Rabbit Recombinant Monoclonal H3 citrulline R26 antibody. Carrier free. Suitable for ELISA, Dot, WB and reacts with Synthetic peptide, Mouse, Human samples.
pH: 7.2 - 7.4
Constituents: PBS
ELISA | Dot | WB | |
---|---|---|---|
Human | Predicted | Predicted | Expected |
Mouse | Predicted | Predicted | Expected |
Synthetic peptide | Tested | Tested | Not recommended |
Species | Dilution info | Notes |
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Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Synthetic peptide | Dilution info - | Notes - |
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Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3R26cit
Rabbit Recombinant Monoclonal H3 citrulline R26 antibody. Carrier free. Suitable for ELISA, Dot, WB and reacts with Synthetic peptide, Mouse, Human samples.
pH: 7.2 - 7.4
Constituents: PBS
ab242414 is the carrier-free version of Anti-Histone H3 (citrulline R26) antibody [EPR20606] ab212082.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Dot blot analysis of Histone H3 (citrulline R26) labeled with Anti-Histone H3 (citrulline R26) antibody [EPR20606] ab212082 at 1/1000 dilution.
Lane 1: Histone H3 (citrulline R26) peptide (aa25-36)
Lane 2: Histone H3 (Citrulline R17) peptide (aa1-21)
Lane 3: Histone H3 (Citrulline R8) peptide (aa1-21)
Lane 4: Histone H3 (Citrulline R2) peptide (aa1-21)
Lane 5: Histone H3 peptide (aa1-21)
Lane 6: Histone H3 peptide (aa19-36)
Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3 (citrulline R26) antibody [EPR20606] ab212082)
ELISA using Anti-Histone H3 (citrulline R26) antibody [EPR20606] ab212082 labeling Histone H3 (Citrulline R26), Histone H3R26 unmodified, Histone H3 (Citrulline R17), Histone H3 (Citrulline R8), Histone H3 (Citrulline R2) and Histone H3R2/8/17 unmodified at 1 µg/ml. Anti-Histone H3 (citrulline R26) antibody [EPR20606] ab212082 was used at a range between 0 -1 µg/ml.
Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) was used as secondary antibody at 1/2500 dilution.
Anti-Histone H3 (citrulline R26) antibody [EPR20606] ab212082 cross binds H3R8Cit at 14%.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3 (citrulline R26) antibody [EPR20606] ab212082)
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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