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Mouse Monoclonal H3 di methyl K9 antibody. Suitable for ICC/IF, ChIP, ELISA, WB, IHC-P and reacts with Human, Cow, Drosophila melanogaster, Arabidopsis thaliana, Rice samples. Cited in 961 publications.
Alternative names=Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C3, H3C2, HIST1H3F, H3FI, H3C7, HIST1H3E, H3FD, H3C1, H3FA, HIST1H3A, H3C8, H3FL, HIST1H3B, H3FC, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FH, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3K9me2
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | ChIP | ELISA | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
| Arabidopsis thaliana | Predicted | Expected | Predicted | Predicted | Predicted |
| Caenorhabditis elegans | Predicted | Predicted | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted | Predicted | Predicted |
| Common marmoset | Predicted | Predicted | Predicted | Predicted | Predicted |
| Corn | Predicted | Predicted | Predicted | Predicted | Predicted |
| Cow | Expected | Expected | Expected | Tested | Expected |
| Drosophila melanogaster | Expected | Expected | Expected | Tested | Expected |
| Rice | Predicted | Expected | Predicted | Predicted | Predicted |
| Saccharomyces cerevisiae | Predicted | Predicted | Predicted | Predicted | Predicted |
| Schizosaccharomyces pombe | Predicted | Predicted | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted | Predicted | Predicted |
Images
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rice, Arabidopsis thaliana, Mouse, Rat, Sheep, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Schizosaccharomyces pombe, Corn, Common marmoset | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-4.00000 µg chromatin for 25.00000 µg chromatin | Notes Use AFM ChIP primer pair ab269259 as positive control. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Species Drosophila melanogaster | Dilution info - | Notes - |
Species Arabidopsis thaliana | Dilution info 2.00000-4.00000 µg for 25.00000 µg chromatin | Notes Use AFM ChIP primer pair ab269259 as positive control. |
Species Rice | Dilution info 2.00000-4.00000 µg for 25.00000 µg chromatin | Notes Use AFM ChIP primer pair ab269259 as positive control. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Schizosaccharomyces pombe, Corn, Common marmoset | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.00025-1.00000 µg/mL | Notes when testing with immunogen peptide. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rice, Arabidopsis thaliana, Mouse, Rat, Sheep, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Schizosaccharomyces pombe, Corn, Common marmoset | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Cow | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Drosophila melanogaster | Dilution info 1.00000-5.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rice, Arabidopsis thaliana, Mouse, Rat, Sheep, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Schizosaccharomyces pombe, Corn, Common marmoset | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rice, Arabidopsis thaliana, Mouse, Rat, Sheep, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Schizosaccharomyces pombe, Corn, Common marmoset | Dilution info - | Notes - |
Recommended products
Mouse Monoclonal H3 di methyl K9 antibody. Suitable for ICC/IF, ChIP, ELISA, WB, IHC-P and reacts with Human, Cow, Drosophila melanogaster, Arabidopsis thaliana, Rice samples. Cited in 961 publications.
Alternative names=Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C3, H3C2, HIST1H3F, H3FI, H3C7, HIST1H3E, H3FD, H3C1, H3FA, HIST1H3A, H3C8, H3FL, HIST1H3B, H3FC, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FH, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3K9me2
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Clone number
mAbcam 1220
- Purification technique
Affinity purification Protein G
- Specificity
By peptide ELISA ab1220 recognizes di methyl K9, but not unmodified K9, mono methyl K9, tri methyl K9, di methyl K27, tri methyl K27, mono methyl K4, di methyl K4 or tri methyl K4. By Western blot ab1220 is blocked by di methyl K9, but not by unmodified K9, mono methyl K9, tri methyl K9, di methyl K27, tri methyl K27, mono methyl K4, di methyl K4 or tri methyl K4. This indicates the specificity of ab1220 for di methyl K9 of Histone H3.
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Learn about ChIP assay kits, other ChIP antibodies, and more in the ChIP assay guide.
ChIP protocols:
ChIP protocol for cross-linking ChIP (X-ChIP)
Native ChIP protocol
Chromatin preparation from tissues for ChIP
ChIP troubleshooting
ChIP tips and tricks guide
This antibody clone [mAbcam 1220] is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
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- Visit the general protocols
- Visit troubleshooting
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