AB177300

Anti-Histone H3 (mono methyl K122) antibody [EPR17897]

RabMAb
Advanced Validation
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal H3 mono methyl K122 antibody. Suitable for WB, PepArr and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, H3C1, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K122me, H3K122me1, H3K122

5 Images

Supplier Data

Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (AB177300)

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300) at 1/5000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: untreated, asynchronous cells at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: G1/S arrested cells (thymidine treatment) at 10 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: G2/M arrested cells (sequential thymidine and nocodazole treatments) at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (AB177300)

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300) at 1/5000 dilution

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) histone extraction lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

true

Exposure time: 15s

Lab

Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (AB177300)

Blocking and dilution buffer : 5% BSA/TBST.

All lanes:

Anti-ASPHD1 antibody - N-terminal (<a href='/en-us/products/unavailable/asphd1-antibody-n-terminal-ab177330'>ab177330</a>) at 1/5000 dilution

Lane 1:

HeLa cytosolic fraction at 10 µg

Lane 2:

HeLa nuclear fraction at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (AB177300)

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300) at 1/5000 dilution

All lanes:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

true

Exposure time: 30s

Peptide Array - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (AB177300)

PepArr

Unknown

Peptide Array - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (AB177300)

ab177300 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.

Carrier free

Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17897

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

PepArr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 mono methyl K122

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:113468 PubMed37995178

2023

BACH1 regulates the differentiation of vascular smooth muscle cells from human embryonic stem cells via CARM1-mediated methylation of H3R17.

Applications

Unspecified application

Species

Unspecified reactive species

Yunquan He,Jieyu Guo,Yueyang Yu,Jiayu Jin,Qingjun Jiang,Qinhan Li,Siyu Ma,Qi Pan,Jiayi Lin,Nan Jiang,Jinghua Ma,Yongbo Li,Yannan Hou,Xiuling Zhi,Lindi Jiang,Lefeng Qu,Elena Osto,Xinhong Wang,Xiangxiang Wei,Dan Meng

View all publications

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peptideArrayWebsite

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (mono methyl K122) antibody [EPR17897]

Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (AB177300)

Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (AB177300)

Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (AB177300)

Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (AB177300)

Peptide Array - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (AB177300)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise