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Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877) is a rabbit monoclonal antibody that is used to detect Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ChIP, PepArr. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 20 publications


Images

ChIP - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (AB176877), expandable thumbnail
  • ChIC/CUT&RUN sequencing - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (AB176877), expandable thumbnail
  • ChIP-sequencing - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (AB176877), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (AB176877), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (AB176877), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBChIC/CUT&RUN-seqChIPPepArrICC/IFChIP-seqIHC-P
Human
Tested
Tested
Tested
Expected
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Expected
Expected
Tested
Rat
Expected
Expected
Expected
Expected
Expected
Expected
Tested
Synthetic peptide
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

-

Species
Human
Dilution info
1/5000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
2 µg for 105 Cells
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Use 2 μl.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
2 µg for 107 Cells
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Recommended products

Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877) is a rabbit monoclonal antibody that is used to detect Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ChIP, PepArr. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 20 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
ERP16597
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP, Peptide Array in Human, Mouse, Rat samples.

What is the molecular weight of Histone H3?


Anti-Histone H3 (mono methyl K4) [ERP16597] - ChIP Grade (ab176877) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa.

Trusted by the scientific community


Anti-Histone H3 (mono methyl K4) [ERP16597] - ChIP Grade (ab176877) was first used in a scientific publication in 2014 and has been cited over 20 times in peer-reviewed journals.

Reviewed by scientists


Anti-Histone H3 (mono methyl K4) [ERP16597] - ChIP Grade (ab176877) has over 5 independent reviews from customers.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [ERP16597] also available for your convenience:
ab176877, Carrier free - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade - BSA and Azide free ab239402, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Histone H3 (mono methyl K4) antibody [ERP16597] ab279693, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Histone H3 (mono methyl K4) antibody [ERP16597] ab283349



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • ChIP - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877), expandable thumbnail

    ChIP - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    Chromatin was prepared from HeLa (human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min.

    The ChIP was performed with 25 μg of chromatin, 2 μg of ab176877 (red), or 2 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).

  • ChIC/CUT&RUN sequencing - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    CUT&RUN was performed using the ChIC/CUT&RUN pAG-MNAse ChIC/CUT&RUN pAG-MNase ab285373, 2.5 x 10^5 HeLa cells and 2μg of ab176877 [ERP16597]. The resulting DNA was sequenced on the NovaSeq 6000 (PE150).
    Additional screenshots of mapped reads can be downloaded here.

  • ChIP-sequencing - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877), expandable thumbnail

    ChIP-sequencing - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 4 μg of ab176877 [ERP16597]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
    Additional screenshots of mapped reads can be downloaded here.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (mono methyl K4) with ab176877 at 1/1000 dilution, followed by goat anti-rabbit Alexa Fluor® 488 (IgG) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab176877 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (goat anti-mouse Alexa Fluor® 594 secondary) at 1/500 dilution.
    2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (goat anti-rabbit Alexa Fluor® 488; IgG H&L) at 1/400 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Histone H3 (mono methyl K4) with ab176877 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of colon tissue is observed. Counterstained with hematoxylin.

    Negative control: PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877), expandable thumbnail

    Western blot - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877) at 1/5000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates at 10 µg

    Lane 2: NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysates at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

  • Peptide Array - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877), expandable thumbnail

    Peptide Array - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    ab176877 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Histone H3 (mono methyl K4) with ab176877 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of colon tissue is observed. Counterstained with hematoxylin.

    Negative control: PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Histone H3 (mono methyl K4) with ab176877 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of colon tissue is observed. Counterstained with hematoxylin.

    Negative control: PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • ChIC/CUT&RUN sequencing - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 2 µg of ab176877 [ERP16597]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    The ChIP data was conducted on chromatin prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 4 µg of ab176877. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

    Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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