AB272158

Anti-Histone H3 (di methyl K36) antibody

Name: Anti-Histone H3 (di methyl K36) antibody (ab272158) | Abcam
Brand: Abcam Limited
SKU: AB272158
Price: 640 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal H3 di methyl K36 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Caenorhabditis elegans, Mouse, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10.

View Alternative Names

HIST2H3A, H3C14, H3F2, H3FM, HIST2H3C, H3C13, HIST2H3D, H3C15, Histone H3.2, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, Histone H3/m, Histone H3/o, H3K36me2, H3K36me, H3K36

5 Images

ChIP - Anti-Histone H3 (di methyl K36) antibody (AB272158)

ChIP

Supplier Data

ChIP - Anti-Histone H3 (di methyl K36) antibody (AB272158)

Chromatin Immunoprecipitation with ab272158. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of ab272158 and 20 μl of magnetic IgG beads per immunoprecipitation. A "no antibody" (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K36) antibody (AB272158)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K36) antibody (AB272158)

Immunofluorescence of 0.5% PFA-fixed Neuro-2a (mouse neuroblastoma cell line) cells labeling Histone H3 (di methyl K36) with ab272158 at 1/100 dilution for 1 hour at room temperature, followred by FITC secondary antibody at 1/10000 for 45 minutes at room temperature. Histone H3 (di methyl K36) staining is green. Nuclei were counterstained with DAPI (blue).

Supplier Data

Western blot - Anti-Histone H3 (di methyl K36) antibody (AB272158)

All lanes:

Western blot - Anti-Histone H3 (di methyl K36) antibody (ab272158) at 1/500 dilution

All lanes:

HeLa histone preps at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-Histone H3 (di methyl K36) antibody (AB272158)

All lanes:

Western blot - Anti-Histone H3 (di methyl K36) antibody (ab272158) at 1/500 dilution

All lanes:

C.elegans embryo lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Supplier Data

Dot Blot - Anti-Histone H3 (di methyl K36) antibody (AB272158)

Dot Blot of ab272158.

Lane 1 : K36.

Lane 2 : K36Me1.

Lane 3 : K36Me2.

Lane 4 : K36Me3.

Lane 5 : K36Ac.

Load : 1, 3, 6, and 10 picomoles of peptide. Primary antibody ab272158 at 1/1000 for 45 minutes at 4°C. Dylight™488 rabbit secondary antibody at 1/10000 for 45 minutes at room temperature.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Caenorhabditis elegans

Applications

ChIP, WB, ICC/IF, Dot

applications

Immunogen

Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10. The exact immunogen used to generate this antibody is proprietary information.

Q71DI3

Specificity

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-5 µg/cells for 6 µg/cells", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Rat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Anti-Histone H3 [Dimethyl Lys36] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C15 di methyl K36

Additional targets

,H3C1 di methyl K36

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:11059-11070 PubMed33149618

2020

The Pseudogene DUXAP8 Promotes Colorectal Cancer Cell Proliferation, Invasion, and Migration by Inducing Epithelial-Mesenchymal Transition Through Interacting with EZH2 and H3K27me3.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing He,Yi Yu,Wei Huang,Guoliang Feng,Junhe Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (di methyl K36) antibody

ChIP - Anti-Histone H3 (di methyl K36) antibody (AB272158)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K36) antibody (AB272158)

Western blot - Anti-Histone H3 (di methyl K36) antibody (AB272158)

Western blot - Anti-Histone H3 (di methyl K36) antibody (AB272158)

Dot Blot - Anti-Histone H3 (di methyl K36) antibody (AB272158)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (1)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise