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AB24684

Anti-Histone H3 (di methyl K27) antibody - ChIP Grade

5

(26 Reviews)

|

(123 Publications)

Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (ab24684) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ChIP. Suitable for Caenorhabditis elegans, Cow, Human, Mouse.

- Over 100 publications
- Trusted since 2005

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27me2, H3K27me, H3K27

5 Images
Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB24684)
  • WB

Unknown

Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB24684)

The peptide competition assay shows that this antibody is very specific for Histone H3 di-methylated at K27. It does not recognise Histone H3 mono, di, or tri methylated at K4, Histone H3 mono, di, or tri methylated at K9, or Histone H3 mono, or tri methylated at K27.

All lanes:

Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (ab24684) at 1 µg/mL

Lanes 1, 3, 4, 5, 6, 7, 8, 9, 10 and 11:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Human Histone H3 peptide (<a href='/en-us/products/unavailable/human-histone-h3-peptide-ab17163'>ab17163</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG H&L (HRP) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 5s

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB24684)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB24684)

ab24684 staining Histone H3 (di methyl K27) in HeLa cells. The cells were fixed with 100% Methanol (5min), permeabilized with 0.1%PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab24684 at 1μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB24684)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB24684)

IHC image of Histone H3 (di methyl K27) staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24684, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB24684)
  • ICC/IF

AbReview39295****

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB24684)

Immunocytochemistry/ Immunofluorescence analysis of IMR32, Human Neuroblatoma cells labeling Histone H3 (di methyl K27) with ab24684 at 1/100 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.3% triton X 100. The cells were blocked with 1% BSA for 1 hour at 25°C, followed by incubation with primary antibody in 1% BSA containing TBST for 16 hours at 4°C. A Goat Anti Rabbit-IgG Cy3® was used as the secondary antibody at 1/1000 dilution.

This image is courtesy of an anonymous abreview.

ChIP - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB24684)
  • ChIP

Unknown

ChIP - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (AB24684)

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab24684 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Cow, Human, Caenorhabditis elegans

Applications

ICC/IF, ChIP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific for Histone H3 di-methylated at residue K27. It does not recognise the mono- or tri-methylated K27 residue or the mono-/di-/tri-methylated H3 K9 residue.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (ab24684) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in Caenorhabditis elegans, Cow, Human, Mouse samples.

What is the molecular weight of Histone H3?
Anti-Histone H3 (di methyl K27) - ChIP Grade (ab24684) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15.2kDa.

Trusted by the scientific community
Anti-Histone H3 (di methyl K27) - ChIP Grade (ab24684) was first used in a scientific publication in 2005 and has been cited over 100 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H3 (di methyl K27) - ChIP Grade (ab24684) has over 25 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 di methyl K27
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Publications (123)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2264-2275 PubMed40890361

2025

Chromatin dynamics of a large-sized genome provides insights into polyphenism and X0 dosage compensation of locusts.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Liu,Feng Jiang,Ran Li,Shanlin Liu,Wenjing Fang,Xiao Li,Ting Hu,Lianyun Feng,Jie Zhang,Zhikang Liu,Jing He,Wei Guo,Xianhui Wang,Zongyi Sun,Jingjing Li,Yunan Gao,Jiacheng Yi,Qiye Li,Xiaoxiao Wang,Liya Wei,Le Kang

Cell death & disease 16:553 PubMed40701963

2025

H3K14 lactylation exacerbates neuronal ferroptosis by inhibiting calcium efflux following intracerebral hemorrhagic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Sun,Jian-Nan Zhang,Ting Lan,Lei Shi,Liye Hu,Liyan Yan,Chao Wei,Lisha Hei,Weihua Wu,Zhaoli Luo,Meng Liu,Xingmei Ren,Yamei Wang,Yabin Lu,Peipei Wang,Chenguang Zhang,Liheng Bian,Xingquan Zhao,Fei Yang,Qian Li

PLoS genetics 21:e1011664 PubMed40305565

2025

Heat inducible nuclear translocation of Kdm6bb drives temperature dependent sex reversal in Nile tilapia.

Applications

Unspecified application

Species

Unspecified reactive species

Jigang Lu,Siqi Huang,Shicen Wei,Jiangbo Cheng,Wei Li,Yueyue Fei,Jihui Yang,Ruiqin Hu,Songqian Huang,Wanying Zhai,Zhichao Wu,Mingli Liu,Qianghua Xu,Peng Hu,Liangbiao Chen

Molecular medicine (Cambridge, Mass.) 31:109 PubMed40114103

2025

Perinatal hypoxia-mediated neurodevelopment abnormalities in congenital heart disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Renwei Chen,Haifan Wang,Liqin Zeng,Jiafei He,Xiaohan Liu,Xinting Ji,Paul Yao,Shuo Gu

PloS one 19:e0309471 PubMed39570878

2024

Immunohistochemical study of histone protein 3 modification in pediatric osteosarcoma identifies reduced H3K27me3 as a marker of poor treatment response.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Kondratowski,Danielle Cohen,Rebecca J Deyell,Ash Sandhu,Jonathan W Bush

Nature communications 15:5470 PubMed38937441

2024

Temperature-dependent jumonji demethylase modulates flowering time by targeting H3K36me2/3 in Brassica rapa.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyun Xin,Peirong Li,Xiuyun Zhao,Yangjun Yu,Weihong Wang,Guihua Jin,Jiao Wang,Liling Sun,Deshuang Zhang,Fenglan Zhang,Shuancang Yu,Tongbing Su

Signal transduction and targeted therapy 9:65 PubMed38461173

2024

Tet methylcytosine dioxygenase 2 (TET2) deficiency elicits EGFR-TKI (tyrosine kinase inhibitors) resistance in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhang,Kejia Zhao,Wenjing Zhou,Ran Kang,Shiyou Wei,Yueli Shu,Cheng Yu,Yin Ku,Yonghong Mao,Hao Luo,Juqin Yang,Jiandong Mei,Qiang Pu,Senyi Deng,Zhengyu Zha,Gang Yuan,Shensi Shen,Yaohui Chen,Lunxu Liu

Cell death and differentiation 31:106-118 PubMed38012390

2023

H3K36 methyltransferase NSD1 protects against osteoarthritis through regulating chondrocyte differentiation and cartilage homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Shao,Jinlong Suo,Zhong Zhang,Mingxiang Kong,Yiyang Ma,Yang Wen,Mengxue Liu,Lenan Zhuang,Kai Ge,Qing Bi,Changqing Zhang,Weiguo Zou

Insect molecular biology 33:457-466 PubMed37702080

2023

The diapausing mosquito Culex pipiens exhibits reduced levels of H3K27me2 in the fat body.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyan Wei,Prabin Dhungana,Cheolho Sim

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2301367 PubMed37565374

2023

The Lysine Demethylase KDM7A Regulates Immediate Early Genes in Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Wang,Qin Hong,Yueyue Xia,Zhao Zhang,Bo Wen
View all publications
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