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Rabbit Recombinant Monoclonal H3 di methyl K4 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, IP, ChIP, WB, ICC/IF, ChIP-seq, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, African green monkey, Chicken, Cow samples.

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Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Shipping To:
United States

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Protein ArrayChIC/CUT&RUN-seqIPChIPWBICC/IFChIP-seqFlow Cyt (Intra)IHC-P
Human
Not recommended
Tested
Tested
Tested
Tested
Tested
Tested
Tested
Tested
Mouse
Not recommended
Expected
Expected
Expected
Tested
Expected
Expected
Expected
Tested
Rat
Not recommended
Expected
Expected
Expected
Tested
Expected
Expected
Expected
Tested
African green monkey
Not recommended
Expected
Expected
Expected
Tested
Expected
Expected
Expected
Expected
Chicken
Not recommended
Expected
Expected
Expected
Tested
Expected
Expected
Expected
Expected
Cow
Not recommended
Expected
Expected
Expected
Tested
Expected
Expected
Expected
Expected
Monkey
Not recommended
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Rice
Not recommended
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted

Images

Not recommended
Not recommended

Species
Mouse, Rat, Chicken, Cow, Human, Monkey, African green monkey, Rice
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, African green monkey, Chicken, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Monkey, Rice
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat, African green monkey, Chicken, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Monkey, Rice
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat, African green monkey, Chicken, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Monkey, Rice
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species
Rat
Dilution info
-
Notes

-

Species
African green monkey
Dilution info
-
Notes

-

Species
Chicken
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

-

Species
Cow
Dilution info
-
Notes

-

Predicted
Predicted

Species
Monkey, Rice
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, African green monkey, Chicken, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Monkey, Rice
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
4 µg chromatin for 30.00000 µg chromatin
Notes

-

Expected
Expected

Species
Mouse, Rat, African green monkey, Chicken, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Monkey, Rice
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat, African green monkey, Chicken, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Monkey, Rice
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
African green monkey, Chicken, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Monkey, Rice
Dilution info
-
Notes

-

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Rabbit Recombinant Monoclonal H3 di methyl K4 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, IP, ChIP, WB, ICC/IF, ChIP-seq, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, African green monkey, Chicken, Cow samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Carrier free

Yes

Clone number

Y47

Purification technique

Affinity purification Protein A

Specificity

This antibody only detects Histone H3 dimethylated on Lysine 4.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab173324 is the carrier-free version of ab32356.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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