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Rabbit Recombinant Monoclonal H3 mono methyl K122 antibody. Carrier free. Suitable for WB, PepArr and reacts with Human, Mouse, Synthetic peptide samples.

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Images

Peptide Array - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (AB249956), expandable thumbnail
  • Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (AB249956), expandable thumbnail
  • Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (AB249956), expandable thumbnail
  • Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (AB249956), expandable thumbnail
  • Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (AB249956), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBPepArr
Human
Tested
Expected
Mouse
Tested
Expected
Synthetic peptide
Not recommended
Tested

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, H3C1, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K122me

Recommended products

Rabbit Recombinant Monoclonal H3 mono methyl K122 antibody. Carrier free. Suitable for WB, PepArr and reacts with Human, Mouse, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR17897
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab249956 is the carrier-free version of Anti-Histone H3 (mono methyl K122) antibody [EPR17897] ab177300.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Peptide Array - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956), expandable thumbnail

    Peptide Array - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)

    This data was developed using Anti-Histone H3 (mono methyl K122) antibody [EPR17897] ab177300, the same antibody clone in a different buffer formulation.

    Anti-Histone H3 (mono methyl K122) antibody [EPR17897] ab177300 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.

  • Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956), expandable thumbnail

    Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)

    This data was developed using Anti-Histone H3 (mono methyl K122) antibody [EPR17897] ab177300, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% BSA/TBST.

    All lanes: Anti-ASPHD1 antibody - N-terminal (ab177330) at 1/5000 dilution

    Lane 1: HeLa cytosolic fraction at 10 µg

    Lane 2: HeLa nuclear fraction at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

    Exposure time: 30s

  • Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956), expandable thumbnail

    Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)

    This data was developed using Anti-Histone H3 (mono methyl K122) antibody [EPR17897] ab177300, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% BSA/TBST.

    All lanes: Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (Anti-Histone H3 (mono methyl K122) antibody [EPR17897] ab177300) at 1/5000 dilution

    All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) histone extraction lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

    Exposure time: 15s

  • Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956), expandable thumbnail

    Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)

    This data was developed using Anti-Histone H3 (mono methyl K122) antibody [EPR17897] ab177300, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% BSA/TBST.

    All lanes: Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956) at 1/5000 dilution

    All lanes: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

    Exposure time: 30s

  • Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956), expandable thumbnail

    Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)

    This data was developed using Anti-Histone H3 (mono methyl K122) antibody [EPR17897] ab177300, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% BSA/TBST.

    All lanes: Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (Anti-Histone H3 (mono methyl K122) antibody [EPR17897] ab177300) at 1/5000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: untreated, asynchronous cells at 10 µg

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: G1/S arrested cells (thymidine treatment) at 10 µg

    Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: G2/M arrested cells (sequential thymidine and nocodazole treatments) at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

    Exposure time: 30s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com