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Rabbit Recombinant Monoclonal H3 di methyl K4 antibody. Suitable for ChIC/CUT&RUN-seq, IP, ChIP, WB, ICC/IF, ChIP-seq, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, African green monkey, Chicken, Cow samples. Cited in 201 publications.
Alternative names=Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3J, H3FJ, H3FF, H3C12, H3C11, H3C1, H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, HIST1H3I, H3K4me2
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| PepArr | ChIC/CUT&RUN-seq | IP | ChIP | WB | ICC/IF | ChIP-seq | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested | Tested | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Expected | Expected | Expected | Tested | Expected | Expected | Expected | Tested |
| Rat | Not recommended | Expected | Expected | Expected | Tested | Expected | Expected | Expected | Tested |
| African green monkey | Not recommended | Expected | Expected | Expected | Tested | Expected | Expected | Expected | Expected |
| Arabidopsis thaliana | Not recommended | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Chicken | Not recommended | Expected | Expected | Expected | Tested | Expected | Expected | Expected | Expected |
| Cow | Not recommended | Expected | Expected | Expected | Tested | Expected | Expected | Expected | Expected |
| Mammals | Not recommended | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Monkey | Not recommended | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rice | Not recommended | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Saccharomyces cerevisiae | Not recommended | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Sheep | Not recommended | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Xenopus laevis | Not recommended | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Zebrafish | Not recommended | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Images
PepArr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Chicken, Cow, Human, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Monkey, Zebrafish, Mammals, African green monkey, Rice | Dilution info - | Notes - |
ChIC/CUT&RUN-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey, Chicken, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Monkey, Zebrafish, Mammals, Rice | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey, Chicken, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Monkey, Zebrafish, Mammals, Rice | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg chromatin for 25 µg chromatin | Notes Use ALDOA ChIP primer pair ab269260 as positive control. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey, Chicken, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Monkey, Zebrafish, Mammals, Rice | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/2000 | Notes - |
Species African green monkey | Dilution info 1/2000 | Notes - |
Species Chicken | Dilution info 1/2000 | Notes - |
Species Human | Dilution info 1/2000 | Notes - |
Species Cow | Dilution info 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Monkey, Zebrafish, Mammals, Rice | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey, Chicken, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Monkey, Zebrafish, Mammals, Rice | Dilution info - | Notes - |
ChIP-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg chromatin for 30 µg chromatin | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey, Chicken, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Monkey, Zebrafish, Mammals, Rice | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey, Chicken, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Monkey, Zebrafish, Mammals, Rice | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/800 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/800 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/800 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey, Chicken, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Monkey, Zebrafish, Mammals, Rice | Dilution info - | Notes - |
Recommended products
Rabbit Recombinant Monoclonal H3 di methyl K4 antibody. Suitable for ChIC/CUT&RUN-seq, IP, ChIP, WB, ICC/IF, ChIP-seq, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, African green monkey, Chicken, Cow samples. Cited in 201 publications.
Alternative names=Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3J, H3FJ, H3FF, H3C12, H3C11, H3C1, H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, HIST1H3I, H3K4me2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Clone number
Y47
- Purification technique
Affinity purification Protein A
- Specificity
This antibody only detects Histone H3 dimethylated on Lysine 4.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Every new batch of this antibody is tested in house in ChIP.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
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