AB194688

Anti-Histone H3 (mono methyl K27) antibody

Name: Anti-Histone H3 (mono methyl K27) antibody (ab194688) | Abcam
Brand: Abcam Limited
SKU: AB194688
Price: 535 USD
Availability: InStock
Rating: 2 (1 reviews)

(1 Review)

(12 Publications)

Rabbit Polyclonal H3 mono methyl K27 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Mouse samples. Cited in 12 publications.

View Alternative Names

H3FT, HIST3H3, H3-4, Histone H3.1t, H3/t, H3t, H3/g, Histone H3.4, H3K27me, H3K27me1, H3K27

2 Images

Supplier Data

Western blot - Anti-Histone H3 (mono methyl K27) antibody (AB194688)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-Histone H3 (mono methyl K27) antibody (ab194688)

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

H3 protein at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 15 kDa

false

Supplier Data

Dot Blot - Anti-Histone H3 (mono methyl K27) antibody (AB194688)

Dot-blot analysis of methylation peptides using ab194688.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3-4 mono methyl K27

Additional targets

,H3C1 mono methyl K27

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:553 PubMed40701963

2025

H3K14 lactylation exacerbates neuronal ferroptosis by inhibiting calcium efflux following intracerebral hemorrhagic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Sun,Jian-Nan Zhang,Ting Lan,Lei Shi,Liye Hu,Liyan Yan,Chao Wei,Lisha Hei,Weihua Wu,Zhaoli Luo,Meng Liu,Xingmei Ren,Yamei Wang,Yabin Lu,Peipei Wang,Chenguang Zhang,Liheng Bian,Xingquan Zhao,Fei Yang,Qian Li

Cell reports 44:115213 PubMed39817906

2025

Coordinated neuron-specific splicing events restrict nucleosome engagement of the LSD1 histone demethylase complex.

Applications

Unspecified application

Species

Unspecified reactive species

Robert S Porter,Sojin An,Maria C Gavilan,Masayoshi Nagai,Yumie Murata-Nakamura,Bo Zhou,Katherine M Bonefas,Olivier Dionne,Jeru Manoj Manuel,Joannie St-Germain,Suzanne Gascon,Jacqueline Kim,Liam Browning,Benoit Laurent,Uhn-Soo Cho,Shigeki Iwase

Life (Basel, Switzerland) 14: PubMed39768289

2025

Enrichment of H3S28p and H3K9me2 Epigenetic Marks on Inflammatory-Associated Gene Promoters in Response to Severe Burn Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Osvaldo Arias-Pérez,Thelma Escobedo-Tapia,Cecilia Cintora-Ahumada,Lizbel León-Solís,Norberto Leyva-García,Elena Aréchaga-Ocampo,Rafael Franco-Cendejas,Oscar Hernández-Hernández,Rocío Suárez-Sánchez

Nature communications 15:5470 PubMed38937441

2024

Temperature-dependent jumonji demethylase modulates flowering time by targeting H3K36me2/3 in Brassica rapa.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyun Xin,Peirong Li,Xiuyun Zhao,Yangjun Yu,Weihong Wang,Guihua Jin,Jiao Wang,Liling Sun,Deshuang Zhang,Fenglan Zhang,Shuancang Yu,Tongbing Su

Clinical epigenetics 16:51 PubMed38576048

2024

Targeting histone demethylases JMJD3 and UTX: selenium as a potential therapeutic agent for cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Chen,Bo Cai,Yingying Zhu,Yimin Ma,Xiaoting Yu,Jieqi Xiong,Jiaying Shen,Weiwei Tie,Yisheng Zhang,Fei Guo

Clinical science (London, England : 1979) 138:251-268 PubMed38362910

2024

Inhibition of the histone methyltransferase EZH2 induces vascular stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Jaime Ibarrola,Rachel R Xiang,Zhe Sun,Qing Lu,Michael A Hill,Iris Z Jaffe

Cell death and differentiation 31:106-118 PubMed38012390

2023

H3K36 methyltransferase NSD1 protects against osteoarthritis through regulating chondrocyte differentiation and cartilage homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Shao,Jinlong Suo,Zhong Zhang,Mingxiang Kong,Yiyang Ma,Yang Wen,Mengxue Liu,Lenan Zhuang,Kai Ge,Qing Bi,Changqing Zhang,Weiguo Zou

eLife 11: PubMed36125117

2022

H3K9me1/2 methylation limits the lifespan of mutants in .

Applications

Unspecified application

Species

Unspecified reactive species

Meng Huang,Minjie Hong,Xinhao Hou,Chengming Zhu,Di Chen,Xiangyang Chen,Shouhong Guang,Xuezhu Feng

Science (New York, N.Y.) 374:eabi7489 PubMed34762468

2021

The genetic and epigenetic landscape of the centromeres.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Naish,Michael Alonge,Piotr Wlodzimierz,Andrew J Tock,Bradley W Abramson,Anna Schmücker,Terezie Mandáková,Bhagyshree Jamge,Christophe Lambing,Pallas Kuo,Natasha Yelina,Nolan Hartwick,Kelly Colt,Lisa M Smith,Jurriaan Ton,Tetsuji Kakutani,Robert A Martienssen,Korbinian Schneeberger,Martin A Lysak,Frédéric Berger,Alexandros Bousios,Todd P Michael,Michael C Schatz,Ian R Henderson

Journal of experimental & clinical cancer research 39:66 PubMed32293500

2020

PERK/NRF2 and autophagy form a resistance mechanism against G9a inhibition in leukemia stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Eun Jang,Ju-In Eom,Hoi-Kyung Jeung,Haerim Chung,Yu Ri Kim,Jin Seok Kim,June-Won Cheong,Yoo Hong Min

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (mono methyl K27) antibody

Western blot - Anti-Histone H3 (mono methyl K27) antibody (AB194688)

Dot Blot - Anti-Histone H3 (mono methyl K27) antibody (AB194688)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (12)

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