AB195492

Anti-Histone H3 (mono methyl K27) antibody

Name: Anti-Histone H3 (mono methyl K27) antibody (ab195492) | Abcam
Brand: Abcam Limited
SKU: AB195492
Price: 515 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal H3 mono methyl K27 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Synthetic peptide samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H3C1 mono methyl K27 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, HIST1H3C, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27me, H3K27me1, H3K27

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K27) antibody (AB195492)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K27) antibody (AB195492)

Immunofluorescent analysis of Human osteosarcoma (U2OS) cells labeling Histone H3 (mono methyl K27) with ab195492 at 1/1000 dilution in blocking solution followed by A (top row) an anti-rabbit antibody conjugated to Alexa568 (left) or with DAPI (right), which specifically labels DNA. Cells were fixed with 4% formaldehyde for 20 minutes and blocked with PBS/TX-100 containing 5% normal goat serum.

Rows 2-5 : staining of the cells with the ab195492 after incubation of the antibody with 2 ng/μl blocking peptide containing the unmodified (row 2) and the mono- (row 3), di- (row 4) and trimethylated H3K27 (row 5), respectively.

ChIP - Anti-Histone H3 (mono methyl K27) antibody (AB195492)

ChIP

Supplier Data

ChIP - Anti-Histone H3 (mono methyl K27) antibody (AB195492)

ChIP analyis using Human HeLa cells, labeling Histone H3 (mono methyl K27) with ab195492 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the active gene GAPDH used as a negative and a positive control target, respectively. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Supplier Data

Western blot - Anti-Histone H3 (mono methyl K27) antibody (AB195492)

All lanes:

Western blot - Anti-Histone H3 (mono methyl K27) antibody (ab195492) at 1/1000 dilution

All lanes:

Histone extracts from HeLa cells at 15 µg

Predicted band size: 15 kDa

false

Supplier Data

Dot Blot - Anti-Histone H3 (mono methyl K27) antibody (AB195492)

Dot Blot analysis of peptides containing modified and unmodified sequences of histone H3 and H4, labeling Histone H3 (mono methyl K27) with ab195492 at 1/20000 dilution. 0.2-100 pmol of the peptide containing the respective histone modification were spotted onto the membrane.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Dot, ChIP, ICC/IF

applications

Immunogen

Synthetic Peptide within Human H3C1 mono methyl K27 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P68431

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "1 μg/ChIP.", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Arabidopsis thaliana": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.05% Sodium azide, 0.05% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 mono methyl K27

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:5470 PubMed38937441

2024

Temperature-dependent jumonji demethylase modulates flowering time by targeting H3K36me2/3 in Brassica rapa.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyun Xin,Peirong Li,Xiuyun Zhao,Yangjun Yu,Weihong Wang,Guihua Jin,Jiao Wang,Liling Sun,Deshuang Zhang,Fenglan Zhang,Shuancang Yu,Tongbing Su

Genome biology 21:104 PubMed32349780

2020

Wheat chromatin architecture is organized in genome territories and transcription factories.

Applications

Unspecified application

Species

Unspecified reactive species

Lorenzo Concia,Alaguraj Veluchamy,Juan S Ramirez-Prado,Azahara Martin-Ramirez,Ying Huang,Magali Perez,Severine Domenichini,Natalia Y Rodriguez Granados,Soonkap Kim,Thomas Blein,Susan Duncan,Clement Pichot,Deborah Manza-Mianza,Caroline Juery,Etienne Paux,Graham Moore,Heribert Hirt,Catherine Bergounioux,Martin Crespi,Magdy M Mahfouz,Abdelhafid Bendahmane,Chang Liu,Anthony Hall,Cécile Raynaud,David Latrasse,Moussa Benhamed

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (mono methyl K27) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K27) antibody (AB195492)

ChIP - Anti-Histone H3 (mono methyl K27) antibody (AB195492)

Western blot - Anti-Histone H3 (mono methyl K27) antibody (AB195492)

Dot Blot - Anti-Histone H3 (mono methyl K27) antibody (AB195492)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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