AB214654

Anti-Histone H3 (di methyl K23) antibody [RM171]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal H3 di methyl K23 antibody. Suitable for ELISA, WB and reacts with Synthetic peptide, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C1 di methyl K23.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K23me2, H3K23me, H3K23

2 Images

ELISA - Anti-Histone H3 (di methyl K23) antibody [RM171] (AB214654)

ELISA

Supplier Data

ELISA - Anti-Histone H3 (di methyl K23) antibody [RM171] (AB214654)

ab214654 specifically reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross reactivity with other methylated lysines in Histone H3.

Supplier Data

Western blot - Anti-Histone H3 (di methyl K23) antibody [RM171] (AB214654)

All lanes:

Western blot - Anti-Histone H3 (di methyl K23) antibody [RM171] (ab214654) at 0.5 µg/mL

Lane 1:

Recombinant histone H3.3

Lane 2:

Acid extracts of HeLa cells

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM171

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ELISA

applications

Immunogen

Synthetic Peptide within Human H3C1 di methyl K23. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

ab214654 reacts to Histone H3 dimethylated at Lysine 23. There is no cross reactivity with nonmodified Lysine 23, monomethylated Lysine 23 (K23me1), or other methylations in histone H3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab214654 was purified from an animal origin-free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 di methyl K23

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:480 PubMed40164758

2025

Protein covariation networks for elucidating ferroptosis inducer mechanisms and potential synergistic drug targets.

Applications

Unspecified application

Species

Unspecified reactive species

Rina Kunishige,Yoshiyuki Noguchi,Naomi Okamoto,Lei Li,Akito Ono,Masayuki Murata,Fumi Kano

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (di methyl K23) antibody [RM171]

ELISA - Anti-Histone H3 (di methyl K23) antibody [RM171] (AB214654)

Western blot - Anti-Histone H3 (di methyl K23) antibody [RM171] (AB214654)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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