AB194690

Anti-Histone H3 (di methyl K27) antibody - ChIP Grade

Name: Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (ab194690) | Abcam
Brand: Abcam Limited
SKU: AB194690
Price: 515 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal H3 di methyl K27 antibody. Suitable for ChIP, Dot, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat, Synthetic peptide, Recombinant full length protein - Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human H3-4 di methyl K27.

View Alternative Names

H3FT, HIST3H3, H3-4, Histone H3.1t, H3/t, H3t, H3/g, Histone H3.4, H3K27me2, H3K27me, H3K27

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Paraffin-embedded human kidney cancer tissue stained for Histone H3 (di methyl K27) using ab194690 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Immunofluorescence staining of 293T cells stained for Histone H3 (di methyl K27) with ab194690. Nuclei are labeled with DAPI (Blue).

ChIP - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

ChIP

Supplier Data

ChIP - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Chromatin immunoprecipitation analysis of extracts of 293T cell line, using ab194690 and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Paraffin-embedded rat brain tissue stained for Histone H3 (di methyl K27) using ab194690 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Paraffin-embedded mouse heart tissue stained for Histone H3 (di methyl K27) using ab194690 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (ab194690)

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

Histone H3 protein expressed in E.coli at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 15 kDa

false

Supplier Data

Dot Blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Dot-blot analysis of methylation peptides using ab194690 at 1 : 1000 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ChIP, WB, IHC-P, ICC/IF, Dot

applications

Immunogen

Synthetic Peptide within Human H3-4 di methyl K27. The exact immunogen used to generate this antibody is proprietary information.

Q16695

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1/20 - 1/100", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3-4 di methyl K27

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37298094

2023

Epigenetic Histone Methylation of PPARγ and CPT1A Signaling Contributes to Betahistine Preventing Olanzapine-Induced Dyslipidemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqing Su,Chao Deng,Xuemei Liu,Jiamei Lian

FEBS letters 593:2556-2565 PubMed31254363

2019

Protective effect of histone methyltransferase NSD3 on ISO-induced cardiac hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Chen,Dongdong Jian,Linwei Zhao,Xiaobiao Zang,Weifeng Song,Jifang Ma,Zhen Jia,Xianqing Wang,Chuanyu Gao

Clinical epigenetics 9:95 PubMed28878842

2017

Efficacy of EZH2 inhibitory drugs in human papillomavirus-positive and human papillomavirus-negative oropharyngeal squamous cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Cameron D Lindsay,Morris A Kostiuk,Jeff Harris,Daniel A O'Connell,Hadi Seikaly,Vincent L Biron

Cell stem cell 20:274-289.e7 PubMed27939217

2016

PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.

Applications

Species

Human

Zhexin Zhu,Chunliang Li,Yanwu Zeng,Jianyi Ding,Zepeng Qu,Junjie Gu,Laixiang Ge,Fan Tang,Xin Huang,Chenlin Zhou,Ping Wang,Deyou Zheng,Ying Jin

Molecular medicine reports 12:7561-7 PubMed26459745

2015

Epigenetic modifications of histone H3 during the transdifferentiation of Thy-1(+) Lin(‑) bone marrow cells into hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Liao,Yixin Liao,Yantai Zou,Guanhong Li,Caixian Liao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (di methyl K27) antibody - ChIP Grade

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

ChIP - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Dot Blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB194690)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise