AB272160

Anti-Histone H3 (mono methyl K37) antibody

Name: Anti-Histone H3 (mono methyl K37) antibody (ab272160) | Abcam
Brand: Abcam Limited
SKU: AB272160
Price: 640 USD
Availability: InStock

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Rabbit Polyclonal H3 mono methyl K37 antibody. Suitable for Dot, WB, IF and reacts with Synthetic peptide, Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human H3C1 mono methyl K37.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K37me, H3K37me1, H3K37

4 Images

Supplier Data

Immunofluorescence - Anti-Histone H3 (mono methyl K37) antibody (AB272160)

Immunofluorescence analysis of Anti-Histone H3K37me. ab272160 was tested at 1 : 200 in HeLa cells with FITC (green). Cells and nuclei were counterstained with DAPI (blue) and DyLight 550 (red). (40X)

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Western blot - Anti-Histone H3 (mono methyl K37) antibody (AB272160)

Block : 5% BLOTTO overnight at 4°C.

All lanes:

Western blot - Anti-Histone H3 (mono methyl K37) antibody (ab272160) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 30 µg

Lane 2:

NIH/3T3 cell lysate at 30 µg

Lane 3:

Elegans embryo lysate at 30 µg

Secondary

All lanes:

RDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT at 1/10000 dilution

Predicted band size: 15 kDa

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Dot Blot - Anti-Histone H3 (mono methyl K37) antibody (AB272160)

Dot Blot analysis of ab272160.

Lane 1 : K37me1.

Lane 2 : K36me1.

Lane 3 : K36me2.

Lane 4 : K36me3.

Lane 5 : K36ac.

Load : 1, 10, and 100 picomoles of peptide. Primary antibody : ab272160 at 1 : 1000 for 45 minutes at 4°C. Secondary antibody : Dylight™488 rabbit secondary antibody at 1 : 10,000 for 45 minutes at RT. Block : 5% BLOTTO overnight at 4°C.

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Dot Blot - Anti-Histone H3 (mono methyl K37) antibody (AB272160)

Dot Blot analysis of ab272160.

Lane 1 : K37.

Lane 2 : K37Ac.

Lane 3 : K37Me1.

Lane 4 : K37Me2.

Lane 5 : K37Me3.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IF, Dot, WB

applications

Immunogen

Synthetic Peptide within Human H3C1 mono methyl K37. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IF" : {"fullname" : "Immunofluorescence", "shortname":"IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IF-species-checked": "testedAndGuaranteed", "IF-species-dilution-info": "1/200", "IF-species-notes": "" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IF-species-checked": "guaranteed", "IF-species-dilution-info": "", "IF-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IF-species-checked": "notRecommended", "IF-species-dilution-info": "", "IF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Anti-Histone H3 [Monomethyl Lys37] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 mono methyl K37

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (mono methyl K37) antibody

Immunofluorescence - Anti-Histone H3 (mono methyl K37) antibody (AB272160)

Western blot - Anti-Histone H3 (mono methyl K37) antibody (AB272160)

Dot Blot - Anti-Histone H3 (mono methyl K37) antibody (AB272160)

Dot Blot - Anti-Histone H3 (mono methyl K37) antibody (AB272160)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise