Rabbit Polyclonal H3 di methyl K36 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Caenorhabditis elegans, Mouse, Synthetic peptide samples. Immunogen corresponding to Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10. The exact immunogen used to generate this antibody is proprietary information. Database link Q71DI3
Reactivity data
Select an application| ChIP | Dot | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Expected |
| Mouse | Expected | Expected | Expected | Tested |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Caenorhabditis elegans | Expected | Expected | Tested | Expected |
| Chicken | Predicted | Predicted | Predicted | Predicted |
| Drosophila melanogaster | Predicted | Predicted | Predicted | Predicted |
| Synthetic peptide | Not recommended | Tested | Not recommended | Not recommended |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-5 µg/cells for 6 µg/cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Drosophila melanogaster | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Caenorhabditis elegans, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Drosophila melanogaster | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species Caenorhabditis elegans | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Drosophila melanogaster | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Caenorhabditis elegans | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Drosophila melanogaster | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Additional Targets
H3C1 di methyl K36, H3C1 di methyl K36
Alternative names
HIST2H3A, H3C14, H3F2, H3FM, HIST2H3C, H3C13, HIST2H3D, H3C15, Histone H3.2, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, Histone H3/m, Histone H3/o, H3K36me2
Recommended products
Rabbit Polyclonal H3 di methyl K36 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Caenorhabditis elegans, Mouse, Synthetic peptide samples. Immunogen corresponding to Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10. The exact immunogen used to generate this antibody is proprietary information. Database link Q71DI3
- Purification technique
- Affinity purification Immunogen
- Specificity
A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
- Concentration
- Purification notes
Anti-Histone H3 [Dimethyl Lys36] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
ChIP - Anti-Histone H3 (di methyl K36) antibody (ab272158)
Chromatin Immunoprecipitation with ab272158. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of ab272158 and 20 μl of magnetic IgG beads per immunoprecipitation. A "no antibody" (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
Western blot - Anti-Histone H3 (di methyl K36) antibody (ab272158)
All lanes: Western blot - Anti-Histone H3 (di methyl K36) antibody (ab272158) at 1/500 dilution
All lanes: HeLa histone preps at 30 µg
SecondaryAll lanes: IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K36) antibody (ab272158)
Immunofluorescence of 0.5% PFA-fixed Neuro-2a (mouse neuroblastoma cell line) cells labeling Histone H3 (di methyl K36) with ab272158 at 1/100 dilution for 1 hour at room temperature, followred by FITC secondary antibody at 1/10000 for 45 minutes at room temperature. Histone H3 (di methyl K36) staining is green. Nuclei were counterstained with DAPI (blue).
Western blot - Anti-Histone H3 (di methyl K36) antibody (ab272158)
All lanes: Western blot - Anti-Histone H3 (di methyl K36) antibody (ab272158) at 1/500 dilution
All lanes: C.elegans embryo lysate at 30 µg
SecondaryAll lanes: IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
Dot Blot - Anti-Histone H3 (di methyl K36) antibody (ab272158)
Dot Blot of ab272158.
Lane 1: K36.
Lane 2: K36Me1.
Lane 3: K36Me2.
Lane 4: K36Me3.
Lane 5: K36Ac.
Load: 1, 3, 6, and 10 picomoles of peptide. Primary antibody ab272158 at 1/1000 for 45 minutes at 4°C. Dylight™488 rabbit secondary antibody at 1/10000 for 45 minutes at room temperature.
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