AB8895

Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade

Name: Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) | Abcam
Brand: Abcam Limited
SKU: AB8895
Price: 450 USD
Availability: InStock
Rating: 4 (58 reviews)

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What is this?

(58 Reviews)

(1277 Publications)

Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ChIP. Suitable for Cow, Human, Mouse, Rat.

- Over 1120 publications
- Trusted since 2002

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me, H3K4me1, H3K4

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

IHC image of ab8895 staining Histone H3 (mono methyl K4) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8895, 0.5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ChIP - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

ChIP

AbReview65555****

ChIP - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

ChIP analysis using ab8895 binding Histone H3 (mono methyl K4) in Metastatic clear cell renal carcinoma cells (M1A). Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated at 1/100 dilution with primary antibody for 12 hours at 4°C.
Positive control : Metastatic clear cell renal carcinoma cells (M1A).
Negative Control : Parental clear cell renal carcinoma cells (786-O).

This image is courtesy of an Abreview submitted by Paulo Rodrigues

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

ab8895 staining Histone H3 (mono methyl K4) in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab8895 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

ChIP

Unknown

ChIP - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

Chromatin was prepared from U-2 OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab8895 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH and ALDOA (active) and MYO-D (inactive) promoters and over the y-Actin gene (active). Schematic diagram of the y-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.

Lab

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

Blocking buffer : 2% BSA

Gel type : MES

All lanes:

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) at 1 µg/mL

Lane 1:

Calf Thymus Histone at 0.5 µg

Lane 2:

HeLa Nuclear – Triton Prep at 10 µg

Lane 3:

NIH3T3 Nuclear – Triton Prep at 10 µg

Lane 4:

PC12 Nuclear at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 30s

Unknown

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

ab8895 is specific for mono-methylated Lysine 4 of histone H3 and does not recognize di- or tri-methyl Lysine 4 nor methylation at Lysine 9. This is shown in lane 2 where the activity of the antibody is specifically blocked by the addition of the immunizing peptide (ab1340).

All lanes:

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) at 1/500 dilution

All lanes:

Calf thymus histone lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 18 kDa

false

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Cow, Human, Mouse, Rat

Applications

ChIP, WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Specific for mono-methylated Lysine 4 of histone H3. Does not recognise di- or tri-methyl Lysine 4 nor methylation at Lysine 9.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Works better if cells fixed in methanol.", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2 µg for 25 µg chromatin", "ChIP-species-notes": "We recommend Myo-D ChIP primer pair <a href='/en-us/products/reagents/myo-d-positive-control-chip-primer-pair-ab269261'>ab269261</a> as positive control.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1 - 1/5", "WB-species-notes": "" }, "Candida albicans": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mammals": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Plants": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Plasmodium falciparum": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Saccharomyces cerevisiae": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Tetrahymena": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) is a rabbit polyclonal antibody and is validated for use in ChIP, ICC/IF, IHC-P and WB.

Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) was first used in a scientific publication in 2003 and has been cited over 1122 times in peer reviewed journals. It's performance in ChIP in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) has high sensitivity and specificity.

Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) has 58 independent reviews from customers.

Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) specifically detects Histone H3 Mono Methyl-K4 (UniProt ID: P68431; Molecular weight: 16kDa) and is sold in 50 µg selling sizes.

Top cited antibody for this target with >1000 citations. A highly cited Histone H3 mono methyl K4 antibody, essential for studying epigenetic modifications and gene activation. This antibody is crucial in chromatin structure research, particularly in understanding the role of H3K4me1 in transcription regulation. It is widely used in as a marker for histone methylation and enhancer regions.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 mono methyl K4

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Publications (1277)

Recent publications for all applications. Explore the full list and refine your search

Scientific data 12:1620 PubMed41057362

2025

Epigenomics and transcriptomics profiles of developing zebrafish heart cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gulrez Chahal,Michael P Eichenlaub,Markus Tondl,Michał Pawlak,Monika Mohenska,Lin Grimm,Lauren Bottrell,Mark Drvodelic,Sara Alaei,Jeannette Hallab,Lisa N Waylen,Jose M Polo,Cédric Blanpain,Nathan Palpant,Fernando J Rossello,Minna-Liisa Änkö,Peter D Currie,Benjamin M Hogan,Cecilia Winata,Ekaterina Salimova,Hieu T Nim,Mirana Ramialison

iScience 28:113426 PubMed41054521

2025

Ablation of histone H1.4 reveals the focal regulation of AP-1 directed enhancers.

