AB32356

Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade

Name: Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (ab32356) | Abcam
Brand: Abcam Limited
SKU: AB32356
Price: 140 USD
Availability: InStock
Rating: 5 (14 reviews)

20ul selling size
RabMAb
Advanced Validation
Recombinant
What is this?

(14 Reviews)

(254 Publications)

Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (ab32356) is a rabbit monoclonal antibody detecting Histone H3 in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF, ChIP. Suitable for African green monkey, Chicken, Cow, Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 200 publications
- Trusted since 2006

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me2, H3K4me, H3K4

17 Images

Flow Cytometry (Intracellular) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Intracellular Flow Cytometry analysis of HeLa cells labelling Histone H3 (di methyl K4) with purified ab32356 at 1/150 (red). Cells were fixed with 80% methanol. An Alexa Fluorr^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG (ab172730). Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinomal) cells labeling Histone H3 di methyl K4 with purified ab32356 at 1/500 dilution (1.17μg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Overlay histogram showing HeLa cells stained with unpurified ab32356 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32356, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit DyLight^® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Histone H3 (di methyl K4) with purified ab32356 at a dilution of 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab7291, a mouse anti-tubulin (1/1000) using ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary antibody. Nuclei counterstained with DAPI (blue).

Control 1 : primary antibody (1/1000) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling Histone H3 (di methyl K4) with purified ab32356 at a dilution of 1/800. Antigen retrieval was performed using Tris/EDTA buffer, pH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody (1/500). Counter stained with hematoxylin.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

ICC/IF image of unpurified ab32356 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32356 , 5μg/ml) overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (IgG - H&L, pre-adsorbed) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

ChIP - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

ChIP

Unknown

ChIP - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab32356 (red), and 20μl of Anti Rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.

ChIP-sequencing - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

ChIP-seq

Supplier Data

ChIP-sequencing - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (ab32356). ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed by Active Motif, Carlsbad, CA.

Additional screenshots of mapped reads can be downloaded here.

ChIP-seq

Lab

ChIP-sequencing - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 4 μg of Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (ab32356). ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

Additional screenshots of mapped reads can be downloaded here.

Lab

Immunoprecipitation - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

ab32356 (purified) at 1/30 dilution (20 μg/ml) immunoprecipitating Histone H3 di methyl K4 in HepG2 whole cell lysate.

Lane 1 (input) : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab32356 & HepG2 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32356 in HepG2 whole cell lysate
For western blotting, ab32356 at 1/500 and veriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.

Blocking and diluting buffer : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (ab32356)

Predicted band size: 15 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling Histone H3 (di methyl K4) with purified ab32356 at a dilution of 1/800. Antigen retrieval was performed using Tris/EDTA buffer, pH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody (1/500). Counter stained with hematoxylin.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue labelling Histone H3 (di methyl K4) with purified ab32356 at a dilution of 1/800. Antigen retrieval was performed using Tris/EDTA buffer, pH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody (1/500). Counter stained with hematoxylin.

Lab

Western blot - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

This blot was produced using 14% SDS-PAGE containing 15 μg of HT-29 whole cell lysate per lane at 150V for 50 minutes before being transferred onto a 0.45 μm PVDF membrane at 72V for 50 minutes. The membrane was then blocked for 1hr using 5% NFDM/TBST, then incubated with ab32356 (1/10,000) at room temperature for 1hr. After being washed three times in TBST, the membrane was incubated with Peroxidase conjugated goat anti-rabbit IgG (H+L) (ab97051) at 1/20,000 dilution for 1hr at room temperature. The membrane was washed three times again. Then the signal was developed using the ECL technique.

ab32356 was stored at a range of temperatures (+4°C, +22°C, +37°C) for 1 week before being tested in WB. The image shows the band intensity remains relatively constant across all storage temperatures, demonstrating that antibody activity is not affected under these conditions. This data was generated as part of a structured antibody stability study supporting Abcam’s transition from cold‑chain to ambient shipping.

