Rabbit Polyclonal H3 mono methyl K36 antibody. Suitable for ICC/IF, ChIP, WB and reacts with Human samples. Cited in 112 publications.
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- Life science alliance 6:2023Chromatin in 3D distinguishes dMes-4/NSD and Hypb/dSet2 in protecting genes from H3K27me3 silencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Depierre,Charlène Perrois,Naomi Schickele,Priscillia Lhoumaud,Mahdia Abdi-Galab,Olivier Fosseprez,Alexandre Heurteau,Raphaël Margueron,Olivier CuvierPubMed 37684044
- Journal of cardiovascular development and disease 10:2023H3K36 Di-Methylation Marks, Mediated by Ash1 in Complex with Caf1-55 and MRG15, Are Required during Heart Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Yi Zhu,Chen Liu,Xiaohu Huang,Joyce van de Leemput,Hangnoh Lee,Zhe HanPubMed 37504562
- International journal of molecular sciences 24:2023The Arabidopsis Deubiquitylase OTU5 Suppresses Flowering by Histone Modification-Mediated Activation of the Major Flowering Repressors , , and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesRamalingam Radjacommare,Shih-Yun Lin,Raju Usharani,Wen-Dar Lin,Guang-Yuh Jauh,Wolfgang Schmidt,Hongyong FuPubMed 37047144
- Pathogens (Basel, Switzerland) 11:2022[PRION] States Are Associated with Specific Histone H3 Post-Translational Modification Changes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamantha N Cobos,Chaim Janani,Gabriel Cruz,Navin Rana,Elizaveta Son,Rania Frederic,Jailene Paredes Casado,Maliha Khan,Seth A Bennett,Mariana P TorrentePubMed 36558770
- Frontiers in cell and developmental biology 10:9456682022Elevated levels of the methyltransferase SETD2 causes transcription and alternative splicing changes resulting in oncogenic phenotypes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaikat Bhattacharya,Divya Reddy,Ning Zhang,Hua Li,Jerry L WorkmanPubMed 36035998
- Acta pharmaceutica Sinica. B 12:1871-18842022Nuclear TIGAR mediates an epigenetic and metabolic autoregulatory loop NRF2 in cancer therapeutic resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Wang,Qianqian Wang,Guodi Cai,Zhijian Duan,Zoann Nugent,Jie Huang,Jianwei Zheng,Alexander D Borowsky,Jian Jian Li,Peiqing Liu,Hsing-Jien Kung,Leigh Murphy,Hong-Wu Chen,Junjian WangPubMed 35847493
- American journal of physiology. Renal physiology 323:F227-F2422022Cross talk between lysine methyltransferase Smyd2 and TGF-β-Smad3 signaling promotes renal fibrosis in autosomal dominant polycystic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinda Xiaoyan Li,Lu Zhang,Ewud Agborbesong,Xiaoqin Zhang,Julie Xia Zhou,Xiaogang LiPubMed 35759739
- International journal of biological sciences 18:3621-36352022NUF2 Drives Clear Cell Renal Cell Carcinoma by Activating HMGA2 Transcription through KDM2A-mediated H3K36me2 Demethylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiatian Lin,Xiangling Chen,Hongjian Yu,Shasha Min,Yequn Chen,Zesong Li,Xina XiePubMed 35813477
- Molecular cell 82:816-832.e122022SETDB1/NSD-dependent H3K9me3/H3K36me3 dual heterochromatin maintains gene expression profiles by bookmarking poised enhancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmandine Barral,Gabrielle Pozo,Lucas Ducrot,Giorgio L Papadopoulos,Sandrine Sauzet,Andrew J Oldfield,Giacomo Cavalli,Jérôme DéjardinPubMed 35081363
- Bone research 9:302021H3K36 methyltransferase NSD1 regulates chondrocyte differentiation for skeletal development and fracture repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Shao,Zhong Zhang,Zhan Xu,Huiling Ouyang,Lijun Wang,Hongwei Ouyang,Matthew Greenblatt,Xi Chen,Weiguo ZouPubMed 34099628
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | ChIP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Arabidopsis thaliana | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Drosophila melanogaster | Predicted | Predicted | Predicted |
| Mammals | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Saccharomyces cerevisiae | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Pig, Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster, Mammals | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg chromatin for 25 µg chromatin | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Pig, Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster, Mammals | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Pig, Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster, Mammals | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C7, H3C1, H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3K36me
Recommended products
Rabbit Polyclonal H3 mono methyl K36 antibody. Suitable for ICC/IF, ChIP, WB and reacts with Human samples. Cited in 112 publications.
Alternative names
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C7, H3C1, H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3K36me
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
Specific for human Histone H3 mono methyl K36. Shows partial cross-reactivity with di-methyl K36 (please see Western Blot image).
This antibody may not be suitable for experiments on yeast lysate. Although the antibody is specifically blocked using the immunising peptide, customer feedback indicates that it detects a band using S. cerevisiae K36 point mutants. We welcome further customer feedback.- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Image courtesy of an anonymous customer review.Western blot - Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048)
All lanes: Western blot - Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048) at 1/1400 dilution
All lanes: Whole cell lysate prepared from Drosophila BG3 cells at 500000 Cells
SecondaryAll lanes: HRP donkey anti-rabbit monoclonal at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 15 kDa
Observed band size: 17 kDa, 42 kDa
Exposure time: 30s
ChIP - Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048)
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab9048 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
Western blot - Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048)
Secondary ab: Alexa Fluor 680 Goat anti-rabbit IgG
0.5μg histone prep used per lanePrimary antibody:
Lane 1: ab9048 (Histone H3 Mono Methyl K36) 1/500
Lane 2: ab9048 (Histone H3 Mono Methyl K36) 1/500 + ab1783 (ab9048) (Histone H3 Mono Methyl K36) peptide 1μg/ml
Lane 3: ab9048 (Histone H3 Mono Methyl K36) 1/500 + ab1794 (Anti-Histone H3 (di methyl K36) antibody - ChIP Grade ab9049) (Histone H3 Di Methyl K36) peptide 1μg/ml
Lane 4: ab9048 (Histone H3 Mono Methyl K36) 1/500 + Human Histone H3 (tri methyl K36) peptide ab1785 (Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade ab9050) (Histone H3 Tri Methyl K36) peptide 1μg/ml
Lane 5: ab9048 (Histone H3 Mono Methyl K36) 1/500 + Human Histone H3 (unmodified ) peptide ab2623 (Histone H3 (23-34) – unmodified) peptide 1μg/ml
Lane 6: ab9048 (Histone H3 Mono Methyl K36) 1/500 + Human Histone H3 (mono methyl K4) peptide ab1340 (Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade ab8895) (Histone H3 Mono methyl K4) peptide 1μg/mlab9048 specifically recognise
All lanes: Western blot - Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048) at 1/500 dilution
All lanes: Histone prep at 0.5 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution
Predicted band size: 15 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048)
ab9048 staining Histone H3 (mono methyl K36) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab9048 at 0.1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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