Applications

Unspecified application

Species

Unspecified reactive species

Nolan G Gokey,James M Ward,Eric J Milliman,Leesa J Deterding,Kevin W Trotter,Trevor K Archer

Nature genetics 57:2468-2481 PubMed41044247

2025

Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Luo,Zhixiang Chen,Yuanyuan Qiao,Jean Ching-Yi Tien,Eleanor Young,Rahul Mannan,Somnath Mahapatra,Rupam Bhattacharyya,Lanbo Xiao,Tongchen He,Sanjana Eyunni,Yuping Zhang,Yang Zheng,Fengyun Su,Xuhong Cao,Rui Wang,Yunhui Cheng,Rithvik Seri,James George,Miriam Shahine,Stephanie J Miner,Matthew G Rees,Melissa M Ronan,Jennifer A Roth,Ulka Vaishampayan,Mi Wang,Shaomeng Wang,Abhijit Parolia,Arul M Chinnaiyan

PLoS genetics 21:e1011608 PubMed40971994

2025

Epigenome and transcriptome changes in KMT2D-related Kabuki syndrome Type 1 iPSCs, neuronal progenitors and cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Cuvertino,Evgenii Martirosian,Kedar Bhosale,Peiwen Cheng,Terence Garner,Ian J Donaldson,Adam Jackson,Adam Stevens,Andrew D Sharrocks,Susan J Kimber,Siddharth Banka

JCI insight 10: PubMed40956618

2025

In utero rescue of neurological dysfunction in a mouse model of Wiedemann-Steiner syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Tinna Reynisdottir,Kimberley J Anderson,Katrin Möller,Stefán Pétursson,Andrew Brinn,Katheryn P Franklin,Juan Ouyang,Asbjorg O Snorradottir,Cathleen M Lutz,Aamir R Zuberi,Valerie B DeLeon,Hans T Bjornsson

Poultry science 104:105746 PubMed40896951

2025

Establishing the super-enhancer landscape in chicken adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Mu,Yang Jing,Jiarui He,Junjie Hou,Jiaxin Huang,Jiayi Zhang,Fan Gao,Ming Lou,Xiaohong Yan,Ning Wang

Nature genetics 57:2264-2275 PubMed40890361

2025

Chromatin dynamics of a large-sized genome provides insights into polyphenism and X0 dosage compensation of locusts.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Liu,Feng Jiang,Ran Li,Shanlin Liu,Wenjing Fang,Xiao Li,Ting Hu,Lianyun Feng,Jie Zhang,Zhikang Liu,Jing He,Wei Guo,Xianhui Wang,Zongyi Sun,Jingjing Li,Yunan Gao,Jiacheng Yi,Qiye Li,Xiaoxiao Wang,Liya Wei,Le Kang

International journal of molecular sciences 26: PubMed40869273

2025

and the -COMPASS-like Complex Regulate Cardiac Progenitor Cell Division in the Embryonic Heart Tube.

Applications

Unspecified application

Species

Unspecified reactive species

Adam J Farmer,Mark H Inlow,Shaad M Ahmad,Kristopher R Schwab

eLife 13: PubMed40875449

2025

NAB2-STAT6 drives an EGR1-dependent neuroendocrine program in solitary fibrous tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Connor Hill,Alexandra Indeglia,Francis Picone,Maureen E Murphy,Cara Cipriano,Robert G Maki,Alessandro Gardini

Nature communications 16:6900 PubMed40715155

2025

SMCHD1 maintains heterochromatin, genome compartments and epigenome landscape in human myoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Huang,Wei Cui,Ishara Ratnayake,Kristin L Gallik,Lorna Cohen,Rabi Tawil,Gerd P Pfeifer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

ChIP - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

ChIP - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1277)

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