AB205256

Anti-Histone H3 (mono methyl K4) antibody [RM140]

Name: Anti-Histone H3 (mono methyl K4) antibody [RM140] (ab205256) | Abcam
Brand: Abcam Limited
SKU: AB205256
Rating: 5 (1 reviews)

Recombinant
What is this?

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal H3 mono methyl K4 antibody. Suitable for ChIP, ELISA, WB, ICC/IF and reacts with Human, Synthetic peptide samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H3C1 mono methyl K4.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me, H3K4me1, H3K4

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells treated with sodium butyrate, labelling Histone H3 (mono methyl K4) with ab205256 at 1 – 2 μg/mL (red). Actin filaments have been labeled with fluorescein phalloidin (green).

ChIP - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

ChIP

Supplier Data

ChIP - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

ChIP performed on HeLa cells using ab205256 (5ug). Real-time PCR was performed using primers specific to the gene indicated.

ELISA - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

ELISA

Supplier Data

ELISA - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

ab205256 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.

Supplier Data

Western blot - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

All lanes:

Western blot - Anti-Histone H3 (mono methyl K4) antibody [RM140] (ab205256) at 0.5 µg/mL

Lane 1:

Recombinant histone H3.3

Lane 2:

Acid extracts of HeLa cells

Predicted band size: 15 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM140

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ELISA, ICC/IF, ChIP, WB

applications

Immunogen

Synthetic Peptide within Human H3C1 mono methyl K4. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

ab205256 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-10 µg/mL", "ChIP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.2-1 µg/mL", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab205256 was purified from an animal origin–free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 mono methyl K4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 21:45 PubMed39737125

2024

Knockdown of ribosomal protein L22-like 1 arrests the cell cycle and promotes apoptosis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chunming Li,Xinna Du,Hu Zhang,Shuang Liu

Investigative ophthalmology & visual science 60:4619-4631 PubMed31675426

2019

Characterization of LSD1 Expression Within the Murine Eye.

Applications

Unspecified application

Species

Unspecified reactive species

Salma Ferdous,Hans E Grossniklaus,Jeffrey H Boatright,John M Nickerson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (mono methyl K4) antibody [RM140]

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

ChIP - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

ELISA - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

Western blot - Anti-Histone H3 (mono methyl K4) antibody [RM140] (AB205256)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise