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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Mouse Monoclonal H3 di methyl K9 antibody. Suitable for Dot, ICC/IF, ChIP, ELISA, WB, IHC-P and reacts with Human, Cow, Drosophila melanogaster, Arabidopsis thaliana, Rice samples. Cited in 961 publications.
IgG2a
Mouse
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Liquid
Monoclonal
Dot | ICC/IF | ChIP | ELISA | WB | IHC-P | |
---|---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Arabidopsis thaliana | Predicted | Predicted | Expected | Predicted | Predicted | Predicted |
Caenorhabditis elegans | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Chicken | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Common marmoset | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Corn | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Cow | Expected | Expected | Expected | Expected | Tested | Expected |
Drosophila melanogaster | Expected | Expected | Expected | Expected | Tested | Expected |
Rice | Predicted | Predicted | Expected | Predicted | Predicted | Predicted |
Saccharomyces cerevisiae | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Schizosaccharomyces pombe | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Sheep | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Xenopus laevis | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes ab1220 has some cross-reactivity with H3R8me2 in peptide dot blot. Further optimization is needed to minimize the cross-reactivity in assays similar to peptide dot blot. |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rice, Arabidopsis thaliana, Mouse, Rat, Sheep, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Schizosaccharomyces pombe, Corn, Common marmoset | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rice, Arabidopsis thaliana, Mouse, Rat, Sheep, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Schizosaccharomyces pombe, Corn, Common marmoset | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2.00000-4.00000 µg chromatin for 25.00000 µg chromatin | Notes Use AFM ChIP primer pair ab269259 as positive control. |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species Drosophila melanogaster | Dilution info - | Notes - |
Species Arabidopsis thaliana | Dilution info 2.00000-4.00000 µg for 25.00000 µg chromatin | Notes Use AFM ChIP primer pair ab269259 as positive control. |
Species Rice | Dilution info 2.00000-4.00000 µg for 25.00000 µg chromatin | Notes Use AFM ChIP primer pair ab269259 as positive control. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Sheep, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Schizosaccharomyces pombe, Corn, Common marmoset | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.00025-1.00000 µg/mL | Notes when testing with immunogen peptide. |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rice, Arabidopsis thaliana, Mouse, Rat, Sheep, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Schizosaccharomyces pombe, Corn, Common marmoset | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Cow | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Drosophila melanogaster | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rice, Arabidopsis thaliana, Mouse, Rat, Sheep, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Schizosaccharomyces pombe, Corn, Common marmoset | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rice, Arabidopsis thaliana, Mouse, Rat, Sheep, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Schizosaccharomyces pombe, Corn, Common marmoset | Dilution info - | Notes - |
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C3, H3C2, HIST1H3F, H3FI, H3C7, HIST1H3E, H3FD, H3C1, H3FA, HIST1H3A, H3C8, H3FL, HIST1H3B, H3FC, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FH, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3K9me2
Mouse Monoclonal H3 di methyl K9 antibody. Suitable for Dot, ICC/IF, ChIP, ELISA, WB, IHC-P and reacts with Human, Cow, Drosophila melanogaster, Arabidopsis thaliana, Rice samples. Cited in 961 publications.
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C3, H3C2, HIST1H3F, H3FI, H3C7, HIST1H3E, H3FD, H3C1, H3FA, HIST1H3A, H3C8, H3FL, HIST1H3B, H3FC, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FH, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3K9me2
IgG2a
Mouse
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Liquid
Monoclonal
mAbcam 1220
Affinity purification Protein G
By peptide ELISA ab1220 recognizes di methyl K9, but not unmodified K9, mono methyl K9, tri methyl K9, di methyl K27, tri methyl K27, mono methyl K4, di methyl K4 or tri methyl K4. By Western blot ab1220 is blocked by di methyl K9, but not by unmodified K9, mono methyl K9, tri methyl K9, di methyl K27, tri methyl K27, mono methyl K4, di methyl K4 or tri methyl K4. This indicates the specificity of ab1220 for di methyl K9 of Histone H3.
kappa
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Learn about ChIP assay kits, other ChIP antibodies, and more in the ChIP assay guide.
ChIP protocols:
ChIP protocol for cross-linking ChIP (X-ChIP)
Native ChIP protocol
Chromatin preparation from tissues for ChIP
ChIP troubleshooting
ChIP tips and tricks guide
This antibody clone [mAbcam 1220] is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Chen L et al investigates role of Trim 28 (Tripartite motif containing 28) in the epithelial to mesenchymal transition which is implicated in cancer metastasis. ChIP was performed using ab1220. ChIP assay was performed in Trim 28 knockdown A549 cells and control (A). ChIP assay was performed in control and Trim28 expressed in A549 cells (B).
All lanes: Western blot - Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (AB1220) at 2.5 µg/mL
Lane 1: Fruit fly embryo tissue lysate - nuclear at 4.5 µg
Lane 2: Fruit fly embryo tissue lysate - nuclear at 1.5 µg
Lane 3: Fruit fly embryo tissue lysate - nuclear at 0.5 µg
All lanes: HRP-conjugated Goat anti-mouse IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 1min
Alarcon V et al investigates the effects of pargyline due to inhibition of LSD1. Levels of LSD1 protein were reduced using siRNA (siLSD1). Huh7 (Human hepatocellular carcinoma) cells were treated with and without siLSD1 and incubated for 24 hours before transfection of HBV genome (A). Western blot analysis shows the reduction of LSD1 after treatment. Covalent post-translational modifications on Histone H3 was determined by ChIP using ab1220 as one of the specific antibodies (C-G). HBV cccDNA and cytoplasmic levels were determined by qPCR (H).
Blocking buffer: 5% milk
All lanes: Western blot - Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (AB1220) at 1/1000 dilution
All lanes: HEK293 at 30 µg
All lanes: HRP conjugated polyclonal Sheep anti-Mouse IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab1220 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first Kb of the transcribed region.
ChIP was performed using ab1220. Naïve Mouse ES cells were resuspended in PBS and crosslinked with 1% formaldehyde for 5 minutes at room temperature. Crosslinking was quenched with 125m M glycine. A mouse anti IgG (ab18413) was used as a control. ChIP -seq for H3K9me2 in ES cells treated with and without vitamin C (image a). H3K9me2 signal genome-wide was plotted comparing untreated and vitamin C-treated cells (image b). Fold change for average H3K9me2 signal at gene promoters. Most gene promoters display a reduction in H3K9me2 in vitamin C-treated ES cells (image c). ChIP-qPCR for H3K9me2 in ES cells with or without vitamin C at gene promoters. ChIP for IgG was performed as a negative control (image d). Asterisks represent P < 0.05 by t test
All lanes: Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)
Lane 1: Calf thymus histone lysate
Lane 2: Calf thymus histone lysate with Histone H3 peptide - unmodified at 1 μg/ml
Lane 3: Calf thymus histone lysate with Human Histone H3 (mono methyl K9) peptide (ab1771) at 1 μg/ml
Lane 4: Calf thymus histone lysate with Human Histone H3 (di methyl K9) peptide (ab1772) at 1 μg/ml
Lane 5: Calf thymus histone lysate with Human Histone H3 (tri methyl K9) peptide (ab1773) at 1 μg/ml
Lane 6: Calf thymus histone lysate with Histone H3 (di methyl K4) peptide (ab7768) at 1 μg/ml
Lane 7: Calf thymus histone lysate with Human Histone H3 (di methyl K27) peptide (ab1781) at 1 μg/ml
Secondary
Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size: 17 kDa
Exposure time: 1 minute
All lanes: Western blot - Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (AB1220)
All lanes: Calf thymus histone lysate
All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Exposure time: 1min
ab1220 staining human kidney sections by IHC-P using EXPOSE IHC detection kit (ab80436). Formalin fixed paraffin embedded tissue sections were pre-treated using heat mediated antigen retrieval (using a pressure cooker) with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab1220, 5μg/ml, for 1 hour at room temperature. DAB was used as the chromogen and the section was counterstained with haematoxylin and mounted with DPX.
ELISA using ab1220 at varying antibody concentrations.
The purple line indicates binding to the Human Histone H3 (di methyl K9) peptide ab1772. Binding to the following peptides was not seen:
Human Histone H3 (unmodified ) peptide (ab2903),
Human Histone H3 (mono methyl K9) peptide (ab1771),
Human Histone H3 (tri methyl K9) peptide (ab1773),
Human Histone H3 (di methyl K27) peptide (ab1781),
Human Histone H3 (tri methyl K27) peptide (ab1782),
Human Histone H3 (mono methyl K4) peptide (ab1340),
Human Histone H3 (di methyl K4) peptide (ab7768),
Human Histone H3 (tri methyl K4) peptide (ab1342).
This indicates the specificity of ab1220 for di methyl K9 of Histone H3.
Lane 1 : Marker.
All blocking and antibody incubation steps were done with 5% milk in 20mM Tris-HCL, and 0.1% TWEEN-20.
All lanes: Western blot - Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (AB1220) at 1 µg/mL
All lanes: HeLa (Human epitherlial carcinoma cell line) Whole Cell Lysate at 20 µg
All lanes: Goat polyclonal to Mouse IgG-H&L- Pre-Adsorbed (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 5min
ab1220 staining Histone H3 (di methyl K9) in HeLa (Human cervix adenocarcinoma epithelial cell) cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab1220 at 5 µg/ml and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2 µg/ml (shown in red). The secondary antibody (shown in green) was ab150117, Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
This product also gave a positive outcome under the same testing conditions in HeLa cells fixed with 100% methanol (5 mins).
Dot blot analysis of Histone H3 (di methyl K9) with ab1220 at 1/1000 dilution (0.889 μg/mL) followed by Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution.
Lane 1: Human Histone H3 peptide
Lane 2: Human Histone H3 (Mono-Methylation R8) peptide
Lane 3: Human Histone H3 (Di-Methylation R8) peptide
Lane 4: Human Histone H3 (Tri-Methylation R8) peptide
Lane 5: Human Histone H3 (Mono-Methylation K9) peptide
Lane 6: Human Histone H3 (Di-Methylation K9) peptide
Lane 7: Human Histone H3 (Tri-Methylation K9) peptide
Lane 8: Human Histone H3 (Mono-Methylation K4) peptide
Lane 9: Human Histone H3 (Di-Methylation K4) peptide
Lane 10: Human Histone H3 (Tri-Methylation K4) peptide
ab1220 has some cross-reactivity with H3R8me2 in peptide dot blot. Further optimization is needed to minimize the cross-reactivity in assays similar to peptide dot blot
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 180 seconds
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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