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AB272157

Anti-Histone H3 (mono methyl K36) antibody

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Rabbit Polyclonal H3 mono methyl K36 antibody. Suitable for Dot, WB, ICC/IF and reacts with Synthetic peptide, Caenorhabditis elegans, Human samples. Immunogen corresponding to Synthetic Peptide within Human H3C1 mono methyl K36.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K36me, H3K36me1, H3K36

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K36) antibody (AB272157)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K36) antibody (AB272157)

Immunofluorescence analysis of 0.5% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (mono methyl L36) with ab272157 at 1/100 dilution for 1 hour at room temperature, followed by Dylight 488 secondary antibody at 1/10000 for 45 minutes at room temperature. Staining of Histone H3 (mono methyl L36) is green. Nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).

Western blot - Anti-Histone H3 (mono methyl K36) antibody (AB272157)
  • WB

Unknown

Western blot - Anti-Histone H3 (mono methyl K36) antibody (AB272157)

All lanes:

Western blot - Anti-Histone H3 (mono methyl K36) antibody (ab272157) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) histone preps at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Western blot - Anti-Histone H3 (mono methyl K36) antibody (AB272157)
  • WB

Unknown

Western blot - Anti-Histone H3 (mono methyl K36) antibody (AB272157)

All lanes:

Western blot - Anti-Histone H3 (mono methyl K36) antibody (ab272157) at 1/1000 dilution

All lanes:

Caenorhabditis elegans embryo lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Dot Blot - Anti-Histone H3 (mono methyl K36) antibody (AB272157)
  • Dot

Unknown

Dot Blot - Anti-Histone H3 (mono methyl K36) antibody (AB272157)

Dot Blot of ab272157.

Lane 1 : K36.

Lane 2 : K36Me1.

Lane 3 : K36Me2.

Lane 4 : K36Me3.

Lane 5 : K36ac.

Load : 0.3, 0.6, 1, 3, 6, and 10 picomoles of peptide. Primary antibody ab272157 at 0.5 μg/ml for 45 minutes at 4°C. Dylight™488 rabbit secondary antibody at 1/10000 for 45 minutes at room temperature.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Caenorhabditis elegans

Applications

Dot, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human H3C1 mono methyl K36. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Rat": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Anti-Histone H3 [Monomethyl Lys36] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 mono methyl K36
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Product promise

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