All lanes:

Western blot - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (ab32356) at 1/10000 dilution

All lanes:

HT-29 whole cell lysate at 15 µg with NDFM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 20s

ChIC/CUT&RUN sequencing - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 5 µg of ab32356 [Y47]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. Additional screenshots of mapped reads can be found in the Protocol booklet in the Product Protocol section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

Lab

Western blot - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

All lanes:

Western blot - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (ab32356) at 1/10000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 3:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

Lane 4:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 5s

Lab

Western blot - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

All lanes:

Western blot - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (ab32356) at 1/2000 dilution

Lane 1:

L6 (Rat skeletal muscle cell line) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 3:

COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 20 µg

Lane 4:

UMNSAH/DF-1 (Transformed chicken embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 5:

MDBK (Bovine kidney cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 5s

Peptide Array - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

PepArr

Lab

Peptide Array - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

ab32356 or ab176878 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity. Peptide Array, ab32356 binds to Histone H3 (di methyl K4) on Peptide Array but shows some weak non-specific binding to other Histone H3 modifications. We recommend ab176878 for Peptide Array of Histone H3 (di methyl K4).

Carrier free

Anti-Histone H3 (di methyl K4) antibody [Y47] - BSA and Azide free
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-Histone H3 (di methyl K4) antibody [Y47]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Histone H3 (di methyl K4) antibody [Y47]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Histone H3 (di methyl K4) antibody [Y47]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Histone H3 (di methyl K4) antibody [Y47]
578 PE

PE Anti-Histone H3 (di methyl K4) antibody [Y47]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y47

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Chicken, Cow, Human, African green monkey

Applications

ChIC/CUT&RUN-seq, IHC-P, ChIP-seq, ChIP, ICC/IF, IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

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"predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2 µg for 25 µg chromatin", "ChIP-species-notes": "Use ALDOA ChIP primer pair <a href='/en-us/products/reagents/aldoa-positive-control-chip-primer-pair-ab269260'>ab269260</a> as positive control.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "4 µg for 30 µg chromatin", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "African green monkey": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Arabidopsis thaliana": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mammals": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rice": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Saccharomyces cerevisiae": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": 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"IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide - Human": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "notRecommended", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "PepArr-species-checked": "notRecommended", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", 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Product details

What is this antibody validated in?
Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (ab32356) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in African green monkey, Chicken, Cow, Human, Mouse, Rat samples.

What is the molecular weight of Histone H3?
Anti-Histone H3 (di methyl K4) [Y47] - ChIP Grade (ab32356) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa.

Trusted by the scientific community
Anti-Histone H3 (di methyl K4) [Y47] - ChIP Grade (ab32356) was first used in a scientific publication in 2006 and has been cited over 200 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H3 (di methyl K4) [Y47] - ChIP Grade (ab32356) has over 10 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [Y47] also available for your convenience: ab32356, Carrier free - ab173324, Alexa Fluor® 488 - ab200024, Alexa Fluor® 647 - ab200026, Alexa Fluor® 594 - ab207864, Alexa Fluor® 405 - ab207865, PE - ab208741

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting
Download chicCutRunSequencingBooklet|en
Download chipSequencingBookletWebsite|en

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 di methyl K4

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Publications (254)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2264-2275 PubMed40890361

2025

Chromatin dynamics of a large-sized genome provides insights into polyphenism and X0 dosage compensation of locusts.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Liu,Feng Jiang,Ran Li,Shanlin Liu,Wenjing Fang,Xiao Li,Ting Hu,Lianyun Feng,Jie Zhang,Zhikang Liu,Jing He,Wei Guo,Xianhui Wang,Zongyi Sun,Jingjing Li,Yunan Gao,Jiacheng Yi,Qiye Li,Xiaoxiao Wang,Liya Wei,Le Kang

Cell death & disease 16:553 PubMed40701963

2025

H3K14 lactylation exacerbates neuronal ferroptosis by inhibiting calcium efflux following intracerebral hemorrhagic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Sun,Jian-Nan Zhang,Ting Lan,Lei Shi,Liye Hu,Liyan Yan,Chao Wei,Lisha Hei,Weihua Wu,Zhaoli Luo,Meng Liu,Xingmei Ren,Yamei Wang,Yabin Lu,Peipei Wang,Chenguang Zhang,Liheng Bian,Xingquan Zhao,Fei Yang,Qian Li

European journal of immunology 55:e51680 PubMed40498295

2025

Butyrate Selectively Targets Super-Enhancers and Transcriptional Networks Associated with Human Mast Cell Function.

