AB219406

Anti-Histone H3 (citrulline R8) antibody [EPR20358-13]

RabMAb
Recombinant
20ul selling size
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(10 Publications)

Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (ab219406) is a rabbit monoclonal antibody detecting Histone H3 in Western Blot, Flow Cytometry (Intra), IP, ICC/IF, ELISA. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3R8cit

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells transfected with GFP only or a GFP-tagged PADI4 expression construct, then treated with 10mM CaCl₂ for 2 hours, labeling Histone H3 (citrulline R8) with ab219406 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing positive staining on HEK-293T cells transfected with a GFP-tagged PADI4 expression construct, then treated with 10mM CaCl₂ for 2 hours.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) transfected with empty vector (left panel) or PADI4 (WT, right panel), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, labeling Histone H3 (citrulline R8)with ab219406 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor^® 647) at 1/2000 dilution.

Positive signal is obtained from HEK-293T cells transfected with WT PADI4 treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours.

ELISA - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

ELISA

Supplier Data

ELISA - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

ELISA analysis of Human Histone H3 (citrulline R8) recombinant protein at 100 ng/ml with ab219406. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Lab

Immunoprecipitation - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Histone H3 (citrulline R8) was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) transfected with PADI4 (WT), then treated with 10mM CaCl₂ and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate with ab219406 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab219406 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl₂ and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate 10 μg (Input).
Lane 2 : ab219406 IP in HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl₂ and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219406 in HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl₂ and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (ab219406)

Predicted band size: 15 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (ab219406) at 1/1000 dilution

Lane 1:

E12 rat embryo lysate at 10 µg

Lane 2:

E12 mouse embryo lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa,43 kDa

Observed band size: 15 kDa

false

Exposure time: 1s

ELISA

Unknown

ELISA - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Direct ELISA using ab219406 at 0-1000 ng/ml, followed by Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution. Antigen concentration : 1000 ng/ml.

Lab

Western blot - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Blocking/Dilution buffer : 5% BSA/TBST.

Histone H3R8 is citrullinated by PADI4 and CaCl₂ is used as a cofactor according to the literature (PMID : 16567635). Ionomycin is used to improve the modification by PADI4 according to the literature (PMID : 26360112).

All lanes:

Western blot - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (ab219406) at 1/10000 dilution

Lane 1:

Whole cell lysate from HEK-293T (Human epithelial cell line from embryonic kidney) transfected with empty vector with GFP tag (vector control), then treated with 10mM CaCl2 and 10μM Ionomyci at 10 µg

Lane 2:

Whole cell lysate from HEK-293T cells transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (<a href='/en-us/products/biochemicals/ionomycin-ca2-salt-ca2-ionophore-ab120116'>ab120116</a>) for 2 hours at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 1s

Lab

Western blot - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (ab219406) at 1/5000 dilution

Lane 1:

Whole cell lysate from NIH/3T3 (Mouse embryonic fibroblast cell line) transfected with empty vector with GFP tag (vector control), then treated with 10mM CaCl2 for 2 hours at 10 µg

Lane 2:

Whole cell lysate from NIH/3T3 transfected with PADI4 (WT) then treated with 10mM CaCl2 for 2 hours at 10 µg

Lane 3:

Whole cell lysate from C6 (Rat glial tumor cell line) transfected with empty vector with GFP tag (vector control) then treated with 10mM CaCl2 and 10μM Ionomycin (<a href='/en-us/products/biochemicals/ionomycin-ca2-salt-ca2-ionophore-ab120116'>ab120116</a>) for 2 hours at 10 µg

Lane 4:

C6 transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (<a href='/en-us/products/biochemicals/ionomycin-ca2-salt-ca2-ionophore-ab120116'>ab120116</a>) for 2 hours at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 1s

Carrier free

Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Histone H3 (citrulline R8) antibody [EPR20358-13]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20358-13

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IP, ELISA, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (ab219406) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Mouse, Rat samples.

What is the molecular weight of Histone H3?
Anti-Histone H3 (citrulline R8) [EPR20358-13] (ab219406) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa.

