AB176920

Anti-Histone H3 (mono methyl K36) antibody [EPR16993]

RabMAb
Advanced Validation
Recombinant
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(9 Publications)

Rabbit Recombinant Monoclonal H3 mono methyl K36 antibody. Suitable for WB, PepArr, ICC/IF and reacts with Mouse, Human samples. Cited in 9 publications.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K36me, H3K36me1, H3K36

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] (AB176920)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] (AB176920)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H3 (mono methyl K36) with ab176920 at 1/2000 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab176920 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Supplier Data

Western blot - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] (AB176920)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] (ab176920) at 1/10000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

Lane 2:

NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 5s

Peptide Array - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] (AB176920)

PepArr

Unknown

Peptide Array - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] (AB176920)

ab176920 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.

Carrier free

Anti-Histone H3 (mono methyl K36) antibody [EPR16993] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16993

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

PepArr, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 mono methyl K36

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8898 PubMed41057304

2025

Fate mapping of peripherally-derived macrophages after traumatic brain injury in mice reveals a long-lasting population with a distinct transcriptomic signature.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Serena Paladini,Benjamin A Yang,Kristof A Torkenczy,Elma S Frias,Xi Feng,Karen Krukowski,Rene Sit,Maurizio Morri,Wendy Lam,Valentina Pedoia,Stefka Tyanova,Marco Colonna,Amber L Nolan,Susanna Rosi

Nature communications 15:5470 PubMed38937441

2024

Temperature-dependent jumonji demethylase modulates flowering time by targeting H3K36me2/3 in Brassica rapa.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyun Xin,Peirong Li,Xiuyun Zhao,Yangjun Yu,Weihong Wang,Guihua Jin,Jiao Wang,Liling Sun,Deshuang Zhang,Fenglan Zhang,Shuancang Yu,Tongbing Su

Reproduction (Cambridge, England) 167: PubMed38236723

2024

Decreased NSD2 impairs stromal cell proliferation in human endometrium via reprogramming H3K36me2.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan-Mei Qin,Xiao-Wei Wei,Jia-Yi Wu,Xue-Qing Liu,Yi Lin

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2202737 PubMed36941223

2023

The PRMT6/PARP1/CRL4B Complex Regulates the Circadian Clock and Promotes Breast Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tianshu Yang,Wei Huang,Tianyu Ma,Xin Yin,Jingyao Zhang,Miaomiao Huo,Ting Hu,Tianyang Gao,Wei Liu,Die Zhang,Hefen Yu,Xu Teng,Min Zhang,Hao Qin,Yunkai Yang,Baowen Yuan,Yan Wang

International journal of molecular sciences 23: PubMed36233308

2022

Oocyte-Specific Knockout of Histone Lysine Demethylase KDM2a Compromises Fertility by Blocking the Development of Follicles and Oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xianrong Xiong,Xiaojian Zhang,Manzhen Yang,Yanjin Zhu,Hailing Yu,Xixi Fei,Fuko Mastuda,Daoliang Lan,Yan Xiong,Wei Fu,Shi Yin,Jian Li

Horticulture research : PubMed35043207

2022

Histone H3K27 demethylase SlJMJ4 promotes dark- and ABA- induced leaf senescence in tomato.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochun Ding,Dandan Zhang,Dachuan Gu,Zhiwei Li,Hanzhi Liang,Hong Zhu,Yueming Jiang,Xuewu Duan

Cell 184:3915-3935.e21 PubMed34174187

2021

The single-cell epigenomic and transcriptional landscape of immunity to influenza vaccination.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Wimmers,Michele Donato,Alex Kuo,Tal Ashuach,Shakti Gupta,Chunfeng Li,Mai Dvorak,Mariko Hinton Foecke,Sarah E Chang,Thomas Hagan,Sanne E De Jong,Holden T Maecker,Robbert van der Most,Peggie Cheung,Mario Cortese,Steven E Bosinger,Mark Davis,Nadine Rouphael,Shankar Subramaniam,Nir Yosef,Paul J Utz,Purvesh Khatri,Bali Pulendran

Genes & development 33:103-115 PubMed30578303

2018

Timely double-strand break repair and pathway choice in pericentromeric heterochromatin depend on the histone demethylase dKDM4A.

Applications

Unspecified application

Species

Unspecified reactive species

Aniek Janssen,Serafin U Colmenares,Timothy Lee,Gary H Karpen

Cell 173:1385-1397.e14 PubMed29706550

2018

Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Peggie Cheung,Francesco Vallania,Hayley C Warsinske,Michele Donato,Steven Schaffert,Sarah E Chang,Mai Dvorak,Cornelia L Dekker,Mark M Davis,Paul J Utz,Purvesh Khatri,Alex J Kuo

View all publications

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peptideArrayWebsite

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (mono methyl K36) antibody [EPR16993]

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] (AB176920)

Western blot - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] (AB176920)

Peptide Array - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] (AB176920)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (9)

Product promise