Rabbit Recombinant Monoclonal H3 di methyl K56 antibody. Carrier free. Suitable for ICC/IF, WB, PepArr and reacts with Mouse, Human, Synthetic peptide - Human samples.
Images
![Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (AB249955), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-di-methyl-k56-antibody-epr176842-bsa-and-azide-free-ab249955--western-blot-img54423.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (AB249955)")
![Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (AB249955), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-di-methyl-k56-antibody-epr176842-bsa-and-azide-free-ab249955--western-blot-img16902.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (AB249955)")
![Peptide Array - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (AB249955), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-di-methyl-k56-antibody-epr176842-bsa-and-azide-free-ab249955--peptide-array-img30552.jpg/_jcr_content/renditions/webp-475.webp "Peptide Array - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (AB249955)")
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (AB249955), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-di-methyl-k56-antibody-epr176842-bsa-and-azide-free-ab249955--immunocytochemistry-immunofluorescence-img17182.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (AB249955)")
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (AB249955), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-di-methyl-k56-antibody-epr176842-bsa-and-azide-free-ab249955--immunocytochemistry-immunofluorescence-img12585.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (AB249955)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | PepArr | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Synthetic peptide - Human | Not recommended | Tested | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Mouse, Human | Dilution info - | Notes - |
PepArr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, HIST1H3C, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K56me2
Recommended products
Rabbit Recombinant Monoclonal H3 di methyl K56 antibody. Carrier free. Suitable for ICC/IF, WB, PepArr and reacts with Mouse, Human, Synthetic peptide - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17684(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249955 is the carrier-free version of Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-di-methyl-k56-antibody-epr176842-bsa-and-azide-free-ab249955--western-blot-img54423.jpg "Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)")
Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
This data was developed using Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% BSA/TBST.
Lane 1: Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] (Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294) at 1/5000 dilution
Lanes 2 - 4: Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] (Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294) at 1/1000 dilution
Lane 1: Recombinant mutant H3 chemically modified to mimic H3K56Me2 at 0.01 µg
Lane 2: Unmodified recombinant mutant H3 at 0.01 µg
Lane 3: Recombinant mutant H3 chemically modified to mimic H3K56Me1 at 0.01 µg
Lane 4: Recombinant mutant H3 chemically modified to mimic H3K56Me3 at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 3min
![Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-di-methyl-k56-antibody-epr176842-bsa-and-azide-free-ab249955--western-blot-img16902.jpg "Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)")
Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
This data was developed using Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] (Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294) at 1/200 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 3min
![Peptide Array - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-di-methyl-k56-antibody-epr176842-bsa-and-azide-free-ab249955--peptide-array-img30552.jpg "Peptide Array - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)")
Peptide Array - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
This data was developed using Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294, the same antibody clone in a different buffer formulation.
Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-di-methyl-k56-antibody-epr176842-bsa-and-azide-free-ab249955--immunocytochemistry-immunofluorescence-img17182.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
This data was developed using Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (di methyl K56) with Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red). The negative controls are as follows:
-ve control 1: Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294 at 1/150 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-di-methyl-k56-antibody-epr176842-bsa-and-azide-free-ab249955--immunocytochemistry-immunofluorescence-img12585.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
This data was developed using Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Histone H3 (di methyl K56) with Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red). The negative controls are as follows:
-ve control 1: Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] ab177294 at 1/150 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
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