Other related products
We have a range of other formats of antibody clone [EPR20358-13] also available for your convenience: ab219406, Carrier free - ab232939, Alexa Fluor^® 647 - ab300306

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

Treatment Conditions

Chemical treatment is required for detection of endogenous citrullinated proteins in cell lines. PADs (including PADI2 and PADI4) are the enzymes that hydrolyze arginine to citrulline, in the presence of Ca²⁺ and ionomycin. Please refer to PMID: 26360112

Controls:

- Mock (no transfection)
- Transfection reagent–only control
- Empty vector control
- Vehicle control for chemical treatment

Generation of PADI2 or PADI4 overexpression cell line and treatment with CaCl₂/Ionomycin

1. Prior to transfection with PADI2 or PADI4 expression vector, seed cells in complete medium and place in a 37ºC, 5% CO₂ incubator for 24 hours or until cells reach 70-80% confluence.
For a single T-175 flask we recommend seeding the following common cell lines as follows in 30 mL media:

- HEK-293T: 1x10⁷ cells
- MCF7: 2.5-4x10⁶ cells
- NIH3T3: 4-5x10⁶ cells
- C6: 3-4x10⁶ cells

2. Once cells have reached confluence, prepare the PADI2 or PADI4 expression vector for transfection using your preferred method (PMID: 33976969).
3. 24-48 hours after transfection, remove the medium from the cells and replace with fresh complete medium containing 10 mM CaCl₂ (ab285384) and 10 uM Ionomycin (ab120116) and incubate for 2-4 hours.

Key Considerations:

- For alternative cell lines, you should confirm the transfectability, growth rate, and sensitivity of the cell line to the planned chemical treatment. Incubation times and treatment length may need to be adjusted.

Western Blot Conditions

Recommended Gel Type: 4-12% Bis-Tris
Running Buffer: MES
Primary Concentration: 1/5000-1/10000 dilution
Primary Incubation: 4°C overnight or room temperature for 1 hour
Blocking Conditions: 5% BSA
Blocking Time: Room temperature for 1hr

Positive Controls: Cell line overexpressing PADI4 and treated with CaCl₂/Ionomycin
Negative controls: Cell line overexpressing PADI4

Lysate preparation protocol for western blotting | Abcam
Sample preparation of membrane-bound and nuclear proteins for western blot | Abcam
Western blot protocol for low molecular weight proteins
Troubleshooting tips for western blot | Abcam

ICC Conditions

Primary Concentration: 1/100 dilution
Primary Incubation: 4°C overnight or room temperature for 2 hours
Fixation: 4% PFA
Fixation time: 10-20 minutes at room temperature
Permeabilization: 0.1% Triton X-100
Permeabilization Time: 2-5 minutes at room temperature
Blocking: 2-10% serum from secondary antibody host e.g. ab7481 or ab7475
Blocking Time: 1-2 hours at room temperature

Positive Controls: Cell line overexpressing PADI4 and treated with CaCl₂/Ionomycin
Negative controls: Cell line overexpressing PADI4
Secondary only control:

Immunocytochemistry protocol | Abcam

FC Conditions

Primary Concentration: 1/500 dilution
Fixation: 4% PFA

Positive Controls: Cell line overexpressing PADI4 and treated with CaCl₂/Ionomycin
Negative controls: Cell line overexpressing PADI4

Flow Cytometry Protocol | Abcam
Flow cytometry troubleshooting - data optimization | Abcam

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 citrulline R8

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature 646:462-473 PubMed40836096

2025

Cancer-induced nerve injury promotes resistance to anti-PD-1 therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Erez N Baruch,Frederico O Gleber-Netto,Priyadharsini Nagarajan,Xiayu Rao,Shamima Akhter,Tuany Eichwald,Tongxin Xie,Mohammad Balood,Adebayo Adewale,Shorook Naara,Hinduja N Sathishkumar,Shajedul Islam,William McCarthy,Brandi J Mattson,Renata Ferrarotto,Michael K Wong,Michael A Davies,Sonali Jindal,Sreyashi Basu,Karine Roversi,Amin Reza Nikpoor,Maryam Ahmadi,Ali Ahmadi,Catherine Harwood,Irene Leigh,Dennis Gong,Paulino Tallón de Lara,Derrick L Tao,Tara M Davidson,Nadim J Ajami,Andrew Futreal,Kunal Rai,Veena Kochat,Micah Castillo,Preethi Gunaratne,Ryan P Goepfert,Sharia D Hernandez,Nikhil I Khushalani,Jing Wang,Stephanie S Watowich,George A Calin,Michael R Migden,Mona Yuan,Naijiang Liu,Yi Ye,William L Hwang,Paola D Vermeer,Nisha J D'Silva,Yuri L Bunimovich,Dan Yaniv,Jared K Burks,Javier Gomez,Patrick M Dougherty,Kenneth Y Tsai,James P Allison,Padmanee Sharma,Jennifer A Wargo,Jeffrey N Myers,Sebastien Talbot,Neil D Gross,Moran Amit

