AB9049

Anti-Histone H3 (di methyl K36) antibody - ChIP Grade

Name: Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (ab9049) | Abcam
Brand: Abcam Limited
SKU: AB9049
Price: 430 USD
Availability: InStock
Rating: 5 (24 reviews)

BOND RX™ Validated
What is this?

(24 Reviews)

(178 Publications)

Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (ab9049) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ChIP. Suitable for Cow, Drosophila melanogaster, Human.

- Over 140 publications
- Trusted since 2003

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K36me2, H3K36me, H3K36

4 Images

ChIP - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

ChIP

Unknown

ChIP - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 2 μg of ab9049(blue), and 20 μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH and RPL30 (active) promoters and over the g-Actin gene (active). Schematic diagram of the g-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

IHC image of ab9049 staining Histone H3 (di methyl K36) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9049, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

AbReview25030****

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

All lanes:

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (ab9049) at 1/1400 dilution

All lanes:

Whole cell lysate prepared from Drosophila BG3 tissue cells at 50000 Cells

Secondary

All lanes:

Donkey anti-rabbit polyclonal conjugated to HRP at 1/20000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa,42 kDa

true

Exposure time: 30s

Image courtesy of an anonymous Abreview.

Project1016****

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

All lanes:

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (ab9049) at 1 µg/mL

Lanes 1, 4, 5, 6, 7, 8, 9, 10 and 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H3 peptide (ab41430)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K36) peptide (<a href='/en-us/products/unavailable/human-histone-h3-mono-methyl-k36-peptide-ab1783'>ab1783</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Cow, Human, Drosophila melanogaster

Applications

ChIP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific for histone H3 di-methylated at K36. It does not react with mono or tri methyl K36.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-5 µg for 25 µg chromatin", "ChIP-species-notes": "Use RPL30 ChIP primer pair <a href='/en-us/products/reagents/rpl30-positive-control-chip-primer-pair-ab269262'>ab269262</a> as positive control.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Arabidopsis thaliana": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Caenorhabditis elegans": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Dictyostelium discoideum": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mammals": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Saccharomyces cerevisiae": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (ab9049) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), ChIP in Cow, Drosophila melanogaster, Human samples.

What is the molecular weight of Histone H3?
Anti-Histone H3 (di methyl K36) - ChIP Grade (ab9049) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa.

Trusted by the scientific community
Anti-Histone H3 (di methyl K36) - ChIP Grade (ab9049) was first used in a scientific publication in 2003 and has been cited over 140 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H3 (di methyl K36) - ChIP Grade (ab9049) has over 20 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 di methyl K36

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Publications (178)

Recent publications for all applications. Explore the full list and refine your search

aBIOTECH 6:411-423 PubMed40994437

2025

Epigenetically poised chromatin states regulate and genes in soybean.

Applications

Unspecified application

Species

Unspecified reactive species

Linzhe Jin,Yihan Zhang,Jiayuan Guo,Xuexia Liu,Yanling Lai,Xinfang Huang,Yuhan Zou,Shichuang Yan,Xianzhe Dai,Zhenhui Zhong

Blood neoplasia 2:100091 PubMed40949769

2025

Discovery of a novel class NSD2 inhibitor for multiple myeloma with t(4;14).

Applications

Unspecified application

Species

Unspecified reactive species

Sae Matsuoka,Naoki Osada,Hirokazu Kubota,Ko Kikuzato,Hiroo Koyama,Takeshi Sonoda,Akiko Idei,Minoru Yoshida,Masaki Kikuchi,Takashi Umehara,Chiduru Watanabe,Teruki Honma,Hiroshi Yasui,Sho Ikeda,Naoto Takahashi,Hideki Nakasone,Jiro Kikuchi,Yusuke Furukawa

JCI insight 10: PubMed40663488

2025

NSD1-916aa encoded by CircNSD1 contributes to AKI-to-CKD transition through inducing ferroptosis in tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li Gao,Junsheng Zhang,Chaoyi Chen,Sai Zhu,Xianglong Wei,Guiqin Tang,Sheng Wang,Yukai Wang,Xinran Liu,Ling Jiang,Yonggui Wu

Nature communications 16:4681 PubMed40394007

2025

Histone methyltransferase ASH1L primes metastases and metabolic reprogramming of macrophages in the bone niche.

