI4 expression vector containing a myc-His-tag®, whole cell lysate
Lane 4: NIH/3T3 transfected with PADI4 expression vector containing a myc-His-tag® treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysateRight side: Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
All lanes: Anti-Histone H3 (citrulline R2 + R8 + R17) antibody ab5103 at 1/1000 dilution.
Lane 1: Untreated HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a myc-His-tag®, whole cell lysate
Lane 2: HEK-293T transfected with PADI4 expression vector containing a myc-His-tag® treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysate
Lane 3: Untreated NIH/3T3 (mouse embryonic fibroblast) transfected with PADI4 expression vector containing a myc-His-tag®, whole cell lysate
Lane 4: NIH/3T3 transfected with PADI4 expression vector containing a myc-His-tag® treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysateLysates/proteins at 20 μg per lane.
Secondary
All lanes: Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)Why choose a recombinant antibody?
Research with confidence – consistent and reproducible results with every batch
Long-term and scalable supply – powered by recombinant technology for fast production
Success from the first experiment – confirmed specificity through extensive validation
Ethical standards compliant – production is animal-freeAll lanes: Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
Predicted band size: 15 kDa
Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
Blocking buffer: 2% BSA
Gel type: MES
All lanes: Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) at 1 µg/mL
Lane 1: NIH/3T3 (Mouse embryo fibroblast cell line) nuclear lysate at 10 µg
Lane 2: PC12 (Rat adrenal pheochromocytoma cell line) nuclear lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 1min
Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
Loading Control: GAPDH
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab5103 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 secondary at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) at 0.2 µg/mL
Lane 1: HL60 whole cell lysate (negative control) at 20 µg
Lane 2: HL60 whole cell lysate + DMSO (solvent control) at 20 µg
Lane 3: HL60 whole cell lysate + DMSO + Calcium Ionophore (positive control) at 20 µg
SecondaryAll lanes: Goat anti Rabbit IR680 at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Peptide Array - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
All batches of ab5103 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - citrulline 2 + 8 + 17 peptide (Human Histone H3 (citrulline R2 + R8 + R17) peptide ab32876), indicating that this antibody specifically recognises the Histone H3 - citrulline 2 + 8 + 17 modifications.
Human Histone H3 (citrulline R2 + R8 + R17) peptide ab32876 - Histone H3 - citrulline 2 + 8 + 17
ab17566 - Histone H3 - unmodified
Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above .
Blots were developled with Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody
All lanes: Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) at 1 µg/mL
All lanes: HL60 (Human Caucasian promyelocytic leukaemia) DMSO and Calcium Ionophore treated Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 30s
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