Nature communications 16:5393 PubMed40562767

2025

PADI4-mediated citrullination of histone H3 stimulates HIV-1 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Love,Bianca B Jütte,Birgitta Lindqvist,Hannah Rohdjess,Oscar Kieri,Piotr Nowak,J Peter Svensson

Heliyon 10:e37070 PubMed39296147

2024

AOC3 accelerates lung metastasis of osteosarcoma by recruiting tumor-associated neutrophils, neutrophil extracellular trap formation and tumor vascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Luxia Qi,Tian Gao,Chujie Bai,Zhanfei Guo,Linjing Zhou,Xiaodong Yang,Zhengfu Fan,Guifang Zhang

Cellular and molecular life sciences : CMLS 81:122 PubMed38456997

2024

Neutrophil extracellular traps mediate cardiomyocyte ferroptosis via the Hippo-Yap pathway to exacerbate doxorubicin-induced cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Zhao,You Li,Xiangli Xu,Haobo Yang,Xintong Li,Shuai Fu,Zihong Guo,Jianing Zhang,Hairu Li,Jiawei Tian

iScience 26:108289 PubMed38034352

2023

Neutrophil extracellular traps and DNases orchestrate formation of peritoneal adhesions.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Elrod,Annika Heuer,Jasmin Knopf,Janina Schoen,Lavinia Schönfeld,Magdalena Trochimiuk,Carolin Stiel,Birgit Appl,Laia Pagerols Raluy,Ceren Saygi,Leticija Zlatar,Sami Hosari,Dmytro Royzman,Thomas H Winkler,Günter Lochnit,Moritz Leppkes,Robert Grützmann,Georg Schett,Christian Tomuschat,Konrad Reinshagen,Martin Herrmann,Tobias A Fuchs,Michael Boettcher

Philosophical transactions of the Royal Society of London. Series B, Biological sciences 378:20230042 PubMed37778390

2023

Peptidylarginine deiminase 4 and ADAMTS13 activity in bacteraemia.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline P Martens,Marijke Peetermans,Thomas Vanassche,Peter Verhamme,Marc Jacquemin,Kimberly Martinod

Frontiers in immunology 14:1113948 PubMed36825027

2023

Murine scald models characterize the role of neutrophils and neutrophil extracellular traps in severe burns.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Elrod,Moritz Lenz,Antonia Kiwit,Lina Armbrust,Lavinia Schönfeld,Konrad Reinshagen,Laia Pagerols Raluy,Christoph Mohr,Ceren Saygi,Malik Alawi,Holger Rohde,Martin Herrmann,Michael Boettcher

Cells 11: PubMed36010543

2022

Deficiency Alters the Epigenomic Landscape and Causes Premature Differentiation of Mouse Trophoblast Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Noura N Ballasy,Elizabeth A Bering,Caroline Kokorudz,Bethany N Radford,Xiang Zhao,Wendy Dean,Myriam Hemberger

Frontiers in immunology 12:675315 PubMed34616390

2021

Early and Late Processes Driving NET Formation, and the Autocrine/Paracrine Role of Endogenous RAGE Ligands.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Tatsiy,Vanessa de Carvalho Oliveira,Hugo Tshivuadi Mosha,Patrick P McDonald

Cancer biology & medicine : PubMed33710810

2021

Analysis of the isoform-regulated transcriptome identifies as a downstream target in gastric carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Ni,Wenquan Lu,Qi Li,Chuan Han,Ting Yuan,Nina Sun,Yongquan Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (citrulline R8) antibody [EPR20358-13]

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Flow Cytometry (Intracellular) - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

ELISA - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Immunoprecipitation - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Western blot - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

ELISA - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Western blot - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Western blot - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

Product promise