Applications

Unspecified application

Species

Unspecified reactive species

Chenling Meng,Kevin Lin,Wei Shi,Hongqi Teng,Xinhai Wan,Anna DeBruine,Yin Wang,Xin Liang,Javier Leo,Feiyu Chen,Qianlin Gu,Jie Zhang,Vivien Van,Kiersten L Maldonado,Boyi Gan,Li Ma,Yue Lu,Di Zhao

PLoS genetics 21:e1011689 PubMed40315272

2025

Centromere-size reduction and chromatin state dynamics following intergenomic hybridization in cotton.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlei Han,Guanjing Hu,Yan Dai,Xin Zhang,Jingjing Tian,Jialiang Zhou,Xinqi Xu,Qi Chen,Xiaobing Kou,Lei Xu,Xinyu Wu,Ziying Sun,Jiahui Geng,Lin Li,Chenyu Qiu,Teame Gereziher Mehari,Baohua Wang,Hui Zhang,Xinlian Shen,Zhenzhen Xu,Jonathan F Wendel,Kai Wang

PLoS genetics 21:e1011664 PubMed40305565

2025

Heat inducible nuclear translocation of Kdm6bb drives temperature dependent sex reversal in Nile tilapia.

Applications

Unspecified application

Species

Unspecified reactive species

Jigang Lu,Siqi Huang,Shicen Wei,Jiangbo Cheng,Wei Li,Yueyue Fei,Jihui Yang,Ruiqin Hu,Songqian Huang,Wanying Zhai,Zhichao Wu,Mingli Liu,Qianghua Xu,Peng Hu,Liangbiao Chen

Nature communications 16:2235 PubMed40044670

2025

Structure-function relationship of ASH1L and histone H3K36 and H3K4 methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Kendra R Vann,Rajal Sharma,Chih-Chao Hsu,Maeva Devoucoux,Adam H Tencer,Lei Zeng,Kevin Lin,Li Zhu,Qin Li,Catherine Lachance,Ruben Rosas Ospina,Qiong Tong,Ka Lung Cheung,Shuai Yang,Soumi Biswas,Hongwen Xuan,Jovylyn Gatchalian,Lorena Alamillo,Jianlong Wang,Suk Min Jang,Brianna J Klein,Yue Lu,Patricia Ernst,Brian D Strahl,Scott B Rothbart,Martin J Walsh,Michael L Cleary,Jacques Côté,Xiaobing Shi,Ming-Ming Zhou,Tatiana G Kutateladze

Cell reports 44:115213 PubMed39817906

2025

Coordinated neuron-specific splicing events restrict nucleosome engagement of the LSD1 histone demethylase complex.

Applications

Unspecified application

Species

Unspecified reactive species

Robert S Porter,Sojin An,Maria C Gavilan,Masayoshi Nagai,Yumie Murata-Nakamura,Bo Zhou,Katherine M Bonefas,Olivier Dionne,Jeru Manoj Manuel,Joannie St-Germain,Suzanne Gascon,Jacqueline Kim,Liam Browning,Benoit Laurent,Uhn-Soo Cho,Shigeki Iwase

Life science alliance 8: PubMed39741006

2025

NSD3 protein methylation and stabilization transforms human ES cells into variant state.

Applications

Unspecified application

Species

Unspecified reactive species

Vignesh K Krishnamoorthy,Fariha Hamdani,Pooja Shukla,Radhika Arasala Rao,Shaikh Anaitullah,Kriti Kestur Biligiri,Rajashekar Varma Kadumuri,Purushotham Reddy Pothula,Sreenivas Chavali,Shravanti Rampalli

Genetics in medicine open 2:101838 PubMed39669601

2024

Loss of function in causes DNA methylation signature similar to that in Wolf-Hirschhorn syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoko Kawai,Shiori Kinoshita,Yuka Takayama,Eriko Ohnishi,Hiromi Kamura,Kazuaki Kojima,Hiroki Kikuchi,Miho Terao,Tohru Sugawara,Ohsuke Migita,Masayo Kagami,Tsuyoshi Isojima,Yu Yamaguchi,Keiko Wakui,Hirofumi Ohashi,Kenji Shimizu,Seiji Mizuno,Nobuhiko Okamoto,Yoshimitsu Fukushima,Fumio Takada,Kenjiro Kosaki,Shuji Takada,Hidenori Akutsu,Kiyoe Ura,Kazuhiko Nakabayashi,Kenichiro Hata

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (di methyl K36) antibody - ChIP Grade

ChIP - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (AB9049)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (178)

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