Applications

Unspecified application

Species

Unspecified reactive species

Jelle Folkerts,Marjolein J W de Bruijn,Wilfred F J van IJcken,Rudi W Hendriks,Ralph Stadhouders

Nature 642:1097-1105 PubMed40335694

2025

Chromatin loops are an ancestral hallmark of the animal regulatory genome.

Applications

Unspecified application

Species

Unspecified reactive species

Iana V Kim,Cristina Navarrete,Xavier Grau-Bové,Marta Iglesias,Anamaria Elek,Grygoriy Zolotarov,Nikolai S Bykov,Sean A Montgomery,Ewa Ksiezopolska,Didac Cañas-Armenteros,Joan J Soto-Angel,Sally P Leys,Pawel Burkhardt,Hiroshi Suga,Alex de Mendoza,Marc A Marti-Renom,Arnau Sebé-Pedrós

PLoS genetics 21:e1011664 PubMed40305565

2025

Heat inducible nuclear translocation of Kdm6bb drives temperature dependent sex reversal in Nile tilapia.

Applications

Unspecified application

Species

Unspecified reactive species

Jigang Lu,Siqi Huang,Shicen Wei,Jiangbo Cheng,Wei Li,Yueyue Fei,Jihui Yang,Ruiqin Hu,Songqian Huang,Wanying Zhai,Zhichao Wu,Mingli Liu,Qianghua Xu,Peng Hu,Liangbiao Chen

Cell biology and toxicology 41:52 PubMed40000487

2025

Molecular mechanism of ZC3H13 -mediated ferroptosis in doxorubicin resistance of triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li Huang,Lei Han,Shuai Liang,Guohui Han

Molecular medicine (Cambridge, Mass.) 31:65 PubMed39972431

2025

Mechanism of histone demethylase KDM5A in osteoporotic fracture healing through epigenetic regulation of the miR-495/SKP2/Runx2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoran Li,Junyan Zhang,Tingting Xu,Zhiying Hao,Yadong Li

Nature communications 16:1241 PubMed39890808

2025

Copy number amplification of FLAD1 promotes the progression of triple-negative breast cancer through lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Qing Song,Tian-Jian Yu,Yang Ou-Yang,Jia-Han Ding,Yi-Zhou Jiang,Zhi-Ming Shao,Yi Xiao

Genome research 34:2229-2243 PubMed39632087

2024

Characterization of DNA methylation reader proteins in .

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Cahn,James P B Lloyd,Ino D Karemaker,Pascal W T C Jansen,Jahnvi Pflueger,Owen Duncan,Jakob Petereit,Ozren Bogdanovic,A Harvey Millar,Michiel Vermeulen,Ryan Lister

Poultry science 104:104583 PubMed39616678

2024

miR-1458 is inhibited by low concentrations of Vitamin B6 and targets TBX6 to promote the formation of spermatogonial stem cells in Rugao Yellow Chicken.

Applications

Unspecified application

Species

Unspecified reactive species

Qingqing Geng,Cai Hu,Ziduo Zhao,Zhe Wang,Fufu Cheng,Jing Chen,Qisheng Zuo,Yani Zhang

View all publications

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chicCutRunSequencingBooklet

chipSequencingBookletWebsite

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade

Flow Cytometry (Intracellular) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Flow Cytometry (Intracellular) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Flow Cytometry (Intracellular) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

ChIP - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

ChIP-sequencing - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

ChIP-sequencing - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Immunoprecipitation - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Western blot - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

ChIC/CUT&RUN sequencing - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Western blot - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Western blot - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

Peptide Array - Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade (AB32356)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (254)

Product promise